1型糖尿病大鼠Runx2、Osterix表达变化及唑来膦酸的干预作用

doi:10.6040/j.issn.1671-7554.0.2014.744

摘要/Abstract

摘要： 目的观察1型糖尿病大鼠血清胰岛素和骨钙素水平, 骨组织Runx2和Osterix(OSX)基因转录水平及唑来膦酸的干预作用。方法 Wistar雌性大鼠170只, 随机分为正常对照组(n=40)和糖尿病模型组(n=130)。糖尿病模型组一次性腹腔注射链脲佐菌素(60 mg/kg)制作1型糖尿病模型, 随机选取120只分为模型组、预防组和治疗组, 每组40只。预防组和治疗组分别在造模成功和造模成功2周后静脉给予唑来膦酸(0.1 mg/kg)。ELISA法检测血清胰岛素和骨钙素水平;RT-PCR法检测右侧股骨Runx2和OSX mRNA表达水平。结果模型组、预防组和治疗组与正常对照组相比, 血清胰岛素水平显著下降(P<0.05)。骨钙素水平模型组显著低于正常对照组(P<0.05),预防组高于正常对照组和模型组(P<0.05),治疗组在12周高于预防组(P<0.05)。模型组骨组织Runx2和OSX mRNA表达水平与正常对照组相比显著降低(P<0.05), 预防组和治疗组表达均明显高于正常对照组和模型组(P<0.05), 预防组表达上调程度高于治疗组。结论在1型糖尿病大鼠模型中, 骨组织Runx2和OSX mRNA表达水平显著下降;预防性应用唑来膦酸可逆转Runx2和OSX mRNA表达的下降, 促进骨形成。

关键词: Osterix, 1型糖尿病, Runx2, 唑来膦酸, 胰岛素, 骨钙素

Abstract: Objective To observe the levels of serum insulin and osteocalcin, osteoblast specific transcription factor Runx2 and Osterix (OSX) gene in type 1 diabetic rats, and the intervention effect of zoledronic acid. Methods A total of 170 Wistar rats were randomly divided into two groups:40 rats were enrolled into the control group the other animals were injected with streptozotocin (60 mg/kg) to establish type 1 diabetic models. After that, 120 rats in this group were randomly divided into the model group (n=40), prevention group (n=40) and treatment group (n=40). Rats in the prevention group and treatment group received a bolus injection of zoledronic acid (0.1 mg/kg) at the onset of diabetes and 2 weeks later, respectively. The serum INS and OC were detected with ELISA. Expressions of Runx2 and OSX mRNA in the right femur were detected with RT-PCR. Results ELISA results showed that the serum INS in the model group, prevention group and treatment group was significantly reduced compared with that in the control group (P<0.05). The serum OC was lower in the model group that in the control group (P<0.05), while higher in the prevention group than in the control group and model group (P<0.05). At week 12, serum OC was higher in the treatment group than in the prevention group (P<0.05). RT-PCR tests showed that the expressions of Runx2 andOSX mRNA were significantly lower in the model group than in the control group (P<0.05), while remarkably higher in the prevention group and treatment group than in the control group and model group (P<0.05). The upregulation was higher in the prevention group than in the model group. Conclusion Expressions of Runx2 and OSX mRNA were significantly decreased in the bone of type 1 diabetic rat models. Prophylactic use of zoledronic acid can improve the levels of serum OC, and reverse the decreasing expression of Runx2 and OSX mRNA, thus promoting bone formation.

Key words: Insulin, Osteocalcin, Runx2, Type 1 diabetes, Osterix, Zoledronic acid

中图分类号:

R589

赵旭, 崔敏, 于灵芝, 张娜, 曹鲁宁. 1型糖尿病大鼠Runx2、Osterix表达变化及唑来膦酸的干预作用[J]. 山东大学学报（医学版）, 2015, 53(3): 56-61.

ZHAO Xu, CUI Min, YU Lingzhi, ZHANG Na, CAO Luning. Changes of Runx2 and Osterix expression in type 1 diabetic rats and the intervention effect of zoledronic acid[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(3): 56-61.

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