罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响

doi:10.6040/j.issn.1671-7554.0.2015.315

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (3): 72-76.doi: 10.6040/j.issn.1671-7554.0.2015.315

罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响

李志爽¹,于琼¹,张春华²

1.山东大学医学院病理学教研室, 山东济南 250012;2.山东大学附属省立医院妇产科, 山东济南 250021

收稿日期:2015-03-23 出版日期:2016-03-10 发布日期:2016-03-10
通讯作者: 张春华. E-mail:15168886369@163.com E-mail:15168886369@163.com
基金资助:
山东省优秀中青年科学家科研奖励基金(2009BSB14147)

Effects of rosiglitazone and GW9662 on the expression of PPAR-γ in JEG-3 cells

LI Zhishuang¹, YU Qiong¹, ZHANG Chunhua²

1. Department of Pathology, Medical School of Shandong University, Jinan 250012, Shandong, China;
2. Department of Gynecology and Obstetrics, Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China

Received:2015-03-23 Online:2016-03-10 Published:2016-03-10

摘要/Abstract

摘要： 目的探讨罗格列酮与GW9662对人绒癌JEG-3细胞中过氧化物酶体增殖物激活受体-γ(PPAR-γ)表达的影响。方法免疫组化法测定PPAR-γ在正常绒毛组织、葡萄胎组织及绒癌组织中的阳性表达;体外培养JEG-3细胞,将JEG-3细胞分为罗格列酮组、GW9662组和对照组,分别采用Transwell实验检测不同浓度PPAR-γ的激动剂罗格列酮与其抑制剂GW9662对JEG-3细胞侵袭能力的影响,免疫细胞化学、Western blotting及实时荧光定量PCR观察PPAR-γ在细胞中表达水平的变化。结果 PPAR-γ表达于正常绒毛组织、葡萄胎及绒癌组织的细胞核,且PPAR-γ在绒癌组织中的表达明显下调,仅有少数细胞呈弱阳性染色;Transwell实验结果显示,罗格列酮组JEG-3细胞侵袭能力明显减弱,GW9662组细胞侵袭能力增强,两者与对照组比较差异有统计学意义(P<0.05);罗格列酮处理后的JEG-3细胞PPAR-γ表达量下降,GW9662组其表达量升高,且均与对照组比较差异具有统计学意义(P<0.05)。结论罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ的调节是一种负反馈调节,并通过这种调节方式影响绒癌的侵袭能力。

关键词: 过氧化物酶体增殖物激活受体-γ, 罗格列酮, 绒毛膜癌, GW9662

Abstract: Objective To investigate the effects of peroxisome proliferator-activated receptor gamma(PPAR-γ)agonist rosiglitazone and antagonist GW9662 on PPAR-γ expression and invasion ability of JEG-3 cells. Methods Immunohistochemistry method was used to detect PPAR-γ expression and distribution in normal chorionic, hydatidiform mole and choriocarcinoma tissues. Then PPAR-γ expression in rosiglitazone, GW9662 and control groups were examined respectively by Transwell assay, immunocytochemistry, Western blotting and qPCR method. Results PPAR-γ was expressed and distributed in cell nucleus of normal chorionic, hydatidiform mole and choriocarcinoma tissues, and its expression was significant down-regulated in choriocarcinoma tissues. Invasion ability of JEG-3 cells treated by rosiglitazone was decreased, while that of GW9662 group was increased. There was a statistical significance between the two groups(P<0.05). The expression of PPAR-γ in rosiglitazone group was lower than that in GW9662 group(P<0.05). Conclusion The activity of PPAR-γ is modulated via a negative feedback regulation mechanism by rosiglitazone and GW9662, and can influence the invasion ability of choriocarcinoma cells.

Key words: Peroxisome proliferator-activated receptor gamma, Choriocarcinoma, Rosiglitazone, GW9662

中图分类号:

R737.33

李志爽,于琼,张春华. 罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响[J]. 山东大学学报（医学版）, 2016, 54(3): 72-76.

LI Zhishuang, YU Qiong, ZHANG Chunhua. Effects of rosiglitazone and GW9662 on the expression of PPAR-γ in JEG-3 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 72-76.

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罗格列酮与GW9662对人绒癌JEG-3细胞中PPAR-γ表达的影响

Effects of rosiglitazone and GW9662 on the expression of PPAR-γ in JEG-3 cells

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