山东大学学报(医学版) ›› 2015, Vol. 53 ›› Issue (7): 48-52.doi: 10.6040/j.issn.1671-7554.0.2015.184
张晓晖, 颜磊, 齐莎莎, 路真真, 李明江, 赵兴波
ZHANG Xiaohui, YAN Lei, QI Shasha, LU Zhenzhen, LI Mingjiang, ZHAO Xingbo
摘要: 目的 检测上皮-间质转化(EMT)在子宫内膜腺癌中的发生, 并探讨miR200a/E盒结合锌指蛋白1(ZEB1)信号通路在其中发挥的作用。方法 ① 分组:取自石蜡切片的子宫内膜腺癌为A组(n=45), 正常子宫内膜为B组(n=22);取自手术中的子宫内膜腺癌标本为C组(n=34), 正常子宫内膜标本为D组(n=15);② 采用免疫组化法检测A、B两组间E钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、ZEB1的表达;采用Western blotting法检测C、D两组间上述3种蛋白的表达;③ Real-time RT-PCR法检测C、D两组间各蛋白相应mRNA及miR200a的表达。结果 ① B组与A组比较, E-cadherin表达降低(P<0.05), Vimentin及ZEB1表达升高(P<0.05);② D组与C组比较, E-cadherin蛋白及mRNA表达降低(P<0.05), Vimentin蛋白及mRNA表达升高(P<0.05), ZEB1蛋白及mRNA表达升高(P<0.05);③ 与C组数据相比, D组中miR200a表达量降低(P<0.05), D组中miR200a与E-cadherin的mRNA表达量之间存在正相关关系(r=0.63, P<0.01)。结论 子宫内膜腺癌中明确发生了EMT过程, 而miR200a/ZEB1信号通路可对EMT过程中重要的分子标记物产生抑制作用。
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