白头翁汤对短肠综合征小鼠肠道功能的影响

doi:10.6040/j.issn.1671-7554.0.2023.0309

摘要/Abstract

摘要： 目的探讨白头翁汤对改善短肠综合征(SBS)小鼠肠道通透性和肠道菌群结构的影响。方法采用随机数字表法将昆明小鼠平均分为假手术组、模型组、白头翁汤高剂量组、白头翁汤中剂量组、白头翁汤低剂量组。假手术组进行空肠横断吻合,其余组均切除中段小肠,建立SBS模型。假手术组和模型组术后第2天开始,灌胃生理盐水,其余白头翁汤组分别灌胃等体积不同浓度白头翁汤。持续治疗4周后处死小鼠,HE染色观察回肠组织病理形态;免疫组化测定回肠组织咬合蛋白(Occludin)和闭锁小带蛋白-1(ZO-1)蛋白表达水平;ELISA检测血清肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)含量;16S rDNA测序技术分析小鼠肠道菌群结构。结果与SBS模型组相比,白头翁汤小鼠回肠黏膜炎性细胞浸润呈剂量依赖性减少,回肠绒毛高度、环状肌厚度和纵肌厚度均明显增加,隐窝深度明显降低(P<0.05)。回肠Occludin和ZO-1蛋白表达水平明显增加(P<0.01),血清TNF-α和IL-6水平明显下降(P<0.01)。肠道菌群分析显示,白头翁汤可显著调节门和属的相对丰度。结论白头翁汤可改善SBS小鼠肠黏膜结构,修复肠黏膜屏障,调节菌群相对丰度,增加肠道菌群多样性。

关键词: 白头翁汤, 短肠综合征, 肠道通透性, 肠道菌群

Abstract: Objective To explore the effects of Pulsatilla decoction(BTWD)on the intestinal permeability and intestinal flora structure in mice with short bowel syndrome(SBS). Methods Kunming mice were randomly divided into the sham group, model group, high-dose group, medium-dose group, and low-dose group. Mice in the sham group underwent jejunal transection anastomosis, while in the other groups, the middle small intestine of mice were resected to establish the SBS models. In the sham and model groups, saline was instilled by gavage starting from the 2nd postoperative day. In the other groups, equal volume of BTWD with different concentrations was instilled by gavage. After 4 weeks of treatment, the mice were sacrificed. The pathological morphology of ileal tissue was observed with HE staining; the protein expressions of occludin and ZO-1 were determined with immunohistochemistry; the levels of serum tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6)were detected with ELISA; the intestinal flora was analyzed with 16S rDNA sequencing. Results Compared with the model group, the high-, medium-, and low-dose groups showed dose-dependently reduced ileal mucosal inflammatory cell infiltration; the ileal villus height, circular muscle thickness and longitudinal muscle thickness were significantly increased; the crypt depth was significantly reduced(P<0.05); the protein expressions of ileal occludin and ZO-1 were significantly increased(P<0.01); the serum TNF-α and IL-6 levels were significantly decreased(P<0.01). Analysis of intestinal flora showed that BTWD significantly regulated the relative abundance of phylum and genus. Conclusion BTWD can improve the intestinal mucosal structure, repair the intestinal mucosal barrier, regulate the relative abundance of phyla, and increase the diversity of intestinal flora.

Key words: Pulsatilla decoction, Short bowel syndrome, Intestinal permeability, Intestinal flora

中图分类号:

R574.5

陈泰宇,唐学贵,唐诗宇,张智彬,陈思敏,李敏,庞峻. 白头翁汤对短肠综合征小鼠肠道功能的影响[J]. 山东大学学报 (医学版), 2023, 61(9): 29-37.

CHEN Taiyu, TANG Xuegui, TANG Shiyu, ZHANG Zhibin, CHEN Simin, LI Min, PANG Jun. Effects of Pulsatilla decoction on the intestinal function in mice with short bowel syndrome[J]. Journal of Shandong University (Health Sciences), 2023, 61(9): 29-37.

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