TOLL样受体4在15例子宫腺肌病中的表达及作用

doi:10.6040/j.issn.1671-7554.0.2019.1452

摘要/Abstract

摘要： 目的探讨TOLL样受体4(TLR4)在子宫腺肌病发病中的作用及其与凋亡的关系。方法 Western blotting检测TLR4蛋白在子宫腺肌病患者内膜组织和对照组正常子宫内膜中表达水平;Real-time PCR检测TLR4 mRNA在子宫腺肌病患者子宫内膜组织和对照组正常子宫内膜中表达水平;免疫组织化学染色法检测子宫腺肌病患者子宫内膜组织和对照组正常子宫内膜中TLR4的表达及定位情况;TUNEL检测子宫腺肌病患者子宫内膜组织和对照组正常子宫内膜的细胞凋亡水平;通过脂多糖(LPS)激活h-ESC细胞系,使细胞系中的TLR4的表达升高,流式细胞术检测凋亡水平,由此验证TLR4对细胞凋亡的作用。结果 Western blotting 结果显示TLR4的蛋白表达水平在子宫腺肌病子宫内膜组织中较对照组降低(P=0.0182),Real-time PCR结果显示TLR4的mRNA表达水平在子宫腺肌病子宫内膜组织中较对照组降低(P=0.0197),免疫组织化学染色法结果显示TLR4主要位于子宫内膜组织和对照组正常子宫内膜中的腺上皮细胞质中表达,且子宫腺肌病患者子宫内膜组织中TLR4的表达水平较对照组降低(P=0.0160)。TUNEL结果显示子宫腺肌病内膜细胞中细胞凋亡较对照组降低(P=0.0024),h-ESC细胞系中TLR4被成功激活后,流式细胞术显示右下象限细胞所占比例升高,表明细胞凋亡水平升高。结论 TLR4可能通过调控细胞凋亡在子宫腺肌病的发生发展中发挥作用。

关键词: 子宫腺肌病, 内膜, TLR4, 凋亡

Abstract: Objective To explored the role of toll-like receptor 4(TLR4)in the pathogenesis of adenomyosis(AS)and the relationship with apoptosis. Methods The protein and mRNA expressions and distribution of TLR4 in the endometrium of AS patients and normal controls were detected with Western blotting, real-time PCR and immunohistochemistry, respectively. The cell apoptosis was determined with TUNEL. After the expression of LTR4 in h-ESC cell line was activated with lipopolysaccharide(LPS), the level of apoptosis was detected with flow cytometry in order to evaluate the effect of TLR4 on apoptosis. Results The protein expression( P=0.0128)and mRNA expression(P=0.0197) of TLR4 were lower in AS endometrium than in normal endometrium. TLR4 mainly distributed in the glandular epithelial cytoplasm in endometrium, and TLR4 expression was lower in AS endometrium than in normal endometrium (P=0.0160). The apoptosis level was lower in AS endometrium than in normal endometrium (P=0.0024). After TLR4 was activated in h-ESC cell line, the number of cells in the lower right quadrant increased, indicating elevated apoptosis. Conclusion TLR4 plays an important role in adenomyosis by regulating apoptosis.

Key words: Adenomyosis, Endometrium, TLR4, Apoptosis

中图分类号:

R711.71

王冰玉,杨阳,晁岚. TOLL样受体4在15例子宫腺肌病中的表达及作用[J]. 山东大学学报 (医学版), 2020, 58(6): 47-52.

WANG Bingyu, YANG Yang, CHAO Lan. Expression and role of toll-like receptor 4 in adenomyosis[J]. Journal of Shandong University (Health Sciences), 2020, 58(6): 47-52.

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