红景天苷对人胃癌细胞SGC-7901抑瘤作用的体外实验研究

doi:10.6040/j.issn.1671-7554.0.2015.131

摘要/Abstract

摘要： 目的研究红景天苷对体外培养的人胃癌细胞SGC-7901增殖及生长的影响,并探讨其抗肿瘤作用的可能机制。方法实验分为对照组、0.50 mg/mL红景天苷组、1.00 mg/mL红景天苷组及2.00 mg/mL红景天苷组。通过MTT比色法、核仁组成区嗜银蛋白(AgNOR)染色、流式细胞技术、细胞划痕实验的方法检测不同浓度红景天苷对SGC-7901细胞增殖、AgNOR表达、细胞周期、细胞凋亡及细胞迁移作用的影响。结果与对照组相比,不同浓度的红景天苷组SGC-7901细胞增殖及AgNOR表达均受到明显抑制(P<0.05),结果呈明显的浓度-时间-效应依赖关系;细胞停滞在S期的比例减少、G₂/M期的比例增加(P<0.05);细胞凋亡率升高,迁移率降低(P<0.05)。结论红景天苷能抑制SGC-7901细胞增殖,并能阻滞细胞周期和诱导细胞凋亡,降低胃癌细胞的迁移能力。

关键词: 红景天苷, 胃癌细胞, 增殖, 抗肿瘤

Abstract: Objective To investigate the effect of salidroside on the proliferation and growth of in vitro cultured human gastric carcinoma SGC-7901 cells, and to explore the possible anti-tumor mechanism. Methods Cells were divided into control group, 0.50 mg/mL salidroside group, 1.00 mg/mL salidroside group and 2.00 mg/mL salidroside group. Cell proliferation was assessed by MTT array. Argyrophilic nucleolar organiser region proteins (AgNOR) staining was used to determine the expression of AgNOR in nuclear. The cell cycle and apoptosis were detected by flow cytometry test. Migration rate was measured by cell scratch assay. Results Compared with control group, the proliferation and AgNOR expression of SGC-7901 cells were inhibited significantly in salidroside groups (P<0.05) with a concentration-, time-and effect-depent manner; cell circle arrest increased at G₂/M phase, but decreased at S phase (P<0.05); the rate of cell apoptosis increased, while cell migration rate was down-regulated (P<0.05). Conclusion Salidroside can effectively inhibit proliferation, arrest cell cycle, induce apoptosis and decrease migration rate of SGC-7901 cell.

Key words: Gastric carcinoma cells, Proliferation, Salidroside, Anti-tumor

中图分类号:

R331

韦丽兰, 莫书荣. 红景天苷对人胃癌细胞SGC-7901抑瘤作用的体外实验研究[J]. 山东大学学报（医学版）, 2015, 53(12): 12-15,32.

WEI Lilan, MO Shurong. An in vitro study of the anticancer effect of salidroside on human gastric carcinoma SGC-7901 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 12-15,32.

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