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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (6): 64-70.doi: 10.6040/j.issn.1671-7554.0.2021.0076

• 临床医学 • 上一篇    下一篇

miR-33a在稳定型心绞痛患者血清中的表达及意义

张明明1,高伟1,崔佳1,霍丽静1,胡晴川1,李伟皓2,王超3   

  1. 1. 河北省人民医院检验科, 河北 石家庄 050051;2. 河北医科大学第二医院特检科, 河北 石家庄 050000; 3. 河北省人民医院临床医学研究中心, 河北 石家庄 050051
  • 发布日期:2021-06-10
  • 通讯作者: 张明明. E-mail:zhangmm197612@126.com
  • 基金资助:
    河北省科技厅重点研发计划项目民生科技专项(20377776D)

Expression and significance of serum miR-33a in patients with stable angina pectoris

ZHANG Mingming1, GAO Wei1, CUI Jia1, HUO Lijing1, HU Qingchuan1, LI Weihao2, WANG Chao3   

  1. 1. Department of Clinical Laboratory, Hebei General Hospital, Shijiazhuang 050051, Hebei, China;
    2. Department of Special Laboratory, the Second Hospital of Hebei Medical University, Shijiazhuang 050050, Hebei, China;
    3. Clinical Mdicine Research Center, Hebei General Hospital, Shijiazhuang 050051, Hebei, China
  • Published:2021-06-10

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摘要： 目的 探讨微小RNA-33a(miR-33a)在稳定型心绞痛患者血清中水平及检测价值。 方法 选取2018年1月至2019年12月确诊的稳定型心绞痛患者220例(心绞痛组),选取同期健康体检者200例(对照组)。心绞痛组按疾病严重程度分为Ⅰ、Ⅱ、Ⅲ、Ⅳ级。应用实时定量PCR法检测两组血清miR-33a表达水平;同时检测两组血清的血脂和炎性因子水平。采用GEO数据库检索数据,对miR-33a的功能进行生物信息学分析。 结果 心绞痛组血清miR-33a水平明显高于对照组(P<0.05)。不同分级的心绞痛患者血清miR-33a水平差异有统计学意义(P<0.05)。心绞痛组血脂指标中血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白、极低密度脂蛋白水平高于对照组,而高密度脂蛋白水平则低于对照组(P<0.05)。心绞痛组血清炎性因子指标中肿瘤坏死因子(TNF-α)、高敏C-反应蛋白(hsCRP)、白细胞介素-6(IL-6)的水平高于对照组。心绞痛组血清miR-33a与低密度脂蛋白、极低密度脂蛋白均呈正相关,与高密度脂蛋白呈负相关(P<0.05)。心绞痛组血清miR-33a水平与TNF-α、IL-6呈正相关(P<0.05)。生物信息学分析显示,miR-33a具有调控一些转录组基因的功能,参与核酸代谢、信号传导、细胞生长、氨基酸转运等过程。 结论 miR-33a在稳定型心绞痛患者血清中存在表达增高现象,与一些血脂及炎性因子指标有相关性,miR-33a可能通过较为复杂的分子网络机制调控各项指标在稳定型心绞痛的病情进展中发挥作用。

关键词: 稳定型心绞痛, 微小RNA-33a, 血脂, 炎性因子

Abstract: Objective To investigate the level and significance of serum miR-33a in patients with stable angina pectoris(SAP). Methods A total of 220 SAP patients treated during Jan. 2018 and Dec. 2019 were recruited as the SAP group, and 200 healthy cases were enrolled as control group. The SAP patients were divided into Ⅰ, Ⅱ, Ⅲ, Ⅳ according to the severity of the disease. The level of miR-33a in both groups was detected with real time PCR, and serum lipids and inflammatory factors were detected. Data in the GEO database was retrieved for bioinformatics analysis on the function of miR-33a. Results The level of serum miR-33a in the SAP group was significantly higher than that in control group(P<0.05). There were significant differences in miR-33a among patients with different severity of SAP(P<0.05). The levels of serum total cholesterol(TC), triglycerides(TG), low-density lipoprotein(LDLC), and very low-density lipoprotein(VLDL)were higher in the SAP group than in control group, while high-density lipoprotein(HDLC)level was lower(P<0.05). The serum inflammatory factors, including tumor necrosis factor(TNF-α), high-sensitivity C-reactive protein(hsCRP), and interleukin-6(IL-6), in SAP group were higher than those in control group(P<0.05). Level of serum miR-33a in SAP patients was positively correlated with LDL and VLDL, but was negatively correlated with HDL(P<0.05). Level of serum miR-33a in SAP patients was positively correlated with TNF-α and IL-6(P<0.05). Bioinformatic analysis showed that miR-33a was involved in regulating transcriptome genes, nucleic acid metabolism, signal transduction, cell growth, amino acid transport and other processes. Conclusion The expression of miR-33a increases in SAP patients, which is related to some lipids and inflammatory factors. miR-33a may play a role in the progression of SAP by regulating these indicators via complex molecular network mechanism.

Key words: Stable angina pectoris, miR-33a, Serum lipids, Inflammatory factors

中图分类号: 

  • R541.4
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