您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (10): 55-59.doi: 10.6040/j.issn.1671-7554.0.2015.1101

• • 上一篇    下一篇

脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义

满琦1,张安1,张源2,樊明德1,马道新3,王成伟1   

  1. 1. 山东大学第二医院神经外科, 山东 济南 250033;2.山东大学齐鲁医院急诊科, 山东 济南 250012;3.山东大学齐鲁医院血液科, 山东 济南 250012
  • 收稿日期:2015-11-12 出版日期:2016-10-10 发布日期:2016-10-10
  • 通讯作者: 王成伟. E-mail:wangchengwei@sdu.edu.cn E-mail:wangchengwei@sdu.edu.cn
  • 基金资助:
    山东省自然科学基金(ZR2013HM083)

Expressions and significance of Th17 and Th22 cells in peripheral blood of patients with glioma

MAN Qi1, ZHANG An1, ZHANG Yuan2, FAN Mingde1, MA Daoxin3, WANG Chengwei1   

  1. 1. Department of Neurosurgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Hematology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-11-12 Online:2016-10-10 Published:2016-10-10

PDF (PC)

349

摘要： 目的 探究脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义。 方法 取34例脑胶质瘤患者及24例健康对照者为研究对象,用流式细胞术检测外周血中Th17、Th22细胞的表达比例。用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测外周血单个核细胞中视黄酸相关孤儿核受体(RORC)mRNA及芳香烃受体(AHR)mRNA的表达。用酶联免疫吸附分析(ELISA)检测血浆中Th22细胞相关因子白细胞介素-22(IL-22)的水平。 结果 与健康对照组相比,脑胶质瘤患者外周血Th17细胞比例(占淋巴细胞)升高不明显(P>0.05),且高级别组(WHO Ⅲ-Ⅳ级)与低级别组(WHO Ⅰ-Ⅱ级)之间差异不明显(P>0.05);Th22细胞比例(占淋巴细胞)明显升高(P<0.05),且不同级别之间差异明显(P<0.05)。脑胶质瘤患者Th17与Th22细胞呈正相关(r=0.40, P=0.03),健康对照组中二者无相关(P>0.05)。与健康对照组比较,患者外周血中RORC mRNA及AHR mRNA的升高不明显(P>0.05, P>0.05),且不同级别间差异不明显(P>0.05, P>0.05)。患者血浆IL-22水平比健康对照组明显升高(P<0.05),但不同级别之间差异不明显(P>0.05)。 结论 脑胶质瘤患者外周血中存在Th22细胞及IL-22异常升高,Th17细胞与Th22细胞可能共同参与胶质瘤的发生发展过程。

关键词: Th17细胞, 脑胶质细胞瘤, Th22细胞, 白细胞介素-22

Abstract: Objective To investigate the expression and significance of Th17 and Th22 cells in the peripheral blood of patients with glioma. Methods A total of 34 patients with glioma and 24 normal controls were enrolled. The percentages of Th17 and Th22 cells in peripheral blood were measured by flow cytometry. Retinoid-related orphan nuclear receptor(RORC)and arylhydrocarbon receptor(AHR)mRNA in peripheral blood mononuclear cells were detected by reverse-transcriptional polymerase chain reaction(RT-PCR). The plasma level of interleukin-22(IL-22)was detected by ELISA. Results There was no difference between patients and healthy controls in circulating Th17 cells(P>0.05), and no difference was found between the high-grade group(WHO Ⅲ-Ⅳ)and low-grade group(WHO Ⅰ-Ⅱ)(P>0.05). The percentage of circulating Th22 cells in glioma was significantly increased compared with that in controls(P<0.05), and the difference between different grade groups was obvious(P<0.05). A positive correlation between Th22 and Th17 cells was observed in glioma(r=0.40, P=0.03), no correlation was found in controls(P>0.05). The difference of RORC and AHR between patients and controls was not significant(P>0.05, P>0.05), 山 东 大 学 学 报 (医 学 版)54卷10期 -满琦,等.脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义 \=-and no difference was found between different grade groups(P>0.05, P>0.05). The serum IL-22 level in patients was significantly increased compared with that in controls(P<0.05). However, there was no difference of serum IL-22 levels between different grade groups(P>0.05). Conclusion There exist abnormal increases of Th22 cells and IL-22 levels in the peripheral blood of glioma, and Th17 and Th22 cells may jointly participate in the pathogenesis and progression of glioma.

Key words: Th17 cells, Th22 cells, Interleukin-22, Glioma

中图分类号: 

  • R739.41
[1] Ostrom QT, Gittleman H, Liao P, et al. CBTRUS statistical report: primary brain and central nervous system tumors diagnosed in the United States in 2007-2011[J]. Neuro Oncol, 2014, 16(Suppl 4): iv1-iv63.
[2] Rolle CE, Sengupta S, Lesniak MS. Challenges in clinical design of immunotherapy trials for malignant glioma[J]. Neurosurg Clin N Am, 2010, 21(1): 201-214.
[3] Galea I, Bernardes-Silva M, Forse PA, et al. An antigen-specific pathway for CD8 T cells across the blood-brain barrier[J]. J Exp Med, 2007, 204(9): 2023-2030.
[4] Harrington LE, Hatton RD, Mangan PR, et al. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type1 and 2 lineages[J]. Nat Immunol, 2005, 6(11): 1123-1132.
[5] Ivanov II, McKenzie BS, Zhou L, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells[J]. Cell, 2006, 126(6): 1121-1133.
[6] Trifari S, Kaplan CD, Tran EH, et al. Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells[J]. Nat Immunol, 2009, 10(8): 864-871.
[7] Duhen T, Geiger R, Jarrossay D, et al. Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells[J]. Nat Immunol, 2009, 10(8): 857-863.
[8] Muranski P, Boni A, Antony PA, et al. Tumor-specific Th17-polarized cells eradicate large established melanoma[J]. Blood, 2008, 112(2): 362-373.
[9] Wang L, Yi T, Kortylewski M, et al. IL-17 can promote tumor growth through an IL-6-Stat3 signaling pathway[J]. J Exp Med, 2009, 206(7): 1457-1464.
[10] Wainwright DA, Sengupta S, Han Y, et al. The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma[J]. PLoS One, 2010, 5(10): e15390. doi: 10.1371/journal.pone.0015390.
[11] Hu J, Mao Y, Li M, et al. The profile of Th17 subset in glioma[J]. Int Immunopharmacol, 2011, 11(9): 1173-1179.
[12] Wolk K, Witte E, Witte K, et al. Biology of interleukin-22[J]. Semin Immunopathol, 2010, 32(1):17-31.
[13] Zenewicz LA, Yancopoulos GD, Valenzuela DM, et al. Interleukin-22 but not interleukin-17 provides protection to hepatocytes during acute liver inflammation[J]. Immunity, 2007, 27(4): 647-659.
[14] Dumoutier L, Louahed J, Renauld JC. Cloning and characterization of IL-10-related T cell-derived inducible factor(IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9[J]. J Immunol, 2000, 164(4):1814-1819.
[15] Zenewicz LA, Flavell RA. Recent advances in IL-22 biology[J]. Int Immunol, 2011, 23(3):159-163.
[16] Witte E, Witte K, Warszawska K, et al. Interleukin-22: a cytokine produced by T, NK and NKT cell subsets, with importance in the innate immune defense and tissue protection[J]. Cytokine Growth Factor Rev, 2010, 21(5): 365-379.
[17] Xie MH, Aggarwal S, Ho WH, et al. Interleukin(IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R[J]. J Biol Chem, 2000, 275(40): 31335-31339.
[18] Akil H, Abbaci A, Lalloue F, et al. IL22/IL-22R pathway induces cell survival in human glioblastoma cells[J]. PLoS One, 2015, 10(3): e0119872. doi: 10.1371/journal.pone.0119872.
[1] 董晓婧,李静,赵蕾,齐眉,孙泽雨,王红,赵蔚明. 鼠衣原体肺部感染中自然杀伤细胞对白细胞介素-22产生及疾病进程的影响[J]. 山东大学学报（医学版）, 2016, 54(11): 19-23.
[2] 田馨莉, 矫俊, 张腾, 马道新, 崔保霞. Th22细胞联合Th17细胞在宫颈癌外周血中的表达及意义[J]. 山东大学学报（医学版）, 2015, 53(7): 43-47.
[3] 白金霞, 白金运, 石秀菊, 许洪伟. IL-22介导的STAT3信号通路对急性重症胰腺炎小鼠的保护作用[J]. 山东大学学报（医学版）, 2015, 53(6): 1-6.
[4] 张荣军, 韩波, 高聆, 朱梅, 丁国玉, 梁燕. siRNA沉默CD40基因对实验性自身免疫性心肌炎大鼠的作用及IL-22表达的影响[J]. 山东大学学报（医学版）, 2015, 53(5): 36-40.
[5] 范恒建，张玉可，肖伟，张一，李海军，王得翔 . 肺腺癌患者外周血Treg和Th17细胞的变化及其对预后的影响[J]. 山东大学学报(医学版), 2012, 50(9): 73-78.
[6] 郑鲲鹏1，宋辉2，樊明德1，张源3，李科1，王成伟1. h17细胞相关因子在人脑胶质瘤中的测定[J]. 山东大学学报(医学版), 2012, 50(6): 87-91.
[7] 尹萌，戚向敏，颜士果，魏美荣，陈丹，宋京，张达. Th17细胞在口腔扁平苔藓中的作用[J]. 山东大学学报(医学版), 2012, 50(12): 114-.
[8] 刘香岚,姜杨,王群,朱法良,董兆静,郭春,张利宁. PDCD4 基因对T淋巴细胞亚群分化的影响[J]. 山东大学学报(医学版), 2012, 50(11): 6-.
[9] 陈璇，王玉. Vogt-小柳原田病患者外周血Th17细胞的变化及意义[J]. 山东大学学报(医学版), 2011, 49(9): 153-.
[10] 李海军，肖伟. 肺腺癌患者外周血Th17细胞比例变化及临床意义[J]. 山东大学学报(医学版), 2011, 49(8): 108-112.
[11] 李娜，边红军，张娟，季晓平. 慢性心力衰竭患者外周血中Th17及Treg细胞的检测及意义[J]. 山东大学学报(医学版), 2010, 48(6): 100-103.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发