MRP8通过NF-κB信号通路调控痛风细胞模型中IL-1β的表达

doi:10.6040/j.issn.1671-7554.0.2014.490

摘要/Abstract

摘要： 目的研究髓样相关蛋白8(MRP8)在痛风中的作用及其机制。方法单钠尿酸盐(MSU)结晶诱导巨噬细胞产生痛风模型,检测诱导后巨噬细胞中MRP8的水平。MRP8 siRNA转染巨噬细胞,Western blotting检测沉默效果及MRP8沉默对核转录因子κB(NF-κB)信号通路中的NF-κB和NF-κB抑制蛋白(I-κB)表达的影响,ELISA法检测MRP8沉默对炎性因子白细胞介素-1β(IL-1β)分泌的影响。结果 MSU可诱导巨噬细胞高表达MRP8(P<0.01);MRP8 siRNA转染能有效干扰巨噬细胞中MRP8的表达。MPR8沉默后巨噬细胞中IL-1β的分泌明显减少(P<0.05),同时NF-κB表达下调,I-κB表达上调。结论 MRP8参与MSU诱导痛风的炎性因子IL-1β的释放过程,且有可能通过NF-κB信号通路调控IL-1β的分泌来实现。

Abstract: Objective To explore the effect and underlying mechanism of myeloid-related protein 8 (MRP8) on gout. Methods Gout model in macrophages was induced by monosodium urate (MSU), and the expression of MRP8 in these model cells was detected by ELISA. Then, MRP8 siRNA was transferred into macrophage, and the effectiveness was verified by Western blotting. The influences of silenced MRP8 on the secretion of interleukin-1β (IL-1β), nuclear transcription factor-κB (NF-κB) and inhibitor of nuclear transcription factor-κB (I-κB) expressions in macrophages were detected by ELISA and Western blotting. Results The expression of MRP8 in macrophages increased after MSU induction (P<0.01) and MPR siRNA could silence MPR8 expression effectively. After the silence of MPR8, the IL-1β secretion decreased (P<0.05), NF-κB expression was down-regulated, while I-κB espression was up-regulated in macrophages. Conclusion MRP-8 participates in the process of IL-1β release in MSU-induced macrophages gout model, which may be regulated by NF-κB signal pathway.

Key words: Nuclear transcription factor-κB, Interleukin-1β, Inhibitor of nuclear transcription factor-κB, Myeloid-related protein 8, Macrophage

中图分类号:

R589

赵璐, 孙俊波, 魏桂梅. MRP8通过NF-κB信号通路调控痛风细胞模型中IL-1β的表达[J]. 山东大学学报（医学版）, 2015, 53(6): 44-47.

ZHAO Lu, SUN Junbo, WEI Guimei. MRP8 regulates expression of IL-1β in gout cell model by NF-κB signaling pathway[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(6): 44-47.

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