山东大学学报 (医学版) ›› 2024, Vol. 62 ›› Issue (8): 107-116.doi: 10.6040/j.issn.1671-7554.0.2024.0576
• • 上一篇
郝晓梦1,于泽源2,张晓芳1,2
HAO Xiaomeng1, YU Zeyuan2, ZHANG Xiaofang1,2
摘要: 目的 探讨肺胃型黏液腺癌(gastric mucinous adenocarcinoma, GMA)的形态特征、临床病理特点、基因改变及临床预后,为肺黏液腺癌的个体化治疗提供帮助。 方法 回顾性分析肺浸润性非黏液腺癌(invasive non-mucinous lung adenocarcinoma, INMA)48例、浸润性黏液腺癌(invasive mucinous adenocarcinoma, IMA)40例的资料,其中IMA包括单纯性黏液腺癌(simple invasive mucinous adenocarcinoma, SIMA)30例及黏液-非黏液混合型腺癌(mucinous and non-mucinous mixed adenocarcinoma, MNMA)10例,SIMA根据形态进一步分为GMA、柱状细胞型黏液腺癌(colunmar cell mucinous adenocarcinoma, CMA)及混合型单纯性黏液腺癌(mixed simple invasive mucinous adenocarcinoma, MSIMA)。收集患者的临床病理资料。对所有石蜡样本行免疫组织化学(immunohistochemistry, IHC)检测人黏蛋白5ac(mucin 5ac, MUC5ac)、人黏蛋白6(mucin 6, MUC6)、甲状腺转录因子-1(thyroid transcription factor-1, TTF-1)、细胞角蛋白7(cytokeratin 7, CK7)、细胞角蛋白5/6(cytokeratin 5/6, CK5/6)等指标,并对所有样本行聚合酶链式反应(polymerase chain reaction, PCR)或二代测序(next-generation sequencing, NGS)检测肺癌相关驱动基因。 结果 GMA的肿瘤平均直径显著小于CMA、MSIMA、MNMA及INMA中的肿瘤平均直径,发病年龄略高于其他类型腺癌,且好发于肺下叶。MUC6在GMA中的阳性率为92.93%,显著高于在CMA(8.33%)、MNMA(20.00%)及INMA(0%)中的阳性率,差异有统计学意义(P<0.001);而TTF-1和CK7在GMA中的表达率显著低于在MNMA及INMA中的表达率(P均<0.001)。14例GMA中,10例伴有KRAS基因点突变,比例显著高于CMA、MNMA及INMA。且MUC6的表达与KRAS基因突变呈显著正相关(Pearson 相关系数=0.590)。 结论 GMA直径较小,好发于肺下叶,高表达 MUC6,且KRAS基因突变率高,具有独特的病理、临床及分子特点,应作为独立类型。
中图分类号:
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