JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (9): 48-53.doi: 10.6040/j.issn.1671-7554.0.2014.033

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Effect of Ghrelin on the VEGF expression in steroid-induced avascular necrosis of the femoral head in rabbits

HUANG Laigang, CUI Baojuan, WANG Xiaohong, WANG Daoqing, ZENG Fanshuo, SUN Min, LIU Benling, SUN Qiangsan   

  1. Department of Rehabilitation, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2014-01-14 Revised:2014-09-04 Online:2014-09-10 Published:2014-09-10
  • Contact: 孙强三。E-mail:sunqsan@126.com E-mail:sunqsan@126.com

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Abstract: Objective To observe the effect of Ghrelin on the expression of vascular endothelial growth factor (VEGF) in steroid-induced avasular necrosis of the femoral head in rabbits. Methods A total of 34 male New-Zealand rabbits which aged more than 24 weeks were divided into normal group (n=8) and model group (n=26). Models of steroid-induced avascular necrosis of femoral head were induced by horse serum combined with hormone methylprednisolone. After that, 4 rabbits were selected from each group randomly to determine with MRI and histopathological examinations if the modeling was successful. The 20 successfully modeled rabbits were divided into control group (n=10) and Ghrelin intervention group (n=10), and the remaining 4 in the normal group were classified as blank group. Rabbits in Ghrelin intervention group received Ghrelin injection (25 μg/kg·d) for 2 weeks, and rabbits in control group were given the same dose of saline for 2 weeks. Samples were collected after the animals were sacrificed. The histomorphology of femoral head was examined, and VEGF mRNA level was detected with Real-time PCR. Results The control group displayed extensive sparse and broken trabecula, and adipocytes were fused. The Ghrelin intervention group showed occasionally broken sparse trabecula. The adipocytes were slightly hypertrophic but not fused, with a higher proportion in the marrow than in the control group. VEGF mRNA expression was higher in Ghrelin intervention group and controlgroup than in blank group (P<0.05), and it was higher in the Ghrelin intervention group than in the control group (P<0.05). Conclusion Ghrelin can up-regulate the expression of VEGF in steroid-induced avasular necrosis of femoral head in rabbits, and improve the microcirculation and progression of femoral head.

Key words: Avascular necrosis of the femoral head, Ghrelin, Rabbits, Vascular Endothelial Growth Factor, Magnetic Resonance Imaging

CLC Number: 

  • R493
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