Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (2): 52-60.doi: 10.6040/j.issn.1671-7554.0.2018.1388

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Effects of human placenta mesenchymal stem cell transplantation on ovarian function in premature ovarian failure in rats

HOU Qiaoni1,2, MA Huiming1,2, XIANG Li1,2, HE Yantao1,2, XU Xian2,3, CHEN Dongmei1,5, ZHANG Xueyu4   

  1. 1. Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Yinchuan 750004, Ningxia, China;
    3. Center for Reproductive Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    4. Department of Gynecology, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    5. Institute of Human Stem Cells, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Published:2022-09-27

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Abstract: Objective To investigate the antioxidant potential of human placenta mesenchymal stem cell(hPMSC)transplantation on the silent information regulator transcript-1(SIRT1)and nuclear factor-erythroid 2-related factor 2(Nrf2)and peroxisome proliferator-activated receptor gamma coactivator1-α(PGC1-α)in the treatment of premature ovarian failure(POF)in rats. Methods A total of 60 SD female rats with normal estrous cycle were randomly divided into 4 groups(n=15): blank control group, POF model group, hPMSC transplantation group, and treatment control group. The POF models were established by intraperitoneal injection of cyclophosphamide. The serum levels of estradiol(E2), follicle-stimulating hormone(FSH)and anti-müllerian hormone(AMH)were determined with ELISA. The fibrosis and histomorphological features of ovarian tissues were observed with Van Gieson(VG)staining and HE staining. The expressions of SIRT1, Nrf2 and PGC1-α were detected with Western blotting and immunohistochemical staining. Results Serum FSH level was significantly higher in the POF model group than in blank control group(P<0.05), while E2 and AMH levels were significantly lower(P<0.01). Compared with the treatment control group and POF model group, the hPMSC transplantation group had decreased FSH level and increased AMH level(P<0.01). HE staining showed that in the POF model group, the numbers of primordial follicles and primary follicles increased, while the number of atretic follicles decreased; in the hPMSC transplantation group, the number of normal follicles increased, while the number of atretic follicles decreased. Immunohistochemical staining results showed that the protein expressions of SIRT1, Nrf2 and PGC1-α were significantly lower in the POF model group than in the blank control group, and significantly higher in the hPMSC transplantation group than in the POF model group. Western blotting results showed the same tendency of changes(P<0.01). Conclusion The hPMSC transplantation may have antioxidant potential and protective effects on ovarian by activating the SIRT1/PGC1-α/Nrf2 signaling pathway.

Key words: Human placenta mesenchymal stem cell, silent information regulator transcript-1, peroxisome proliferator-activated receptor gamma coactivator1-α, nuclear factor-erythroid 2-related factor 2, Chemotherapy, Premature ovarian failure, Rat

CLC Number: 

  • R711.6
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