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山东大学学报 (医学版) ›› 2017, Vol. 55 ›› Issue (12): 18-23.doi: 10.6040/j.issn.1671-7554.0.2017.658

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肌球蛋白轻链激酶对哮喘小鼠气道炎症及肺功能的影响

宫晓丹1,2,赵方正2,3,曹可1,2,邓鹏辉2,张才擎2   

  1. 1. 山东大学临床医学院, 山东 济南 250012;2. 山东大学附属千佛山医院呼吸内科, 山东 济南 250014;3. 山东中医药大学第二附属医院, 山东 济南 250001
  • 出版日期:2017-12-20 发布日期:2022-09-27
  • 通讯作者: 张才擎. E-mail:freezcq66@163.com

Effect of myosin light chain kinase on airway inflammation and lung function in asthma model mice

GONG Xiaodan1,2, ZHAO Fangzheng2,3, CAO Ke1,2, DENG Penghui2, ZHANG Caiqing2   

  1. 1. Medical College of Shandong University, Jinan 250012, Shandong, China;
    2. Respiratory Physicians of Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

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摘要： 目的 研究肌球蛋白轻链激酶(MLCK)在哮喘小鼠气道中的表达及其对哮喘气道炎症和肺功能的影响。 方法 将20只雌性BALB/c小鼠随机分为对照组(n=10)及哮喘组(n=10),利用鸡卵白蛋白(OVA)建立小鼠哮喘模型。光学显微镜下计数血清中白细胞及嗜酸性粒细胞总数;ELISA方法检测肺泡灌洗液中炎性介质IL-4、IFN-γ水平; Western blotting法检测小鼠肺组织MLCK的表达;免疫实时荧光定量(qPCR)检测MLCK在基因水平的表达。 结果 肺功能检测示哮喘组小鼠最大呼气流量峰值(PEF)、第0.4秒用力呼气容积占用力肺活量比值(FEV0.4/FVC)均低于对照组(P均<0.001);哮喘组小鼠白细胞及嗜酸性粒细胞计数均高于对照组(P均<0.001);ELISA结果示哮喘组小鼠BALF中炎性介质IL-4浓度较对照组明显升高(P<0.001),而IFN-γ浓度较对照组降低(P<0.001);哮喘组小鼠肺组织MLCK表达量明显升高(P=0.006);与对照组相比,哮喘组MLCK在基因水平上的表达升高(P<0.001)。 结论 MLCK可能参与哮喘气道炎症反应并影响肺功能。

关键词: 哮喘, 肌球蛋白轻链激酶, 肺功能, IL-4, INF-γ

Abstract: Objective To study the effect of myosin light chain kinase(MLCK)on airway inflammation and pulmonary function through the determination of its expression in asthmatic mice airway. Methods Twenty female BALB/c mice were randomly divided into normal control group(n=10)and asthma group(n=10). Mice model of asthma was established by using chicken ovalbumin(OVA). The counts of leukocytes and eosinophilic granulocytes were observed with optical microscope. The expressions of the inflammatory mediators as IL-4 and IFN-γ in bronchoalveolar lavage fluid(BALF)were detected by ELISA kit. The expression of MLCK in the lung tissue of asthmatic mice was detected by Western blotting. The expression of MLCK in the gene level was detected by qPCR. Results The lung function test showed that peak expiratory flow(PEF), the ratio of forced expiratory volume in 0.4 second to forced vital capacity(FEV0.4/FVC)in asthma group was lower than those in normal control group(P<0.001). The counts of leukocytes and eosinophilic granulocytes in asthma group were higher than those in normal control group(P<0.001). The IL-4 level in the BALF in asthma group was higher than that in normal control group(P<0.001), while the IFN-γ level 山 东 大 学 学 报 (医 学 版)55卷12期 -宫晓丹,等.肌球蛋白轻链激酶对哮喘小鼠气道炎症及肺功能的影响 \=-was lower than that in nornal control group(P<0.001). The expression of MLCK in the lung tissue of asthma group was significantly increased(P=0.006). The expression of MLCK in the gene level of asthma group was higher compared with the normal control group(P<0.001). Conclusion MLCK may be involved in the airway inflammation response and influence the lung function.

Key words: Asthma, Myosin light chain kinase, Lung function, IL-4, IFN-γ

中图分类号: 

  • R562.2+5
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