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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (6): 48-53.doi: 10.6040/j.issn.1671-7554.0.2014.560

• 基础医学 • 上一篇    下一篇

沉默PADI4基因对卵巢癌细胞系OVCAR3的作用

周静1,2, 常晓天2, 周婷2, 崔莹莹2, 张蓓2, 荣风年2   

  1. 1. 山东大学医学院, 山东 济南 250012;
    2. 山东大学附属千佛山医院妇产科, 山东 济南 250014
  • 收稿日期:2014-10-10 修回日期:2015-03-06 出版日期:2015-06-10 发布日期:2015-06-10
  • 通讯作者: 荣风年。 E-mail:fnrong@163.com E-mail:fnrong@163.com
  • 基金资助:
    山东省自然科学基金(ZR2013HM037)

Effects of PADI4 silence on ovarian cancer cell line OVCAR3

ZHOU Jing1,2, CHANG Xiaotian2, ZHOU Ting2, CUI Yingying2, ZHANG Bei2, RONG Fengnian2   

  1. 1. School of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Gynecology and Obstetrics, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China
  • Received:2014-10-10 Revised:2015-03-06 Online:2015-06-10 Published:2015-06-10

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摘要： 目的 探讨抑制肽酰基精氨酸脱亚氨酶4(PADI4)对卵巢癌细胞系OVCAR3的影响。方法 PADI4-siRNA组和阴性对照组siRNA分别转染卵巢癌细胞OVCAR3,采用实时定量PCR及Western Blotting检测PADI4、p53和p21的表达, AnnexinⅤ-PE/7-AAD双染和transwell小室法分别检测细胞凋亡、侵袭和迁移的情况。结果 与阴性对照组相比,PADI4-siRNA组的OVCAR3细胞中PADI4表达降低,且其凋亡细胞比例明显增加(P<0.05),细胞侵袭能力明显减弱(P<0.01),但细胞趋化能力没有明显变化。PADI4-siRNA组p53表达较阴性对照组降低,但p21表达并没有降低。结论 沉默PADI4影响卵巢癌细胞OVCAR3凋亡和侵袭特性,提示PADI4参与调控卵巢癌细胞恶性生物学行为。

关键词: 肽酰基精氨酸脱亚氨酶4, 卵巢肿瘤, 细胞凋亡, 侵袭, 迁移

Abstract: Objective To investigate the effects of PADI4 silence on ovarian cancer cell line OVCAR3. Methods OVCAR3 cells were transfected with siRNA of PADI4-siRNA group and negative control group in vitro respectively. The expressions of PADI4, p53 and p21 were detected with real-time PCR and Western blotting. Cell apoptosis, invasion and migration were measured with AnnexinV-PE/7-AAD assay and Transwell assay. Results Compared with that of the negative control group, PADI4 expression of OVCAR3 cells in PADI4-siRNA group decreased significantly, the invasion ability and apoptotic ratio also reduced (Pa<0.05, Pi<0.01). However, the chemotaxis ability showed no change. Compared with that of the negative control group, expression of p53 decreased, while p21 expression remained unchanged. Conclusion PADI4 silence altered the apoptosis and invasion abilities of ovarian cancer cell OVCAR3, demonstrating that PADI4 plays an important role in the control of malignant biological behavior of ovarian cancer.

Key words: Invasion, Apoptosis, Migration, Ovarian tumor, Peptidylarginine deiminase 4

中图分类号: 

  • R711.75
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