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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (6): 71-75.doi: 10.6040/j.issn.1671-7554.0.2020.1648

• 临床医学 • 上一篇    下一篇

不稳定心绞痛患者亚甲基四氢叶酸还原酶基因多态性与血清同型半胱氨酸的相关性

张琳1,王燕2,马旭2,范雪松2,袁慧2   

  1. 1.北京林业大学医院检验科, 北京 100083;2.首都医科大学附属北京安贞医院检验科, 北京 100029
  • 发布日期:2021-06-10
  • 通讯作者: 袁慧. E-mail:18911612931@189.cn
  • 基金资助:
    国家自然科学基金面上项目(81770353)

Association between MTHFR polymorphism and serum homocysteine in patients with unstable angina pectoris

ZHANG Lin1, WANG Yan2, MA Xu2, FAN Xuesong2, YUAN Hui2   

  1. 1. Department of Clinical Laboratory, Beijing Forestry University Hospital, Beijing 100083, China;
    2. Department of Clinical Laboratory, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
  • Published:2021-06-10

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摘要： 目的 探讨冠心病不稳定心绞痛(UAP)患者亚甲基四氢叶酸还原酶(MTHFR)基因多态性与血清同型半胱氨酸(Hcy)水平之间的关系。 方法 选取2019年1月至2019年6月北京安贞医院收治的不稳定心绞痛患者243例(心绞痛组),同期健康查体志愿者269例(健康对照组),采用双试剂循环酶法检测两组血清Hcy水平,通过聚合酶链式反应和荧光法检测MTHFR基因多态性,采用t检验比较两组血清Hcy水平与 MTHFR基因多态性差异;MTHFR基因多态性根据PCR扩增产物分为CT型、CC型、TT型。 结果 心绞痛组血清Hcy总体水平显著高于健康对照组 ［(13.37±7.72)μmol/L vs(11.50±5.05)μmol/L,P<0.05］;心绞痛组CT基因型的Hcy水平显著高于健康对照组 ［(13.08±7.33)μmol/L vs(10.78±2.45)μmol/L,P<0.05 ］;心绞痛组和健康对照组MTHFR C677T基因型分布频率差异无统计学意义(P>0.05),心绞痛组患者的T等位基因分布频率明显高于健康对照组(59.1% vs 52.5%, P<0.05)。 结论 不稳定心绞痛患者Hcy水平显著高于健康对照组;其MTHFR C677T基因多态性与不稳定心绞痛疾病的发生无明显相关性,但等位基因T与该病的发生可能有一定关系。

关键词: 不稳定心绞痛, 亚甲基四氢叶酸还原酶, 同型半胱氨酸, 基因多态性, 心绞痛

Abstract: Objective To investigate the relationship between methylenetetrahydrofolate reductase(MTHFR)gene polymorphism and serum homocysteine(Hcy)level in patients with unstable angina pectoris(UAP). Methods A total of 243 UAP patients(UAP group)and 269 healthy controls were selected during Jan. 2019 and Jun. 2019 in Beijing Anzhen Hospital. The serum Hcy level of the two groups was detected with double reagent circulating enzyme method, and the MTHFR gene polymorphism was detected with polymerase chain reaction and fluorescence method. The differences in serum Hcy level and MTHFR gene polymorphism between the two groups were compared with t test. MTHFR gene polymorphisms were classified into CT, CC and TT according to PCR products. Results The overall level of serum Hcy in UAP group was significantly higher than that in healthy control group ［(13.37±7.72)μmol/L vs(11.50±5.05)μmol/L, P<0.05］. The Hcy level of CT genotype in UAP group was significantly higher than that in healthy control group ［(13.08±7.33)μmol/L vs(10.78±2.45)μmol/L, P<0.05］. There was no difference in the distribution frequency of MTHFR C677T genotype(P>0.05), but the distribution frequency of T allele in the UAP group was higher than that in healthy control group(59.1% vs 52.5%, P<0.05). Conclusion The level of Hcy in UAP patients is significantly higher than that in healthy controls. There is no significant correlation between MTHFR C677T gene polymorphism and UAP, but T allele may be related to UAP.

Key words: Unstable angina pectoris, Methylenetetrahydrofolate reductase, Homocysteine, Gene polymorphism, Angina pectoris

中图分类号: 

  • R446
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