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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (7): 43-47.doi: 10.6040/j.issn.1671-7554.0.2014.964

• 临床医学 • 上一篇    下一篇

Th22细胞联合Th17细胞在宫颈癌外周血中的表达及意义

田馨莉1, 矫俊1, 张腾1, 马道新2, 崔保霞1   

  1. 1. 山东大学齐鲁医院妇产科,山东 济南 250012;
    2. 山东大学齐鲁医院血液病研究室,山东 济南 250012
  • 收稿日期:2014-12-17 修回日期:2015-03-11 出版日期:2015-07-10 发布日期:2015-07-10
  • 通讯作者: 崔保霞。E-mail:cuibaoxia@sdu.edu.cn E-mail:cuibaoxia@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81172486);山东大学基本科研业务费(2014QLKY23)

Expression and significance of Th22 and Th17 cells in the peripheral blood of cervical cancer

TIAN Xinli1, JIAO Jun1, ZHANG Teng1, MA Daoxin2, CUI Baoxia1   

  1. 1. Department of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Institute of Hemopathy, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-12-17 Revised:2015-03-11 Online:2015-07-10 Published:2015-07-10

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摘要： 目的 探讨宫颈癌外周血中Th22细胞和Th17细胞的表达及其在宫颈癌发生发展中的作用。方法 取34例宫颈癌患者外周血为宫颈癌组、21例宫颈上皮内瘤变(CIN)患者的外周血为CIN组、19例健康志愿者外周血为对照组, 采用流式细胞术检测外周血中Th22、Th17细胞的表达比例。酶联免疫吸附试验(ELISA) 方法检测血浆中相关细胞因子白细胞介素-22(IL-22)的水平。结果 与对照组比较, Th22、Th17细胞比例(占淋巴细胞百分比)CIN组(P<0.01, P<0.05) 及宫颈癌组(P<0.01, P<0.01)均明显升高。Th22细胞比例宫颈癌组又明显高于CIN组(P<0.01)。CIN组中Th22细胞与Th17细胞呈正相关(r=0.41, P<0.05 ), 宫颈癌组Th22细胞与Th17细胞呈正相关(r=0.49, P<0.01)。与对照组相比, IL-22在宫颈癌组外周血中明显升高(P<0.05)。结论 Th22、Th17细胞在宫颈癌患者外周血中比例增高, 在宫颈癌的发生发展中可能起着重要作用。

关键词: 宫颈肿瘤, Th22细胞, 宫颈上皮内瘤变, Th17细胞, 白细胞介素-22

Abstract: Objective To determine the expressions of Th22 and Th17 cells in the peripheral blood of cervical cancer and their contribution in the occurrence and progression of cervical cancer. Methods Flow cytometry was used to determine the percentages of Th22 cells and Th17 cells in the peripheral blood of 34 cervical cancer (CC) patients, 21 cervical intraepithelial neoplasia (CIN) patients and 19 healthy controls. ELISA was adopted to examine the plasma level of interleukin-22(IL-22). Results The percentages of Th22 cells (all P<0.01) and Th17 cells (CIN:P<0.05; CC:P<0.01) in CIN and CC patients were significantly increased compared with those in controls. The percentage of Th22 cells in cervical cancer was higher than that in CIN patients (P<0.01). An positive correlation between Th22 cells and Th17 cells was found in CIN patients (r=0.41, P<0.05). Th22 cells showed a positive correlation with Th17 cells in CC patients (r=0.49, P<0.01). The level of IL-22 in plasma was significantly increased in CC patients compared with that in controls (P<0.05). Conclusion There is a high percentage of Th22 and Th17 cells in CC patients. Th22 and Th17 cells may jointly participate in the pathogenesis and progression of CC.

Key words: Cervical neoplasms, Cervical intraepithelial neoplasia, Th22 cells, Th17 cells, Interleukin-22

中图分类号: 

  • R737.33
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