您的位置:山东大学 -> 科技期刊社 -> 《山东大学学报(医学版)》

山东大学学报(医学版)

• 临床医学 • 上一篇    下一篇

精子相关抗原9在宫颈癌及宫颈上皮内瘤变中的表达及意义

张璐,颜磊,张辉,张晓晖,李明江,赵兴波   

  1. 山东大学附属省立医院妇产科,山东 济南 250021
  • 收稿日期:2014-02-10 出版日期:2014-06-10 发布日期:2014-06-10
  • 通讯作者: 赵兴波。E-mail:zxb0626@126.com
  • 基金资助:
    国家自然科学基金(81202057);山东省自然科学基金(ZR2010HM005)

Expression and clinical significance of sperm-associated antigen 9 in patients with cervical cancer and cervical intraepithelial neoplasia

ZHANG Lu, YAN Lei, ZHANG Hui, ZHANG Xiaohui, LI Mingjiang, ZHAO Xingbo   

  1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University,
    Jinan 250021, Shandong, China
  • Received:2014-02-10 Online:2014-06-10 Published:2014-06-10

摘要: 目的  检测精子相关抗原9(SPAG9)在宫颈癌、宫颈上皮内瘤变(CIN)组织中的表达及意义。方法  通过免疫组织化学、Western blotting、RT-PCR方法  检测正常宫颈组织(对照组,n=20)、CIN组织(CIN组,n=35)及宫颈癌组织(宫颈癌组,n=40)中SPAG9的表达。应用酶联免疫吸附试验(ELISA)测定健康女性、子宫肌瘤患者、CIN患者及宫颈癌患者血清标本中SPAG9浓度。结果  ① SPAG9的表达:宫颈癌组>CIN组>对照组,各组间差异有统计学意义(P均<0.05);② SPAG9 mRNA和蛋白在宫颈癌组中高表达,在对照组中无表达(P<0.05);③ 宫颈癌患者的SPAG9血清浓度高于CIN患者、子宫肌瘤患者及健康女性的SPAG9血清浓度(P<0.05),且患者术后的SPAG9血清浓度下降。结论  SPAG9在宫颈癌和CIN组织以及患者血清中表达量均增高,其作为一个新的辅助生物学指标,可为宫颈癌及CIN的早期诊断、术后检测及随诊治疗提供指导意义。

关键词: 宫颈癌, 精子相关抗原9, 宫颈上皮内瘤变, 诊断

Abstract: Objective  To investigate the clinical significance of sperm-associated antigen 9 (SPAG9) in cervical cancer and cervical intraepithelial neoplasia (CIN) patients. Methods  The expressions of SPAG9 mRNA and protein were evaluated in cervical carcinoma (cervical carcinoma group, n=40), CIN (CIN group, n=35) and normal cervical tissues (the control group, n=20) by immunohistochemistry, RT-PCR, and Western blotting. SPAG9 concentrations in serum samples from 10 healthy women, 20 women with hysteromyoma, 40 women with CIN, 40 women with cervical carcinoma, and 20 postoperative cervical carcinoma patients were detected by enzyme-linked immunosorbent assay (ELISA).  Results  ① The expression of SPAG9 was gradually increased in the control group, CIN group and cervical carcinomas group (all P<0.05); ② A significant difference has been found between cervical carcinoma group and the control group by RT-PCR and Western blotting (P<0.05); ③ SPAG9 serum concentrations in cervical carcinoma patients was significantly higher than those in the CIN and hysteromyoma patients and the healthy women (P<0.05). Furthermore, a significant decrease of SPAG9 level was detected after operation. Conclusion  SPAG9 is positively expressed in cervical carcinoma and CIN. A high humoral response against SPAG9 suggests its key role in early detection, clinical diagnosis and postoperative monitoring of cervical carcinoma and CIN patients.

Key words: Sperm-associated antigen 9, Cervical intraepithelial neoplasia, Diagnosis, Cervical carcinoma

中图分类号: 

  • R711.75
[1] 李波波 李道堂 刘曙光 王兴武. 食管癌患者血清中DKK-1的表达[J]. 山东大学学报(医学版), 2209, 47(6): 58-61.
[2] 徐平 于国放 李霞. 不同类型甲状腺上动脉PSV对Graves病与桥本氏甲状腺炎鉴别诊断的价值[J]. 山东大学学报(医学版), 2209, 47(6): 62-64.
[3] 王欣,邢春燕,杨艳平. 血清磷酸丙酮酸水合酶检测对诊断侵袭性白念珠菌感染的临床价值[J]. 山东大学学报(医学版), 2209, 47(6): 92-94.
[4] 王凯,沈媛媛,程宪永,李彦敏,史宁. 嗜酸性粒细胞性食管炎1例并文献复习[J]. 山东大学学报 (医学版), 2020, 1(9): 119-121.
[5] 尹义龙,袭肖明,孟宪静. 阿尔兹海默病的智能诊断方法[J]. 山东大学学报 (医学版), 2020, 1(8): 14-21.
[6] 肖娟,肖强,丛伟,李婷,丁守銮,张媛,邵纯纯,吴梅,刘佳宁,贾红英. 两种甲状腺超声数据报告系统诊断效能的比较[J]. 山东大学学报 (医学版), 2020, 1(7): 53-59.
[7] 路璐,孙志钢,张楠. 继发性嗜血细胞综合征1例[J]. 山东大学学报 (医学版), 2020, 1(7): 122-124.
[8] 冯致余,杨彦亮,郭锐,徐惠惠,张健,郭凯,王广震,王辉. 肺动脉吊带合并气管狭窄的外科治疗[J]. 山东大学学报(医学版), 2017, 55(8): 48-51.
[9] 刘京康,杨建勇,孟丽华,姜洁. 血清miR-17-92簇在HPV阳性宫颈癌中的早期诊断价值[J]. 山东大学学报(医学版), 2017, 55(5): 86-90.
[10] 孙泽雨,陈颖,林家香,刘娟,赵蔚明. 磷脂酰肌醇-4-磷酸酶II型对宫颈癌细胞增殖及凋亡的影响[J]. 山东大学学报(医学版), 2017, 55(11): 1-6.
[11] 杜怡峰,井然. 阿尔茨海默病的诊断与治疗[J]. 山东大学学报(医学版), 2017, 55(10): 7-13.
[12] 唐毅. 阿尔茨海默病诊断标准:从临床诊断到病理生理诊断[J]. 山东大学学报(医学版), 2017, 55(10): 14-20.
[13] 李昀,仲万霞,姚宁,胡双纲,刘洪卯. 女性年龄对胚胎植入前遗传学诊断及筛查结局的影响[J]. 山东大学学报(医学版), 2017, 55(1): 60-62.
[14] 范凤景,张广英,曹淑娟,丁红宇,刘德泉. 声触诊组织量化技术在乳腺肿块定性诊断中的价值[J]. 山东大学学报(医学版), 2016, 54(9): 48-52.
[15] 左常婷, 连岩. 凶险性前置胎盘诊治现状[J]. 山东大学学报(医学版), 2016, 54(9): 1-6.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!