补充多种微量元素对高糖饮食大鼠的保护作用

doi:10.6040/j.issn.1671-7554.0.2022.1258

摘要/Abstract

摘要： 目的观察联合补充多种微量元素对高糖饮食大鼠抗炎和抗氧化功能的影响。方法 40只雄性Wistar大鼠随机分为空白组、微量元素(TE)组、高糖饮食(HSD)组和高糖饮食+微量元素(HSD+TE)组,每组10只。喂养的同时,每天通过灌胃给予各组大鼠生理盐水或11种微量元素混合溶液(硼、钒、铬、锰、铁、钴、铜、锌、硒、锶和钼),20周后评估大鼠体质量、组织质量、血清葡萄糖含量、氧化应激和炎症标志物的变化。结果高糖饮食导致大鼠体质量和血糖水平明显增加(P<0.05),并显著引起氧化应激和炎症反应(P<0.05)。此外,微量元素的补充显著抑制由于微量元素缺乏和高糖饮食诱发的白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的升高(P<0.05),但促进了白细胞介素-4(IL-4)和白细胞介素-10(IL-10)的分泌(P<0.05)。其次,补充微量元素抑制高糖饮食大鼠血清及脾脏过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性的下降(P<0.05),减少丙二醛(MDA)的产生(P<0.05),并提高高糖饮食大鼠血清谷胱甘肽还原酶(GR)和总抗氧化能力(T-AOC)(P<0.05)。维持微量元素的平衡可以防止高糖饮食引起的大鼠体质量增加(P<0.05)。结论对高糖饮食大鼠同时补充多种微量元素对预防糖尿病有较好的效果,可有效控制血糖水平的升高,增强抗氧化能力,减轻炎症反应,预防高糖饮食引起大鼠体质量上升。

关键词: 微量元素, 高糖饮食, 氧化应激, 炎症细胞因子, 大鼠

Abstract: Objective To observe the effects of supplementation of multiple trace elements on the anti-inflammatory and antioxidant functions in rats with high-sucrose diet. Methods A total of 40 male Wistar rats were randomly divided into blank group, trace elements(TE)group, high-sucrose diet(HSD)group and high-sucrose diet + trace elements(HSD+TE)group, with 10 rats in each group. Normal saline or a mixture of 11 trace elements(B, V, Cr, Mn, Fe, Co, Cu, Zn, Se, Sr and Mo)was given daily by gavage to the rats. After 20 weeks, the changes of body weight, tissue weight, serum glucose content, oxidative stress and inflammatory markers were measured. Results High-sucrose diet induced a noticeable increase in body weight and serum glucose content(P<0.05), and significantly caused oxidative stress and inflammation(P<0.05). In addition, the supplementation of trace elements significantly inhibited the increase of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)induced by trace element deficiency and high-sucrose diet(P<0.05), but promoted the secretion of interleukin-4(IL-4)and interleukin-10(IL-10)(P<0.05). The supplementation of trace elements inhibited the decrease of the activities of catalase(CAT), total superoxide dismutase(T-SOD)and glutathione peroxidase(GSH-Px)in serum and spleen(P<0.05), reduced the production of malondialdehyde(MDA)(P<0.05), and increased serum glutathione reductase(GR)and total antioxidant capacity(T-AOC)in serum of rats with high-sucrose diet(P<0.05). Maintaining the balance of trace elements prevented weight gain caused by high-sucrose diet(P<0.05). Conclusion The supplementation of multiple trace elements to rats on a high-sucrose diet has a good effect on the prevention of diabetes, which can effectively control the increase of blood glucose level, enhance the antioxidant capacity, reduce the inflammatory response and prevent the weight gain.

Key words: Trace elements, High-sucrose diet, Oxidative stress, Inflammatory factor, Rat

中图分类号:

R151

祁少俊,唐延金,张正铎,吴虹,张佳程,秦川,刘锐,高希宝. 补充多种微量元素对高糖饮食大鼠的保护作用[J]. 山东大学学报 (医学版), 2023, 61(7): 19-26.

QI Shaojun, TANG Yanjin, ZHANG Zhengduo, WU Hong, ZHANG Jiacheng, QIN Chuan, LIU Rui, GAO Xibao. Protective effects of supplementing various trace elements on rats with high-sucrose diet[J]. Journal of Shandong University (Health Sciences), 2023, 61(7): 19-26.

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