异丙酚对卡巴胆碱引起的肠黏膜分泌的影响

doi:10.6040/j.issn.1671-7554.0.2015.928

山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (3): 19-23.doi: 10.6040/j.issn.1671-7554.0.2015.928

异丙酚对卡巴胆碱引起的肠黏膜分泌的影响

孙华^1,2,汤树海³,王颢诒⁴,安宁⁴,许翠萍⁵,赵东波⁶

1.山东大学护理学院, 山东济南 250012;2.山东大学附属山东省肿瘤医院急诊科, 山东济南 250117;3.山东大学齐鲁医院麻醉科, 山东济南 250012;4.山东大学医学院生理系, 山东济南 250012;5.山东大学附属千佛山医院人力资源部, 山东济南 250014;6.山东大学附属山东省肿瘤医院胸外科, 山东济南 250117

收稿日期:2015-09-29 出版日期:2016-03-10 发布日期:2016-03-10
通讯作者: 赵东波. E-mail:zhdb88@126.com E-mail:zhdb88@126.com
基金资助:
山东大学基本科研业务费专项资金(2014QLKY08)。

Effect of propofol on carbachol-induced intestinal mucosa secretion

SUN Hua^1,2, TANG Shuhai³, WANG Haoyi⁴, AN Ning⁴, XU Cuiping⁵, ZHAO Dongbo⁶

1. School of Nursing, Shandong University, Jinan 250012, Shandong, China;
2. Department of Emergency, Shandong Cancer Hospital Affilicated to Shandong University, Jinan 250117, Shandong, China;
3. Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
4. Department of Physiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China;
5. Department of Human Resources, Qianfoshan Hospital Affilicated to Shandong University, Jinan 250014, Shandong, China;
6. Department of Thoracic Surgery, Shandong Cancer Hospital Affilicated to Shandong University, Jinan 250117, Shandong, China

Received:2015-09-29 Online:2016-03-10 Published:2016-03-10

摘要/Abstract

摘要： 目的观察异丙酚对卡巴胆碱(CCh)引起的肠黏膜分泌的影响。方法利用Ussing chamber技术监测黏膜短路电流(I_sc)的变化。结果 CCh引起大鼠回肠黏膜I_sc升高;异丙酚明显抑制CCh引起的大鼠回肠黏膜I_sc的变化,且呈现明显的浓度依赖效应(10^-2~10^-6mol/L),这一抑制效应仅发生在浆膜层。钠离子通道阻断剂河豚毒素及N型胆碱能受体阻断剂六甲溴铵都不能影响异丙酚引起的抑制作用。结论静脉麻醉药异丙酚可抑制CCh引起的肠黏膜分泌增加,其作用是通过抑制肠黏膜上皮基底侧的M型胆碱能受体信号途径实现。

关键词: 异丙酚, 乙酰胆碱受体, 短路电流, 卡巴胆碱, 肠分泌

Abstract: Objective To explore the effect of propofol on carbachol(CCh)-induced chloride secretion in rat ileum mucosa. Methods Cch-induced chloride secretion was measured by Ussing chamber technique. Results Propofol attenuated the CCh-stimulated increase in short-circuit current(I_sc)of rat ileum mucosa in a dose-dependent manner(10^-2-10^-6mol/L). The propofol-evoked inhibitory effect was serosal side-dependent. Furthermore, both TTX, a Na⁺ channel blocker, and hexamethonium bromide, a nicotinic acetylcholine receptor antagonist, had no effect on propofol-evoked inhibitory effect. Conclusion Propofol inhibites CCh-induced intestinal secretion via basolateral mAChR pathway.

Key words: Carbachol, Acetylcholine receptor, Propofol, Intestinal secretion, Short-circuit current

中图分类号:

R966

孙华,汤树海,王颢诒,安宁,许翠萍,赵东波. 异丙酚对卡巴胆碱引起的肠黏膜分泌的影响[J]. 山东大学学报（医学版）, 2016, 54(3): 19-23.

SUN Hua, TANG Shuhai, WANG Haoyi, AN Ning, XU Cuiping, ZHAO Dongbo. Effect of propofol on carbachol-induced intestinal mucosa secretion[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 19-23.

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异丙酚对卡巴胆碱引起的肠黏膜分泌的影响

Effect of propofol on carbachol-induced intestinal mucosa secretion

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