山东大学学报(医学版) ›› 2016, Vol. 54 ›› Issue (3): 24-29.doi: 10.6040/j.issn.1671-7554.0.2015.359
赵云霞,宋静,张灿灿,林雪艳,尉蔚,田永杰
ZHAO Yunxia, SONG Jing, ZHANG Cancan, LIN Xueyan, WEI Wei, TIAN Yongjie
摘要: 目的 探讨CXXC4对卵巢癌细胞的增殖及药物敏感性的影响。 方法 采用Western blotting和Real-time PCR分别从蛋白水平和mRNA水平检测正常卵巢上皮细胞和卵巢癌细胞中的CXXC4表达,脂质体法将重组质粒pcDNA3-CXXC4和siRNA-CXXC4分别转染人卵巢癌细胞SKOV3、Caov3和正常卵巢上皮细胞IOSE80,CCK8法分析CXXC4对细胞增殖及药物敏感性的影响。 结果 与正常卵巢上皮细胞相比,卵巢癌细胞中CXXC4低表达,外源性CXXC4过表达抑制卵巢癌细胞SKOV3、Caov3的增殖能力,以及增强肿瘤细胞对化疗药物的敏感性;降低CXXC4的表达能促进正常卵巢上皮细胞的增殖能力,促进细胞对化疗药物的耐受。 结论 CXXC4的过表达抑制卵巢癌细胞的增殖能力,且增强肿瘤细胞对化疗药物的敏感性,提示其可能作为一个潜在的卵巢癌疗效预测标记物以及化疗增敏的分子靶点。
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