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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (12): 7-11.doi: 10.6040/j.issn.1671-7554.0.2015.025

• 基础医学 • 上一篇    下一篇

酪酪肽对胰腺癌Miapaca-2细胞凋亡的影响

李红霞1, 董蕾2, 姜炅2, 王新阳3   

  1. 1. 西安交通大学第一附属医院消化内科, 陕西西安 710061;
    2. 西安交通大学第二附属医院消化内科, 陕西西安 710004;
    3. 西安交通大学第一附属医院泌尿外科实验室, 陕西西安 710061
  • 收稿日期:2015-01-08 出版日期:2015-12-10 发布日期:2015-12-10
  • 通讯作者: 李红霞。E-mail:hongxia1105@126.com E-mail:hongxia1105@126.com
  • 基金资助:
    国家自然科学基金(81170352)

Effects of peptide YY on apoptosis of human pancreatic cancer cell line Miapaca-2 in vitro

LI Hongxia1, DONG Lei2, JIANG Jiong2, WANG Xinyang3   

  1. 1. Department of Gastroenterology, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China;
    2. Department of Gastroenterology, the Second Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China;
    3. Urological Research Laboratory, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China
  • Received:2015-01-08 Online:2015-12-10 Published:2015-12-10

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摘要： 目的 观察酪酪肽(PYY)对体外培养的胰腺癌Miapaca-2细胞凋亡的影响。方法 不同浓度(0.01、0.005、0.0025、0.00125 mmol/L) PYY分别作用于体外常规培养的Miapaca-2细胞48 h后,流式细胞仪检测细胞凋亡率、细胞周期;RT-PCR法检测Survivin mRNA,Western blotting法检测Survivin、Caspase-3蛋白的表达。结果 PYY处理48 h后细胞凋亡率明显升高,并呈浓度依赖性;G0/G1期细胞数明显增多,S、G2/M期细胞数明显减少,G1期前出现亚二倍体凋亡峰;PYY明显抑制Survivin蛋白和mRNA表达(P<0.01),上调Caspase-3蛋白表达(P<0.01)。结论 PYY能诱导胰腺癌Miapaca-2细胞凋亡,参与细胞周期调控,其机制可能与抑制Survivin蛋白和mRNA表达、上调Caspase-3蛋白表达有关。

关键词: Caspase, 酪酪肽, 胰腺癌细胞, 细胞凋亡, Survivin

Abstract: Objectives To observe the effects of peptide YY(PYY)on inducing apoptosis of human pancreatic cancer cell line Miapaca-2. Methods The pancreatic cancer cell line Miapaca-2 was treated with PYY of different concentrations(0.01, 0.005, 0.0025 and 0.00125 mmol/L) for 48 h.The apoptosis and cell cycle were evaluated by flow cytometry, the expression of survivin mRNA was detected with RT-PCR, and the expressions of survivin and Caspase-3 protein were detected with Western blotting. Results After intervention with PYY for 48 h, celluar apoptosis rate was significantly increased in a dose-dependent manner. The cells of G0/G1 stage were significantly increased, but the cells of S and G2/M stages were significantly decreased. The hypodiploid apoptotic peak before the G1 stage was detected. PYY inhibited survivin protein and survivin mRNA expressions but increased Caspase-3 protein expression notably in a dose dependent manner(P<0.01). Conclusion PYY can induce apoptosis in a dose-dependent manner. PYY maybe block cell cyclein G0/G1 stage, downregulate survivin mRNA and protein expressions, increase Caspase-3 protein expression and finally induce apoptosis.

Key words: Peptide YY, Apoptosis, Pancreatic cancer cell, Survivin, Caspase

中图分类号: 

  • R735.9
[1] 潘淑波,刘欣.胃肠激素酪酪肽的特性及其与疾病关系的研究进展[J].陕西医学杂志,2009,10:1382-1383. PAN Shubo, LIU Xin. Research progress of the characteristics of peptide YY and its relation with disease[J]. Shaanxi Medical Journal, 2009, 10:1382-1383.
[2] Alosi JA, McFadden DW. Peptide YY mediates inhibition of tumor growth and inflammation[J]. Methods Mol Biol, 2009, 512:377-394.
[3] Matsunami K, Takagi H, Ichigo S, et al. Peptide YY producing strumal carcinoid tumor of the ovary[I]. Eur J Gynaecol Oncol, 2011, 32(2):201-202.
[4] Vona-Davis L, McFadden DW. PYY and pancreas:inhibition of tumor growth[J]. Peptides, 2007, 28(2):334-338.
[5] 李媛,董蕾.酪酪肽与消化道肿瘤[J].国际消化病杂志,2007,27(1):52-54. LI Yuan, DONG Lei. Peptide YY and alimentary canal cancer[J]. International Journal of Digestive Disease, 2007, 27(1):52-54.
[6] 李媛,董蕾.酪酪肽对荷人肝癌裸鼠皮下移植瘤的影响[J].南方医科大学学报,2008,28(8):1442-1445. LI Yuan, DONG Lei. Effect of peptide YY on subcutaneous transplantation tumor of human hepatoma in nude mice[J]. Journal of Southern Medical University, 2008, 28(8):1442-1445.
[7] 李红霞,董蕾,姜炅,等.酪酪肽对胰腺癌细胞生长的抑制作用及Y受体在胰腺癌细胞的表达[J].西安交通大学学报:医学版,2011,32(6):713-716. LI Hongxia, DONG Lei, JIANG Jiong, et al. Inhibitory effect of peptide YY and expression of Y receptors on human pancreatic carcinoma Miapaca-2 cells[J]. Journal of Xi'an Jiaotong University:Medical Sciences, 2011, 32(6):713-716.
[8] 吴金华,杨细虎,刘振中,等.凋亡抑制因子Survivin在口腔鳞癌中的表达及其意义[J].山东大学学报:医学版,2014,52(4):89-92. WU Jinhua, YANG Xihu, LIU Zhenzhong, et al. Expression and significance of Survivin in human oral squamous cell carcinoma[J]. Journal of Shandong University:Health Sciences, 2014, 52(4):89-92.
[9] 徐冬,许利剑.凋亡抑制基因Survivin在胰腺癌中的研究进展[J].医学研究生学报,2013,26(3):319-322. XU Dong, XU Lijian. Progress of anti-apoptosis gene survivin in pancreatic cancer[J]. Journal of Medical Graduate Students, 2013, 26(3):319-322.
[10] Xie H, Jiang W, Xiao SY, et al. High expression of survivin is prognostic of shorter survival but not predictive of adjuvant gemcitabine benefit in patients with resected pancreatic adenocarcinoma[J]. J Histochem Cytochem, 2013, 61(2):148-155.
[11] 任泽强,张蓬波,丁伟超,等.USP9X、Survivin在胰腺癌组织中的表达及临床意义[J].世界华人消化杂志,2014,22(33):5164-5169. REN Zeqiang, ZHANG Pengbo, DING Weichao, et al. Clinical signifiance of expression of ubiquitin-specifi protease 9X and Survivin in pancreatic carcinoma[J]. World Chinese Journal of Digestology, 2014, 22(33):5164-5169.
[12] Yang J, Ouyang J, Ouyang L, et al. Inhibition of cell proliferation and increase of chemosensitivity by simultaneous knockdown of XIAP and survivin in pancreatic carcinoma cells[J]. Oncol Res, 2013, 21(1):43-50.
[13] 易小平,江春,宰红艳,等.慢病毒载体介导RNAi靶向抑制胰腺癌细胞survivin表达并诱导细胞凋亡的实验研究[J].中国普通外科杂志,2010,19(3):227-233. YI Xiaoping, JIANG Chun, ZAI Hongyan, et al. Lentiviral vector-mediad RNA interference targeted against survivin inhibits survivin expression and induces cell apoptosis of human pancreatic cancer in vitro[J]. Chinese Journal of General Surgery, 2010, 19(3):227-233.
[14] 曲贝贝,左金华.凋亡抑制基因Survivin的研究进展[J].中华临床医师杂志,2014,8(15):2842-2846. QU Beibei, ZUO Jinhua. Research progress of apoptosis inhibiting gene Survivin[J]. Chin J Clinicians, 2014, 8(15):2842-2846.
[15] 刘园园,王逸飞,张春敏,等. ALA-PDT对皮肤鳞状细胞癌A431细胞Caspase-3及Caspase-7表达的影响[J].山东大学学报:医学版,2014,52(12):50-53,59. LIU Yuanyuan, WANG Yifei, ZHANG Chunmin, et al. Effects of ALA-PDT on Caspase-3 and Caspase-7 expressions in cutaneous squamous cell carcinoma A431 cells[J]. Journal of Shandong University:Health Sciences, 2014, 52(12):50-53,59.
[16] Xu JH, Wang AX, Huang HZ, et al. Survivin in shRNA induces caspase-3-dependent apoptosis and enhances cisplation sensitivity in squamous cell carcinoma of the tongue[J]. Oncol Res, 2010, 18(8):377-385.
[17] 孙建建,李胜棉,赵松,等.Survivin和Casepase-3在胰腺癌组织中的表达及与预后的关系[J].肿瘤防治研究,2012,39(1):62-67. SUN Jianjian, LI Shengmian, ZHAO Song, et al. Expression and prognostic significance of Survivin and Caspase-3 in pancreatic carcinoma[J]. Cancer Research on Prevention and Treatment, 2012, 39(1):62-67.
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