30名健康志愿者饮用饮久舒对肝代谢酶的影响

doi:10.6040/j.issn.1671-7554.0.2020.1633

摘要/Abstract

摘要： 目的探讨饮久舒植物饮品对肝脏代谢酶的影响。方法选取30名健康志愿者,在饮用饮久舒前及饮用后30、60、120 min时各抽取5 mL静脉血,分离血浆,采用酶联免疫吸附法(ELISA)检测血浆中乙醇脱氢酶(ADH)、乙醛脱氢酶(ALDH)、还原型谷胱甘肽(GSH)、谷胱甘肽巯基转移酶P1(GSTP1)、谷胱甘肽巯基转移酶M3(GSTM3)的水平。结果 30名健康志愿者饮用饮久舒后,血浆中ADH、ALDH、GSH、GSTP1、GSTM3五种酶含量均呈升高趋势。与饮用前水平相比,ADH在饮用30 min后组间差异达到有统计学意义的水平(P=0.014),ALDH在饮用120 min后组间差异达到有统计学意义的水平(P=0.016),GSH在饮用60 min后组间差异达到有统计学意义的水平(P=0.031),GSTP1在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.046, P=0.012),GSTM3在饮用30 min和60 min后组间差异达到有统计学意义的水平(P=0.008, P=0.039)。结论饮久舒能提高乙醇代谢相关酶和抗氧化物质的水平,可能是其发挥解酒护肝作用的潜在机制。

关键词: 饮久舒, 乙醇脱氢酶, 乙醛脱氢酶, 还原型谷胱甘肽, 氧化应激

Abstract: Objective To explore the effects of a plant beverage, Yin-Jiu-Shu, on liver metabolizing enzymes. Methods A total of 30 healthy volunteers were enrolled. Before, 30 min, 60 min and 120 min after Yin-Jiu-Shu were administered, 5 mL pf venous blood was collected, and plasma was immediately separated to detect the levels of alcohol dehydrogenase(ADH), acetaldehyde dehydrogenase(ALDH), glutathione(GSH), glutathione S-transferase P1(GSTP1), and glutathione S-transferase M3(GSTM3)using enzyme-linked immunosorbent assay(ELISA). Results After Yin-Jiu-Shu was administered, the plasma levels of ADH, ALDH, GSH, GSTP1, GSTM3 significantly increased at 30 minute point(P=0.014), 120 minute point (P=0.016), 60 minute point(P=0.031), 30 and 60 minute points (P=0.046, P=0.012), 30 and 60 minute points(P=0.008, P=0.039), respectively. Conclusion Yin-Jiu-Shu can improve the levels of alcohol metabolism related enzymes and antioxidants, which might be its underlying mechanism to promote alcohol metabolism and protect liver.

Key words: Yin-Jiu-Shu, Alcohol dehydrogenase, Acetaldehyde dehydrogenase, Glutathione, Oxidative stress

中图分类号:

R575

向琳,陈腊梅,王婧雯,李海铭,李浩宇,王菊,范玉琛,王凯. 30名健康志愿者饮用饮久舒对肝代谢酶的影响[J]. 山东大学学报 (医学版), 2021, 59(3): 81-85.

XIANG Lin, CHEN Lamei, WANG Jingwen, LI Haiming, LI Haoyu, WANG Ju, FAN Yuchen, WANG Kai. Effects of drinking Yin-Jiu-Shu on liver metabolizing enzymes in 30 healthy volunteers[J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 81-85.

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