血清循环miR-375检测对宫颈癌早期诊断及术后监测的意义

doi:10.6040/j.issn.1671-7554.0.2015.709

摘要/Abstract

摘要： 目的检测循环miR-375在宫颈癌及宫颈上皮内瘤变(CIN)患者血清中的表达水平,探讨其对宫颈癌早期诊断及术后监测等的临床意义。方法采用实时荧光定量PCR(RT-qPCR)法分别检测64例宫颈癌患者(宫颈癌组)、64例CIN患者(CIN组)及50例健康对照组血清中循环miR-375表达水平,同时检测血清糖抗原125(CA125)水平,分析二者对宫颈癌早期诊断、术后监测的意义及血清循环miR-375与宫颈癌临床病理参数间的关系。结果宫颈癌组血清循环miR-375表达水平明显低于健康对照组和CIN组(P均<0.05);同时CIN组血清循环miR-375表达水平也明显低于健康对照组(P<0.05)。受试者工作曲线(ROC曲线)分析显示,血清循环miR-375诊断宫颈癌的曲线下面积(AUC)为0.782(95%CI:0.714,0.840),灵敏度为73.4%,特异度为78.9%, 其诊断价值高于CA125的0.677(95%CI:0.603,0.745),P<0.05。相比于宫颈癌术前,血清循环miR-375的表达水平在术后显著升高(P<0.05)。宫颈癌组患者血清循环miR-375表达水平与FIGO分期及局部淋巴结转移密切相关(P均<0.05),而与患者年龄、组织形态及细胞分化无关(P均>0.05)。结论血清循环miR-375表达水平检测有助于宫颈癌及癌前病变的早期发现及术后监测,并且可以作为宫颈癌病情进展以及淋巴结转移的辅助判断指标。

关键词: 宫颈肿瘤, 糖抗原125, 宫颈癌上皮内瘤变, miR-375

Abstract: Objective To evaluate the clinical significance of serum miR-375 in the diagnosis and postoperative monitoring of cervical cancer by analyzing its expression in cervical intraepithelial neoplasia(CIN)and cervical cancer. Methods A total of 178 subjects, including 64 patients with cervical cancer, 64 patients with CIN and 50 healthy controls, were enrolled in the study. The expressions of serum miR-375 and CA125 were measured, and their diagnostic significance was evaluated. The relationship between miR-375 and clinical parameters of cervical cancer was analyzed. Results The expression of serum miR-375 in patients with cervical cancer was significantly lower than that in patients with CIN and healthy controls(both P<0.05). The level of serum miR-375 in CIN patients was significantly lower than that in healthy controls(P<0.05). Receiver operating characteristic(ROC)curve analysis showed that the area under the ROC curve(AUC)for serum miR-375 was 0.782(95% CI: 0.714-0.840; sensitivity, 73.4%; specificity, 78.9%), which was significantly higher than that of CA125［0.677, 95%CI: 0.603-0.745］, P<0.05. The expression of serum miR-375 was significantly up-regulated after surgery in the same subset of patients with cervical cancer(P<0.05). Moreover, serum miR-375 in patients with cervical cancer was significantly correlated with FIGO stages and lymphoid node metastasis(both P<0.05), while there was no significant association between serum miR-375 and 山东大学学报 (医学版)54卷3期 -彭慧,等.血清循环miR-375检测对宫颈癌早期诊断及术后监测的意义 \=-age, histological type or cell differentiation(all P>0.05). Conclusion Serum miR-375 is conducive in the diagnosis of cervical cancer and CIN. It can serve as an index for the postoperative monitoring, progression and lymphoid node metastasis evaluation of cervical cancer.

Key words: Cervical cancer, CA125, Cervical intraepithelial neoplasia, MiR-375

中图分类号:

R737.3

彭慧,何君梅,苏雪莲. 血清循环miR-375检测对宫颈癌早期诊断及术后监测的意义[J]. 山东大学学报（医学版）, 2016, 54(3): 68-71.

PENG Hui, HE Junmei, SU Xuelian. Expression of serum miR-375 and its clinical significance in the diagnosis and postoperative monitoring of cervical cancer[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 68-71.

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