Abstract:

Objective〓〖WTBZ〗To establish a pancreatic cancer nude mice model with high metastatic potential for study of the mechanism and drug screening of pancreatic cancer liver metastasis. 〖WTHZ〗Methods〓〖WTBZ〗Human pancreatic cancer cell lines MIA-PaCa2 were injected into the spleen, and cancer cells from liver metastatic foli of mice were cultured in vitro and subsequently injected into the spleen. After the procedures were performed three times, a sub-line of MIA-PaCa2 with high livermetastatic potential MIA-PaCa2-3 labeled with CM-DiI were injected through the tail vein. Four metastatic related gene expressions including MMP-2, MMP-9, VEGF and bFGF were compared between MIA-PaCa2 and MIA-PaCa2-3 cells by Western-blot. 〖WTHZ〗Results〓〖WTBZ〗Pancreatic cancer models with MIA-PaCa2-3 had a significantly higher metastatic rate (8/10) than its parental cells. The expressions of MMP-2, MMP-9 and VEGF were higher in MIA-PaCa2-3 than in MIA-PaCa2. 〖WTHZ〗Conclusions〓〖WTBZ〗MIA-PaCa2-3 cells demonstrate high metastatic potentials in the liver of nude mice. This model is suitable for study of the mechanism and drug testing for pancreatic cancer metastasis.

Key words: Pancreatic Neoplasms, Neoplasm Metastasis, Liver, Mice, nude