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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (10): 55-59.doi: 10.6040/j.issn.1671-7554.0.2015.1101

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脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义

满琦1,张安1,张源2,樊明德1,马道新3,王成伟1   

  1. 1. 山东大学第二医院神经外科, 山东 济南 250033;2.山东大学齐鲁医院急诊科, 山东 济南 250012;3.山东大学齐鲁医院血液科, 山东 济南 250012
  • 收稿日期:2015-11-12 出版日期:2016-10-10 发布日期:2016-10-10
  • 通讯作者: 王成伟. E-mail:wangchengwei@sdu.edu.cn E-mail:wangchengwei@sdu.edu.cn
  • 基金资助:
    山东省自然科学基金(ZR2013HM083)

Expressions and significance of Th17 and Th22 cells in peripheral blood of patients with glioma

MAN Qi1, ZHANG An1, ZHANG Yuan2, FAN Mingde1, MA Daoxin3, WANG Chengwei1   

  1. 1. Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-12 Online:2016-10-10 Published:2016-10-10

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摘要： 目的 探究脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义。 方法 取34例脑胶质瘤患者及24例健康对照者为研究对象,用流式细胞术检测外周血中Th17、Th22细胞的表达比例。用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测外周血单个核细胞中视黄酸相关孤儿核受体(RORC)mRNA及芳香烃受体(AHR)mRNA的表达。用酶联免疫吸附分析(ELISA)检测血浆中Th22细胞相关因子白细胞介素-22(IL-22)的水平。 结果 与健康对照组相比,脑胶质瘤患者外周血Th17细胞比例(占淋巴细胞)升高不明显(P>0.05),且高级别组(WHO Ⅲ-Ⅳ级)与低级别组(WHO Ⅰ-Ⅱ级)之间差异不明显(P>0.05);Th22细胞比例(占淋巴细胞)明显升高(P<0.05),且不同级别之间差异明显(P<0.05)。脑胶质瘤患者Th17与Th22细胞呈正相关(r=0.40, P=0.03),健康对照组中二者无相关(P>0.05)。与健康对照组比较,患者外周血中RORC mRNA及AHR mRNA的升高不明显(P>0.05, P>0.05),且不同级别间差异不明显(P>0.05, P>0.05)。患者血浆IL-22水平比健康对照组明显升高(P<0.05),但不同级别之间差异不明显(P>0.05)。 结论 脑胶质瘤患者外周血中存在Th22细胞及IL-22异常升高,Th17细胞与Th22细胞可能共同参与胶质瘤的发生发展过程。

关键词: Th17细胞, 脑胶质细胞瘤, Th22细胞, 白细胞介素-22

Abstract: Objective To investigate the expression and significance of Th17 and Th22 cells in the peripheral blood of patients with glioma. Methods A total of 34 patients with glioma and 24 normal controls were enrolled. The percentages of Th17 and Th22 cells in peripheral blood were measured by flow cytometry. Retinoid-related orphan nuclear receptor(RORC)and arylhydrocarbon receptor(AHR)mRNA in peripheral blood mononuclear cells were detected by reverse-transcriptional polymerase chain reaction(RT-PCR). The plasma level of interleukin-22(IL-22)was detected by ELISA. Results There was no difference between patients and healthy controls in circulating Th17 cells(P>0.05), and no difference was found between the high-grade group(WHO Ⅲ-Ⅳ)and low-grade group(WHO Ⅰ-Ⅱ)(P>0.05). The percentage of circulating Th22 cells in glioma was significantly increased compared with that in controls(P<0.05), and the difference between different grade groups was obvious(P<0.05). A positive correlation between Th22 and Th17 cells was observed in glioma(r=0.40, P=0.03), no correlation was found in controls(P>0.05). The difference of RORC and AHR between patients and controls was not significant(P>0.05, P>0.05), 山 东 大 学 学 报 (医 学 版)54卷10期 -满琦,等.脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义 \=-and no difference was found between different grade groups(P>0.05, P>0.05). The serum IL-22 level in patients was significantly increased compared with that in controls(P<0.05). However, there was no difference of serum IL-22 levels between different grade groups(P>0.05). Conclusion There exist abnormal increases of Th22 cells and IL-22 levels in the peripheral blood of glioma, and Th17 and Th22 cells may jointly participate in the pathogenesis and progression of glioma.

Key words: Th17 cells, Th22 cells, Interleukin-22, Glioma

中图分类号: 

  • R739.41
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