山东大学学报 (医学版) ›› 2017, Vol. 55 ›› Issue (12): 18-23.doi: 10.6040/j.issn.1671-7554.0.2017.658
宫晓丹1,2,赵方正2,3,曹可1,2,邓鹏辉2,张才擎2
GONG Xiaodan1,2, ZHAO Fangzheng2,3, CAO Ke1,2, DENG Penghui2, ZHANG Caiqing2
摘要: 目的 研究肌球蛋白轻链激酶(MLCK)在哮喘小鼠气道中的表达及其对哮喘气道炎症和肺功能的影响。 方法 将20只雌性BALB/c小鼠随机分为对照组(n=10)及哮喘组(n=10),利用鸡卵白蛋白(OVA)建立小鼠哮喘模型。光学显微镜下计数血清中白细胞及嗜酸性粒细胞总数;ELISA方法检测肺泡灌洗液中炎性介质IL-4、IFN-γ水平; Western blotting法检测小鼠肺组织MLCK的表达;免疫实时荧光定量(qPCR)检测MLCK在基因水平的表达。 结果 肺功能检测示哮喘组小鼠最大呼气流量峰值(PEF)、第0.4秒用力呼气容积占用力肺活量比值(FEV0.4/FVC)均低于对照组(P均<0.001);哮喘组小鼠白细胞及嗜酸性粒细胞计数均高于对照组(P均<0.001);ELISA结果示哮喘组小鼠BALF中炎性介质IL-4浓度较对照组明显升高(P<0.001),而IFN-γ浓度较对照组降低(P<0.001);哮喘组小鼠肺组织MLCK表达量明显升高(P=0.006);与对照组相比,哮喘组MLCK在基因水平上的表达升高(P<0.001)。 结论 MLCK可能参与哮喘气道炎症反应并影响肺功能。
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