Table of Content

  

16 August 2008
Volume 46 Issue 8

Articles


Articles

A pancreatic cancer model with high liver metastatic potential in nude mice

CUI Ya-zhou,JIA Qing,WANG Zhao-peng,WANG Zhao-xia,TIAN Mei,ZHANG Yue-ying,ZHANG Wei-dong,HAN Jin-xiang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  739-741. 

Abstract ( 1569 )   PDF (337KB) ( 385 )   Save

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Objective〓〖WTBZ〗To establish a pancreatic cancer nude mice model with high metastatic potential for study of the mechanism and drug screening of pancreatic cancer liver metastasis. 〖WTHZ〗Methods〓〖WTBZ〗Human pancreatic cancer cell lines MIA-PaCa2 were injected into the spleen, and cancer cells from liver metastatic foli of mice were cultured in vitro and subsequently injected into the spleen. After the procedures were performed three times, a sub-line of MIA-PaCa2 with high livermetastatic potential MIA-PaCa2-3 labeled with CM-DiI were injected through the tail vein. Four metastatic related gene expressions including MMP-2, MMP-9, VEGF and bFGF were compared between MIA-PaCa2 and MIA-PaCa2-3 cells by Western-blot. 〖WTHZ〗Results〓〖WTBZ〗Pancreatic cancer models with MIA-PaCa2-3 had a significantly higher metastatic rate (8/10) than its parental cells. The expressions of MMP-2, MMP-9 and VEGF were higher in MIA-PaCa2-3 than in MIA-PaCa2. 〖WTHZ〗Conclusions〓〖WTBZ〗MIA-PaCa2-3 cells demonstrate high metastatic potentials in the liver of nude mice. This model is suitable for study of the mechanism and drug testing for pancreatic cancer metastasis.

Targeting expression of TAT－EGFP driven by the prostate specific antigen promoter

JI Kai,WANG Xiao-ling,XU Ai-hui,HU Zhi-min,YUAN Hui-qing

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  742-746. 

Abstract ( 1491 )   PDF (439KB) ( 494 )   Save

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Objective〓〖WTBZ〗We constructed an expression vector containing EGFP fused to the viral TAT transduction domain and driven by the prostate-specific antigen promoter (pPSA-TAT-EGFP) by gene cloning technology. The targeting expression of pPSA-TAT-EGFP was examined in prostate cancer cells and colon cells. 〖WTHZ〗Methods〓〖WTBZ〗The vector pEGFP-1 served as the framework, and the fragment with the TAT sequence and EGFP was produced by using polymerase chain reaction. After digestion with SalI and NotI enzymes, the fragment was inserted into the T cloning vector. The EGFP expression plasmid driven by the PSA promoter (pPSA-EGFP) was digested overnight with SalI and NotI, and a large fragment containing the PSA promoter was purified by gel purification. The TAT-EGFP, cut with SalI and NotI from the T-vector, was linked with the large fragment with the PSA promoter sequence and generated the pPSA-TAT-EGFP construct. The construct was confirmed by restriction enzyme digestion and DNA sequencing, and tested by transfection assay. 〖WTHZ〗Result〓〖WTBZ〗The expression vector pPSA-TAT-EGFP was constructed. It was expressed in prostate carcinoma cells, but was not detected in colon cells. 〖WTHZ〗Conclusion〓〖WTBZ〗A slightly higher expression was observed in cells transfected with EGFP fused to TAT compared to the EGFP control plasmid in prostate cancer cells, but no detectable expression was shown in colon cells.

Establishment of an indirect-ELISA method for detection of HHV-8 infection

QIU Yan,ZHU Hai-feng,ZHANG Xin-sheng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  747-750. 

Abstract ( 1465 )   PDF (353KB) ( 415 )   Save

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Objective〓〖WTBZ〗To establish an indirect-ELISA method for detection of HHV-8 infection and to evaluate its preliminary application significance. 〖WTHZ〗Methods〓〖WTBZ〗The HHV-8 ORF73C gene was amplified with PCR and then was inserted into the prokaryotic-expression vector pGEX-6P-1. The GST-ORF73C fusion protein was expressed and purified from E.coli BL21(DE3) cells. The optimal dilution of coating antigen and serum samples was determined by using the chess titration test. The effect of the ELISA was estimated by an interruption test, a cross-reaction test, a specificity test, and a repetition test, and was compared with that of the K8.1 peptid-ELISA established by Chou et al. 〖WTHZ〗Results〓〖WTBZ〗SDS PAGE and Western blot analysis showed that the target gene
was efficiently expressed in E.coli, and the expressed products showed a fine immunol-reactivity. A chess titration test showed that the optimal concentration of coating antigen was 1.5μg/mL and the optimal dilution of serum samples was 1∶80. An interruption test, cross-reaction test, specificity test and repetition test showed that the ELISA assay had good reproducibility and specificity. 86 serum samples were detected by both GST-ORF73C-ELISA and K8.1 peptide-ELISA, and the coincidence rate of the two methods was 98.8％. 〖WTHZ〗Conclusions〓〖WTBZ〗The GST-ORF73C-ELISA method is specific and repeatable, and is a good method for serological detection and epidemiological analysis for HHV-8 infection.

Dynamic changes of serum NSE and S-100 protein in patients with acute cerebral infarction

LIU Zhen-hua,ZHANG Qing-hua,CUI Yuan-xiao,HAO Mao-lin,ZHENG Mao-yong,PAN Ning,WANG Xiao-hong,ZHANG Gui-ru

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  751-755. 

Abstract ( 1360 )   PDF (295KB) ( 848 )   Save

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Objective〓〖WTBZ〗To investigate the correlation of serum NSE and S-100 protein concentrations with infarct volume and prognosis in acute ischmic stroke. 〖WTHZ〗Methods〓〖WTBZ〗58 patients in Shandong Provincial Hospital with acute ischemic stroke presenting less than 24 hours after symptom onset were incorporated into the study. Serum NSE and S-100 protein levels were determined on hospital admission and 1, 2, 3, 4, 6 and 10 days after symptom onset. Functional outcome in 3 months after stroke (modified Rankin scale score) and final infarct volume on day 7 were determined by means of standardized volumetry of brain images. 〖WTHZ〗Results〓〖WTBZ〗The NSE concentration reached a maximum on day 2. The peak blood level of S-100 proteinwas found on day 3. The level of NSE on day 2 was well correlated with the infarct volume(r=0.823, P＜0.001), and the level of S-100 protein on day 3 was also well correlated with the infarct volume (r=0.937, P＜0.001) and with the clinical outcome assessed with modified Rankin scale score (r=0.507, P＜0.001). 〖WTHZ〗Conclusions〓〖WTBZ〗NSE and S-100 protein in blood during acute stroke are useful markers of infarct volume, and they may serve as easily accessible, inexpensive and valid prognostic markers in the future.

Preliminary analysis of side population phenotypes in the human gastric cancer cell line BGC-823

GENG Li,REN Ning,DONG Wei,YANG Yi,LI Jin-song

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  756-758. 

Abstract ( 1587 )   PDF (321KB) ( 869 )   Save

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Objective〓〖WTBZ〗To explore side population (sp) phenotypes in the gastric cancer cell line BGC-823 and to observe the morph of SP cells and the relationship between SP phenotype and stem cell marker ABCG2. 〖WTHZ〗Methods〓〖WTBZ〗Adherent BGC-823 cells cultured in culture flasks were determined by fluorescence microscopy after being stained with Hoechst33342, and the percentage of SP cells was determined. The adherent cells were mounted on a cover slip and stained with Hoechst 33342, and subsequently stained with mouse anti-human antibody and fluoresence antibody labeled by FITC. The staining of Hoechst33342 and expression of ABCG2 were determined by fluorescence microscopy. 〖WTHZ〗Results〓〖WTBZ〗A SP phenotype was found in the BGC-823 cell line, and it was small and occupied about 1.8% of all tumor cells, and SP cells could express the ABCG2 phenotype. 〖WTHZ〗Conclusion〓〖WTBZ〗SP cells exist in the gastric cancer cell line BGC-823 and can express the stem cell marker ABCG2.

Relationship between chronic glomerulonephritis and the human leukocyte antigen DRB1

WANG Jue,LIU Jun-li,ZHAO Jing-jie

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  759-762. 

Abstract ( 1360 )   PDF (275KB) ( 1004 )   Save

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Objective〓〖WTBZ〗To explore the association between chronic glomerulonephritis(CGN) and the human leukocyte antigen(HLA)-DRB1 on DNA levels and to find susceptibility genes and protective genes, so as to further explain the possible pathogenesis of CGN. 〖WTHZ〗Methods〓〖WTBZ〗1?073 Han renal transplantation patients were included in this study. All patients were recruited from three provincial Hospitals during the past ten years. The control group contained 7418 healthy Han volunteer donors from the Shandong Hematopoietic Stem Cell Data Bank of China. We collected data about the polymorphism of HLA-DRB1. Gene frequency (GF), relative risk (RR)and correlation test were analyzed by using statistical software. Some patients carrying the susceptibility genes were followed up 1, 3 and 5 years, and their survival rates were compared. 〖WTHZ〗Results〓〖WTBZ〗The frequency of the HLA-DRB1*18 allele increased more significantly in the CGN patients than in controls. They might be the suspicious susceptibility genes of CGN. After the follow-up, the prognosis of patients with the susceptibility genes was worse than that of the controls. However, the frequency of the HLA-DRB1*16 alleles decreased more significantly in the CGN patients than that in the controls. They might be the protective genes of CGN. 〖WTHZ〗Conclusion〓〖WTBZ〗There might be corresponding susceptibility and protective genes of CGN in the Shandong Han population, and interestingly, the prognosis of patients with the susceptibility genes is worse than that of the controls.

Muscular ultra-microstructures of the outflow segment of cerebral
bridging veins and its significance on intracranial hypertension

KONG Da-wei,PANG Qi,XU Shang-chen,SI Zhi-chao,WANG Han-bin,ZHU Yu-fang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  763-765. 

Abstract ( 1555 )   PDF (258KB) ( 502 )   Save

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Objective〓〖WTBZ〗To explore the regulatory mechanism of muscular ultra-microstructures of the outflow segments on blood flowing out from the cerebral bridging veins. 〖WTHZ〗Methods〓〖WTBZ〗Bridging veins were obtained from patients suffering from non-venous disorder diseases of the central nervous system. The junctures of bridging veins were completely isolated as close as possible to superior sagittal sinus and sliced. Then serial sections of the outflow segments were observed by transmission electron microscopy. 〖WTHZ〗Results〓〖WTBZ〗Muscular morphological features of
the outflow segments of cerebral bridging veins were obviously different from those of other parts of the cerebral vein system. Sporadic and isolated smooth muscle cells were discovered by electron microscopy, but no nerve fibers and no gap junctions were found between cells. 〖WTHZ〗Conclusion〓〖WTBZ〗The outflow segment of bridging veins can not functionally contract and dilate， the control mechanism here must be passive. Its special morphological pattern indicates that the outflow segment is the possible key point to control vein outflow and so it is a possible key point to regulate intracranial hypertension.

Relationship between Tiam1 expression and peripheral blood micro-metastasis in colorectal carcinoma

ZHUANG Da-yong ,HU San-yuan,FAN Xi-hong,CHEN Ying-jian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  766-769. 

Abstract ( 1562 )   PDF (255KB) ( 431 )   Save

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Objective〓〖WTBZ〗To explore the role of T lymphoma invasion and metastasis factor 1(Tiam1) expression in determining invasion and metastasis, especially the peripheral blood micro-metastasis in colorectal carcinoma. 〖WTHZ〗Methods〓〖WTBZ〗Real-time fluorescence quantitative-polymerase chain reaction (FQ-RT-PCR) was used to determine the expressions of CK20 mRNA and Tiam1mRNA in 51 cases of colorectal carcinoma, and software SPSS 11.5 was used to analyze the relationships between colorectal carcinoma clinical and pathological features and Tiam1 expression. 〖WTHZ〗Results〓〖WTBZ〗CK20 mRNA expression was found in 18 cases(35.29%, 18/51), and Tiam1 mRNA was found in all, however it was （72.11±2.38）×10-4 in cancerous tissues with micro-metastasis and was（15.78±5.01）×10-4 in cancerous tissues without micro-metastasis, and the difference was statistically significant (P＜0.05). Tiam1 expression had no relations to age, gender, primary location, tumor size and peripheral blood carcino-embryonic antigen level , but had relations to tumor differentiation and invasion, lymphocyte metastasis, tumor TNM stage and peripheral blood micro-metastasis （P<0.05）. 〖WTHZ〗Conclusion〓〖WTBZ〗Tiam1mRNA determination is helpful in judging invasion and metastasis of colorectal carcinoma, especially in clarifying peripheral blood micro-metastasis.

Effects of bite force absence on alveolar bone absorption in osteoporosis rats

WANG Xu-xia,ZHANG Jun,LIU Mei,XUE Li-wei,BU Tao

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  770-772. 

Abstract ( 1442 )   PDF (211KB) ( 349 )   Save

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Objective〓〖WTBZ〗To explore the effects of bite force absence on alveolar bone absorption in osteoporosis rats by operation. 〖WTHZ〗Methods〓〖WTBZ〗Forty three-month old Wistar rats were randomly divided into the sham operation group(SO), the loss of stress group(LS)， the ovariectomized group (OVX)，and the ovariectomized with loss of stress group (OVX+LS). Female castration and one side molar absence were prepared by operations. Levels of ALP，Ca and P of blood serum were determined at the 8th and 12th week after the operations, also the bone mineral density (BMD) was determined by the Dual-energy X-ray absorption method. 〖WTHZ〗Results〓〖WTBZ〗ALP of blood serum was increased and the mandible mineral density was degraded in the OVX group, also the mandible mineral density was decreased more significantly in the OVX+LS group after twelve weeks. 〖WTHZ〗Conclusion〓〖WTBZ〗Bite force absence aggravates alveolar bone absorption in osteoporosis rats.

Effect of calcium in ropivacaine-induced smooth muscle contraction of the aorta in rats in vitro

WANG Shu-qin,QI Feng,WU Jian-bo,SUN Bao-zhu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  773-776. 

Abstract ( 1420 )   PDF (356KB) ( 336 )   Save

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Objective〓〖WTBZ〗To explore the role of calcium in ropivacaine-induced contraction of vascular smooth muscle. 〖WTHZ〗Methods〓〖WTBZ〗Endothelium-denuded rat aortic rings and segments were prepared. The cumulative dose-contractile response relationships of aortic rings to ropivacaine were tested in the presence or absence of different concentrations of the L-type voltage-dependent calcium channel blocker, nicardipine, the inositol trisphosphate receptor blocker, 2-APB, or the lower extracellular calcium concentrations by using isometric force transducers. The cumulative dose-related increases in intracellular calcium concentration (［Ca2+］i) of aortic segments to ropivacaine were determined in the presence or absence of nicardipine, 2-APB, or the extracellular calcium-free by using a fluorimeter. 〖WTHZ〗Results〓〖WTBZ〗Ropivacaine induced a dose-dependent contractile response of rat aortic rings: increasing at concentrations of 0.03mmol/L to 0.3mmol/L and decreasing from 1 mmol/L to 3mmol/L, which was attenuated in a dose-dependent manner by nicardipine (1nmol/L, 5nmol/L and 10nmol/L), 2-APB (5μmol/L, 10μmol/L and 50μmol/L) and lower extra-cellular calcium concentrations (2mmol/L, 10mmol/L and 0). Ropivacaine also induced an increase in ［Ca2+］i in accordance with the contractile responses, which was also significantly inhibited by nicardipine (10?nmol/L), 2-APB (50μmol/L), and extra-cellular calcium-free. 〖WTHZ〗Conclusion〓〖WTBZ〗The increase in ［Ca2+］i by the extra-cellular calcium influx and the calcium release from the sarcoplasmic reticulum is involved in ropivacaine-induced vasoconstriction of rat aortic smooth muscle.

Thoracic epidural anesthesia combined with general anesthesia can reduce the intestine barrier dysfunction

WANG Huan-liang,ZHANG Li,LIU Rui-fang,SUN Bao-zhu

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  777-780. 

Abstract ( 1304 )   PDF (293KB) ( 391 )   Save

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Objective〓〖WTBZ〗 To investigate the effect of thoracic epidural anesthesia combined with general anesthesia on the intestine barrier function. 〖WTHZ〗Methods〓〖WTBZ〗Twenty ASA class I-II patients undergoing elective upper abdominal surgeries were randomly divided into the general anesthesia group (group GA, n=10) and the general anesthesia combined with thoracic epidural anesthesia (TEA)group (group GE, n=10). Both groups received phenobarbital sodium 0.1g and atropine 0.5mg im 30 min prior to the surgery. In the GE group an epidural catheter was inserted through a needle placed at T 8-9 or T9-10 and cephalad for 3.5cm, and then 10ml 0.5% bupivacarine was injected into the epidural spare after giving a tail dose of 3 ml 2% lidocarine. In both groups anesthesia was induced with 0.05mg/kg midazolam, 3μg/kg fentanyl, 0.1mg/kg vecuronium and 0.15-0.25mg/kg etomidate and maintained with inhalation of 1%-2.5% isoflurance and inter-mitten iv boluses of fentanyl and vecuronium. After the operations, tramadol intravenous pump was given to the GA group and bupivacarine epidural pump was given to the GE group. The plasma D-lactate and Lip polysaccharide (LPS) levels and the intestine microbial DNA were determined the day before the operation (T1) and 0 (T2), 24 (T2) and 48 h(T4) after the operation by PCR.〖WTHZ〗 Results〓〖WTBZ〗Plasma D-lactate and LPS were significantly increased at T2, T3, and T4 compared with T1 and were higher in group GA than in group GE (P<0.05). Bacterial DNA was found in 4 patients in group GA and 2 patients in group GE (χ2=42.27, P<0.01). Also SIRS was found in 6 patients in group GA and 4 patients in group GE during 24h after surgeries (χ2=37.64, P<0.01).〖WTHZ〗 Conclusion〓〖WTBZ〗TEA combined with general anesthesia can reduce the intestine barrier dysfunction after upper abdominal surgery.

Regular follow-up and pacemaker parameter program on psychological conditions of patients with a cardiac pacemaker

XU Dong-ling,MA Cheng-en,WANG Su-jia

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  781-783. 

Abstract ( 1395 )   PDF (223KB) ( 602 )   Save

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Objective〓〖WTBZ〗To explore the psychological conditions of patients with a cardiac pacemaker after a regular follow-up and pacemaker parameter programming. 〖WTHZ〗Method〓〖WTBZ〗28 patients were randomly selected as the normal follow-up group and were followed up at 3 months, 1, 3, and 6 years after implantation and pacemaker program performance was determined when needed by patients. Another 30 patients served as the follow-up treatment group and were followed up and programmed at 3 months and every 6 months after implantation. Cardiac symptoms questionnaires and symptom checklists (SCL-90) were filled out by all patients at 3 months, 3 and 6 years after implantation. 〖WTHZ〗Results〓〖WTBZ〗There were no differences of cardiac symptoms between the follow-up treatment group and the normal follow-up group. But significant differences were found in SCL-90 scores between the two groups, the former was better than the latter. Degree of depression, anxiety and other symptoms were lower in the follow-up treatment group than in the normal follow-up group (P＜0.05). Depression, anxiety and other symptoms of the paced patients were dynamically changed time dependently after implantation. 〖WTHZ〗Conclusion〓〖WTBZ〗Regular follow-up and pacemaker parameter program have positive influence on psychological conditions of patients with a cardiac pacemaker by improving depression, anxiety and other symptoms.

CT perfusion imaging and determination of Glu and GABA content in acute cerebral infarction

MAN Xiao,LENG Zhen-pu,PANG Zai-ying,DU Yi-feng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  784-786. 

Abstract ( 1290 )   PDF (298KB) ( 317 )   Save

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Objective〓〖WTBZ〗To investigate the application of computed tomography perfusion imaging（CTPI）combined with determination of Glu and GABA in the plasma in diagnosis of acute cerebral infarction (CI). 〖WTHZ〗Methods〓〖WTBZ〗57 patients with acute cerebral infarction onset within 24 hours were examined by conventional CT and CTPI. CTPI yielded a set of relative cerebral blood flow(rCBF), mean transmit time(MTT) and time to peak(TP). By mean of highperformance liquid chromatography, the levels of Glu and GABA in the plasma of all patients were determined. 〖WTHZ〗Results〓〖WTBZ〗Conventional CT scan showed a low-density signal in 26 of 57 patients. CTPI revealed normal perfusion in 3 patients and hypo-perfusion in 54 patients. The regional cerebral blood flow（rCBF）, time to peak（TP）, and mean transit time（MTT）were obviously changed in 54 patients by CTPI. The ischemic lesion side as compared with the opposite side, and the core of the ischemic lesion as compared with the peripheral zone were significantly changed（P＜0.05）. A linear correlation analysis showed that there were positive correlations between infarction volume and plasma levels of Glu and GABA (r=0.88, P＜0.01; r=0.47, P＜0.01) and the infarction volume. 〖WTHZ〗Conclusion〓〖WTBZ〗CTPI combined with determination of Glu and GABA in plasma can be used in diagnosis of early stage of cerebral infarction and measurement of infarct volume, so it is great for prognosis and treatment.

Ringer′s solution and Synvisc in treatment of knee osteoarthritis: a contrast study

TENG Xue-ren,HU Guang-liang,ZHAO Yong-sheng,ZHOU Lun,LI Jian-min

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  787-790. 

Abstract ( 1809 )   PDF (304KB) ( 519 )   Save

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Objective〓〖WTBZ〗To explore the effects of Ringer′s solution and Synvisc in treatment of knee osteoarthritis(OA). 〖WTHZ〗Methods〓〖WTBZ〗A total 58 outpatients with knee osteoarthritis were randomly divided into three groups. Group A accepted simple intra-articular irrigation of Ringer′s solution, group B accepted simple Synvisc injection and group C accepted both intra-articular irrigation of Ringer′s solution and Synvisc injection. Westem Ontario and McMaster University Osteoarthritis Index (WOMAC) pain score were used to assess the effects before treatment and[JP2]1, 8, 12, 24 and 52 weeks after treatment. All treatments were finished by the sam doctor group in the Qingdao Municipal Hospital by a patient-blinded method. All analyses were performed by the Stata software. 〖WTHZ〗Results〓〖WTBZ〗All three groups had the WOMAC pain scores significantly[JP2] improved at 8, 12 and 24 weeks(P＜0.05), and groups B and C still had significant effects at 52 weeks (P＜0.05). Groups B and C were superior to group A in clinical efficacy(P＜0.05), and group C was superior to group B in pain relief at 1-week term(P＜0.05). 〖JP2〗〖WTHZ〗Conclusions〓〖WTBZ〗Either Ringer′s solution or Synvisc injection can significantly improve the WOMAC pain scores for knee osteoarthritis, and Synvisc injection is superior to Ringer′s solution in pain-relief, duration and operative briefness， but there are no folded effects when they are used in combination.

Ximingting tablets in the treatment of women with climacteric syndrome

LIU Pei-shu,LI Xia,LIU Yuan,MAO Hon-luan,SUN Yu-lan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  791-794. 

Abstract ( 2440 )   PDF (284KB) ( 638 )   Save

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Objective〓〖WTBZ〗To observe the efficacy and safety of Ximingting tablets in the treatment of women with climacteric syndrome. 〖WTHZ〗Methods〓〖WTBZ〗This was a randomized, double-blind and controlled trial. Sixty patients with climacteric symptoms wererandomly assigned into two parallel groups: the investigated group and the Beimeili-controlled group. The climacteric symptoms, such as flushes, sweating, and irritation were studied before and after treatment. Changes of blood routine, urine routine, liver and kidney function and hormones(E2, FSH) were also assessed. 〖WTHZ〗Result〓〖WTBZ〗After treatment, hot flushes, sweating and vertigo in the two groups showed no significant differences（P＞0.05）, however, irritation and insomnia, depression, weakness, heart-throb in the investigated group were superiorto those in the Beimeili-controlled group（P＜0.05）, while arthralgia, headache and formication in the investigated group were inferior to those in the Beimeili-controlled group（P＜0.05）.　Blood routine, urine routine, liver and kidney function and hormones(E2, FSH) in the two groups had no significant differences（P＞0.05）. 〖WTHZ〗Conclusion〓〖WTBZ〗Ximingting tablets are efficacious and safe for the treatment of women with climacteric syndrome.

Micro-calcification in ultrasound diagnosis of breast diseases

WANG Hai-ying,ZHANG Hua-wei,ZHANG Qi-bo,XIN Hua,YU Guo-fang

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  795-797. 

Abstract ( 1456 )   PDF (252KB) ( 476 )   Save

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Objective〓〖WTBZ〗To evaluate the value of high-frequency ultra-sonography(US) in the diagnosis of breast diseases with micro-calcification. 〖WTHZ〗Methods〓〖WTBZ〗Sixty-eight patients with breast micro-calcification received mammography and high-frequency US. Based on size, micro-calcifications were divided into two groups: the≥10mm group and the ＜10mm group, also based on distribution, they were divided into the cluster, linear and scattered distribution groups. Sensitivity and specificity of US in the diagnosis of breast diseases were analyzed. 〖WTHZ〗Results〓〖WTBZ〗Sensitivity and specificity of US in breast malignant diseases with micro-calcification was 92.1% and 76.7%. 61.7% of the breast micro-calcifications were found by US, and 84.2% of them were malignant and 33.3% were benign (P＜0.05). The detection rate of a micro-calcification cluster larger than 10mm was 84.8% and of that smaller than 10mm was 40.0%; and the detection rate of the micro-calcification of linear distribution and cluster distribution was 72.5% and of that scattering distribution was 29.4%. 〖WTHZ〗Conclusion〓〖WTBZ〗US can depict breast micro-calcifications, especially malignant lesions, and micro-calcification has a great value for the diagnosis of breast diseases.

Contrast quantity and injection velocity in coronary angiography with 64 multi-detector CT

ZHAO Lei,ZHAO Qiao-jing,WANG Xi-lin,ZHANG Wei,SHAO Guang-rui

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  798-800. 

Abstract ( 1297 )   PDF (269KB) ( 720 )   Save

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Objective〓〖WTBZ〗To explore the effect of contrast quantity and injection velocity in 64 multi-detector CT. 〖WTHZ〗Methods〓〖WTBZ〗Ninety patients diagnosed as or suspected coronary heart diseases (CAD) were subjected to CT coronary angiography using 64 multi-detector CT. Based on contrast quantity and injection velocity, they were divided into three groups: group A(65mL, 5.0mL/s), group B (45mL,5.0mL/s) and group C(45mL,4.5mL/s).The imaging was analyzed by the coronary modified fraction method, American Heart Association. 〖WTHZ〗Results〓〖WTBZ〗There were no statistical differences between group A and B and between group A and C. 〖WTHZ〗Conclusion〓〖WTBZ〗Contrast quantity and injection velocity may be decreased, but the imaging should still be clear.

Acellular dermal matrix in the prevention of Frey syndrome after parotidectomy

DONG Zuo-qing,ZHANG Fan,WEI Feng-cai,PENG Hua-hai,TAN Wan-ye

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  801-803. 

Abstract ( 1955 )   PDF (228KB) ( 561 )   Save

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Objective〓〖WTBZ〗To evaluate the effect of acellular dermal matrix in preventing development of Frey syndrome after parotidectomy. 〖WTHZ〗Methods〓〖WTBZ〗156 patients were divided into 2 groups: the experimental group subjected to superficial parotidectomy with placement of an acellular dermal matrix and the control group subjected to superficial parotidectomy without placement of an acellular dermal matrix. All were evaluated 12 to 37 months later and questioned about gustatory sweating. The Minor starch-iodine test was performed on for an objective assessment. 〖WTHZ〗Results〓〖WTBZ〗Subjective Frey syndrome was found in 4 patients in the experimental group and 32 in the control group. And objective Frey syndrome was found in 7 patients in the experimental group and 46 in the control group. Both subjective and objective differences in Frey syndrome were statistically significant. 〖WTHZ〗Conclusion〓〖WTBZ〗An acellular dermal matrix can reduce the incidence of Frey syndrome, and it is safe and has no side effects.

Changes of the AST and ALP levels in gingival crevicular fluid during orthodontics treatment

REN Xu-sheng,WANG Xu-xia,ZHANG Wen-juan,XU Jian-guang,ZHANG Jun

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  804-806. 

Abstract ( 1396 )   PDF (215KB) ( 489 )   Save

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Objective〓〖WTBZ〗To explore the effect of AST and ALP changes in gingival crevicular fluid for measurement of periodontal tissue health status during orthodontics therapy. 〖WTHZ〗Methods〓〖WTBZ〗44 teenager patients using a fixed appliance were enrolled in this study. Levels of AST and ALP in gingival crevicular fluid were determined by using biochemistry analysator before treatment， and one month and three months after treatment. 〖WTHZ〗Results〓〖WTBZ〗Levels of AST and ALP significantly increased one month after treatment but became normal three months after treatment. Gingival indexes of 1 month also significantly increased (P＜0.05), but they were not statistically different between the pre-treatment and the 3-month post-treatment period(P＞0.05). 〖WTHZ〗Conclusion〓〖WTBZ〗AST and ALP can be used to measure periodontal tissues.

Control of dental plaque on helicobacter pylori in gastric mucosa

JIA Chun-ling,YANG Xue-xin,WANG Lei,LI Cui-rong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  807-808. 

Abstract ( 1227 )   PDF (149KB) ( 595 )   Save

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Objective〓〖WTBZ〗To explore the relationship between oral and gastric mucosa helicobacter pylori (H. pylori). 〖WTHZ〗Methods〓〖WTBZ〗108 patients without H. pylori in their gastric mucosa were assigned into the experimental group (n=56) and the control group (n=52). Dental plaque was completely controlled in the experimental group and was not controlled or not successfully controlled in the control group. All patients re-underwent endoscopies after half a year. By endoscopies, biopsies were taken from the gastric mucosa. Histological examination and rapid Urease test were used to determine the presence of H. pylori. 〖WTHZ〗Results〓〖WTBZ〗H. pylori was found in 11 cases (19.6%) in the experimental group and 42 cases (80.8%) in the control group. The presence of H. pylori was significantly lower in the experimental group than that in the control group (P＜0.05). 〖WTHZ〗Conclusion〓〖WTBZ〗There are significant relationships between oral and gastric H. pylori infection, indicating dental plaque should be controlled in treatment of H. pylori-related gastric diseases.

Characteristics and risk factors of cerebral infarction in type 2 diabetics

LIANG Wen-long,ZHAO Xiao-dong,WANG Shu-juan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  809-811. 

Abstract ( 1397 )   PDF (287KB) ( 723 )   Save

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Objective〓〖WTBZ〗To analyze the clinical characteristics and risk factors of type 2 diabetics with cerebral infarction in senile patients. 〖WTHZ〗Method〓〖WTBZ〗56 patients with type 2 diabetics and cerebral infarction (group A), 50 with type 2 diabetics but without cerebral infarction (group B) and 79 without diabetes mellitus (group C) were enrolled in this study. Waist circle(Wc), body mass index（BMI）, blood pressure, blood fat, blood glucose (BG), glycosylated hemoglobin (HbA1C), fasting insulin, leptin and adiponectin were determined in each group. 〖WTHZ〗Results〓〖WTBZ〗Recurrent, multi-focal and large-area infarcts were more common in group A than in group C, and levels of Wc, BMI, systolic blood pressure (SBP) and triglyceride(TG) wereincreased, and density lipoprotein cholesterol(LDL-C), apolipoprotein B100(ApoB100), BG, HbA1C, fasting insulin and leptin were decreased in group A compared with group B（P＜0.05), while levels of adiponectin were decreased and density lipoprotein(HDL)and apolipoprotein A1(ApoA1) were increased in group A （P＜0.05）. Logistic regression analysis showed that BMI, SBP, TG, LDC-C, postprandial twohour blood glucose(PBG), leptin and adiponectin statistically related to cerebral infarction. [WTHZ]Conclusion〓〖WTBZ〗Recurrent, multi-focal and large-area infarcts are more common in group A. High BMI, SBP,TG, PBG, insulin level and leptin and low adiponectin are potential risk factors of cerebral infarction in diabetics.

Injury-related factors in college students: a paired case-control study

ZHANG Yuan ,LI Hua-feng,HAN Xia,ZHANG Wei,ZHAO Zhong-tang,JIA Cun-xian

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  812-814. 

Abstract ( 1140 )   PDF (296KB) ( 398 )   Save

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Objective〓〖WTBZ〗To explore the risk and protective factors of injuries among college students.〖WTHZ〗 Methods〓〖WTBZ〗A total of 1929 students were investigated by cluster sampling, 280 students with injuries in 2006 served as the experimental group and 280 1∶1 matched students without injuries served as the control group. The 280 students without injury as controls were randomly selected by the same gender, age, department and nation as students with injuries. Data were analyzed by conditional univariate and multivariate logistic regression analyses. 〖WTHZ〗Results〓〖WTBZ〗The incidence ratio of injury in college students was 14.52％, and that of fall／collision injury was the highest (40.71％). non-only child, father with low education and high nervous latitude score were risk factors and the OR values were respectively 1.718(95％CI:1.252～2.357), 1.282 (95％CI:1.055～1.568) and 1.121(95％CI:1.087～1.156), and maternal occupation and low latitude stability score were protective factors and the OR values were 0.732 (95％CI:0.619～0.865) and 0.975(95％CI:0.954～0.997). 〖WTHZ〗Conclusion〓〖WTBZ〗College policy-makers should develop effective measurements to reduce health-risk behaviors with family and society and promote students′ self-protective consciousness.

Relationship between time and concentration of Lacto-tinidazole after drug combination

FANG Ying-li,LIU Ai-ting,ZHOU Wen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  815-818. 

Abstract ( 1319 )   PDF (262KB) ( 668 )   Save

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Objective〓〖WTBZ〗To offer a basis for rational clinical use of tinidazole and to guide breast feeding to neonates. 〖WTHZ〗Method〓〖WTBZ〗The Lacto-tinidazole concentrations at different times after intravenous drip tinidazole (800mg/d) were determined by HPLC using the self-control method. 〖WTHZ〗Results〓〖WTBZ〗Lacto-tinidazole concentration was statistically different among different patients and 0, 2, 4, 6, 8, 18 and 24h（P＜0.05）after the same patient received intravenous drip of tinidazole after two or three days. 〖WTHZ〗Conclusion〓〖WTBZ〗Tinidazole can be accumulated in patients after three days usage. So it should be cautiously used so as to reduce neonates passive absorption.

Interaction of triazole agents and Cyclosporin A against Candida albicans isolates in vitro

YU Jin-long ,LI Yan,GUO Qiong-jie,SUN Shu-juan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  819-822. 

Abstract ( 1455 )   PDF (294KB) ( 463 )   Save

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Objective〓〖WTBZ〗To explore the interaction of three azoles combined with Cyclosporin A against susceptive and resistant strains of C. albicans. 〖WTHZ〗Methods〓〖WTBZ〗The response was determined by the checkerboard micro-dilution method， and after the spectrophototric reading， the fractional inhibitory concentration index（FICI）was calculated. 〖WTHZ〗Results〓〖WTBZ〗Drugs combined together acted synergistically against resistant strains， while the interactions were mostly indifferent against susceptible strains. 〖WTHZ〗Conclusion〓〖WTBZ〗Cyclosporin A largely enhances the anti-fungal activity of azoles against resistant C.albicans strains but not against susceptive ones. The OD testing method is objective and convenient.

Determination of curcumol in animal plasma and tissues by HPLC-MS/MS

HAO Zhen-feng,WANG Ben-jie,ZHANG Rui,WEI Chun-min,YUAN Gui-yan,GUO Rui-chen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(8):  823-827. 

Abstract ( 1336 )   PDF (267KB) ( 358 )   Save

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Objective〓〖WTBZ〗To develop high performance liquid chromatography coupled with the tandem mass spectrometry(HPLC-MS/MS) quantitative detection method for determination of curcumol in animal plasma and tissues. 〖WTHZ〗Methods〓〖WTBZ〗Dog plasma and rat tissue samples spiked with ornidazole as the internal standard were separated on a Diamonsil C18 column and eluted at a flow rate of 1mL/min, and the precursor/ product ion combinations of m/z 254.3→219.4 and m/z 220.3→128.1 were monitored to quantify curcumol by MRM. 〖WTHZ〗Results〓〖WTBZ〗A good linearity of plasma or tissue curcumol concentration and area ratio of curcumol to i.s was obtained. 〖WTHZ〗Conclusion〓〖WTBZ〗The developed method is rapid, highly sensitive and specific and could be used in animal curcumol pharmacokinetic and tissue distribution studies.