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Table of Content

      
    16 September 2008
    Volume 46 Issue 9
    Articles
    Articles
    Expression of Tribbles in human monocyte-driven macrophages and the regulative role of ox-LDL on them
    SHANG Yuan-yuan,ZHANG Wei,ZHANG Li-ping,DENG Jing-ti,TANG Meng-xiong,ZHONG Ming,ZHANG Yun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  833-836. 
    Abstract ( 948 )   PDF (373KB) ( 276 )   Save
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    To investigate the expression of Tribbles (TRBs) in human monocytedriven macrophages and to analyze the regulative role of oxidized low density lipoprotein (ox-LDL) on TRB1, TRB2 and TRB3. MethodsPrimary human monocyte-driven macrophages were cultured in vitro. Macrophages were incubated with ox-LDL and low density lipoprotein (LDL) at various concentrations for various times. The level of TRBs mRNA was determined by quantification real-time RT-PCR. ResultsAll three TRBs genes (TRB1, TRB2 and TRB3) were expressed in human monocyte-driven macrophages, among them, TRB3 was predominant, and TRB1 and TRB2 were little expressed. OxLDL increased the levels of TRB3 andTRB2 mRNA, while it decreased the expression of TRB1 in a dose-and time-dependent manner. Conclusions All three TRBs are expressed in human monocytedriven macrophages and can be regulated by ox-LDL, so TRBs, especially TRB3 may participate in the course of macrophage apoptosis.

    Effects of dehydroepiandrosterone on experimental autoimmune
    neuritis in Lewis rats
    TAN Xiao-dong,DUAN Rui-sheng,SHI Chang-wen,SUN Ruo-peng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  837-841. 
    Abstract ( 1059 )   PDF (689KB) ( 465 )   Save
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    To explore the effects of dehydroepiandrosterone (DHEA) on the development of experimental autoimmune neuritis (EAN) and to explore its mechanism. Methods Thirty-six Lewis rats were randomly divided into the DHEA 0.5mg treatment group, the DHEA 2mg treatment group and the control group (n=12). The treatment groups were subcutaneously injected every day with DHEA and the control group was subcutaneously injected with the same volume of DHEA dissolvent from day 5 post-immunization (p.i.) to day 28 p.i. with bovine peripheral myelin（BPM）in Freund′s complete adjuvant (CFA). The effects were assessed in terms of appearance of clinical signs, clinical score, histopathology and IFN-γ and TNF-α positive cells in sciatic nerve sections, and T-cell proliferation and inflammatory cytokines (IFN-γ,TNF-α and IL-10) synthesis by draining lymph nodes and spleen cells. ResultsRats receiving DHEA in the two different therapeutic regimens displayed a significant delay in onset (P<0.05 and P<0.05), a decreased inflammatory cell infiltration into the PNS (P<0.05,P<0.05) and decreased numbers of IFN-γ and TNF-α expression cells in the PNS(P<0.05 and P<0.05 ), and reduced BPMstimulated T cell proliferation(P<0.05 and P<0.05) and IFN-γ and TNF-αsecretion in the draining lymph node and spleen (P<0.05, P<0.05) compared to the control group. Only the DHEA 2mg treatment group had the significant differences of supernatant IL-10 were found among the different treatment and control groups（P>0.05）.ConclusionAdministration of exogenous DHEA ameliorates the severity of EAN, presumably by suppressing the proliferation of auto-reactive T-cells and moderating the over-expression of pro-inflammatory cytokines. DHEA 2mg therapy is more effective than DHEA 0.5mg therapy.
    Quantitative analysis of calcium content in aortic valved homograft tissues in rats
    GONG Xing-jun,ZHANG Gong,PANG Xin-yan,LI Shou-xian,GU Xing-hua
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  842-846. 
    Abstract ( 957 )   PDF (483KB) ( 213 )   Save
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    To investigate factors affecting calcium content in aortic valved homograft(AVH) tissues in rats. Methods90 Wistar rats were randomly allocated into 3 groups: two allogene groups and one isogenenic group. Allogene group: SD→Wistar, one group received AVH of cryopreservation, the other group received AVH preconditioned with dendritic cell monoclonal antibodies（DCmAb ）diluted by 1:5 and cryopreserved, and then was treated with DcmAb. Isogenenic group: Wistar→Wistar. AVH with myocardial cuff was heterotopically allografted onto the abdominal aorta of rats. Rats were postoperatively sacrificed in batches at 2, 4, 8, 12 and 16 weeks. Blood samples were obtained for detection of the TCRα, β and CD28 expressions, and the AVH specimens were obtained for detection of the changes of endotheliocyte and smooth muscle cells with a light and electron microscope, and the CD54 expression by immunohistochemistry. Also, the calcium contents of the AVH tissues after transplantation were determined. Results① Compared with the isogenic group, the expression of TCR-α, β and CD28 in the allogene group was significantly increased at each time point(P<0.01). Compared with the cryopreservation group, the expression of TCR-α, β and CD28 in the DCmAb treatment group was significantly decreased(P<0.01). ② The calcium contents of AVH tissues in the allogene group gradually increased at 4 weeks, which was significantly different from that in the isogenenic group over 5 different periods (P>0.05). In the cryopreservation group, the calcium contents of the AVH tissues were 2856±79 and 3587±168 at 4 and 8 weeks post-operatively; were 2176±210, 3089±176 in the DCmAb treatment group; and were 860±60, 870±50 in the isogenic group. Conclusion Immunosuppressive treatment has obviously effect in lightening the immune rejection response and delaying the AVH calcification course.
    Expression of peroxisome proliferator-activated receptor γ in myocardial cells and microvasculature of diabetes myocardial fibrosis in rats
    XU Dong-ling,LIU Wei-hua,DU Yi-meng,JIANG Wei-dong,QIN Ai-qiong,LU Qing-hua,LU Shu-xia,GE Zhi-ming,SUI Shu-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  847-850. 
    Abstract ( 1148 )   PDF (733KB) ( 390 )   Save
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    To investigate the role of peroxisome proliferator-activated receptor γ (PPARγ) in the process of diabetes myocardial fibrosis in rats. MethodTwentyseven male Wistar rats were randomly divided into two groups: the DM group (n=15), which were induced by a single intraperitoneal injection of STZ (65mg/kg body wt) and the control group (n=12). All rats were fed normally for 5 months and eleven DM rats survived (n=11). The radiate escape pestilence method was used to determine the level of plasma angiotensin II (Ang II). HE staining and Masson staining were used to test collagen. Also, immunohistochemistry was used to determine the protein expressions of PPARγ and Ang II. ResultsThe plasma AngⅡ level was higher in the DM group. Cardiac collagen deposition was obviously enhanced in the DM groups compared with the control groups. Expression of PPARγ in the DM group was higher than that in the control group, especially in the capillary endothelium and the myocardial cells. The expression of AngⅡ in the cytoplasm of myocardial cells in the DM group was higher than that in the control group. Conclusion The DM animal model was successfully established. Expression of PPARγ in myocardial cells and capillary endothelium significantly increases in diabetes rats. PPARγ may play an important role in the process of diabetes myocardial fibrosis.
    Effects of pioglitazone on the ghrelin expression in type 2 diabetic rats
    DONG Ming,HOU Xin-guo,CHEN Li,SUN Lei,FU Yi-ling,Sun Yu,WANG Hui
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  851-854. 
    Abstract ( 1102 )   PDF (337KB) ( 462 )   Save
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    To explore the effects of pioglitazone on the plasma ghrelin levels and the ghrelin mRNA expression in the stomach of type 2 diabetic rats. MethodsWistar rats were fed with a high fat diet for 8 weeks and then treated with a low dose of STZ(30mg/kg) by intraperitoneal injection to establish type 2 diabetic rat models, then they were divided into three groups: the control group(NC, n=10), the diabetes group(DM, n=12) and the pioglitazone treatment group(DM-PIO, n=12). Rats in the DM-PIO group were treated with pioglitazone(20mg·kg-1·d-1) by intra-gastric administration. All the animals were sacrificed 8 weeks later. The blood glucose, lipids, insulin, leptin and ghrelin were determined, and the ghrelin mRNA expression in the stomach was determined by RT-PCR. ResultsThe DM and DM-PIO groups had significant reduced plasma levels of ghrelin and mRNA expression levels in the stomach compared with the NC rats (P＜0.05 for each group). Plasma ghrelin and mRNA expression levels were higher in the DM-PIO rats than in the DM rats, which was not significant (P＞0.05). By the correlation analysis, plasma ghrelin levels were negatively correlated with body weight (P＜0.05), HOMAIR (P＜0.01) and leptin P＜0.05) in diabetic rats. ConclusionThe plasma ghrelin levels and the expression of ghrelin in diabetic rats are significantly reduced. Pioglitazone can not change the plasma levels of ghrelin and its expression in the stomach.
    Transcranial magnetic stimulation motor evoked potentials and pathologicchanges in rabbit spinal cord injury
    HOU Yong,NIE Lin,WU Qi,SHAO Jun,LV Li-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  855-858. 
    Abstract ( 1068 )   PDF (640KB) ( 418 )   Save
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    To explore the changes of transcranial magnetic stimulation motor evoked potentials(TMSMEP) and spinal cord pathology in rabbit spinal cord injury, and to explore their correlation. MethodsA digital weight drop apparatus induced spinal cord lesion was performed to mimic human SCI. Based on the drop height, 32 rabbits were randomly divided into four groups: the control, 2cm, 4cm and 6cm injured groups. TMSMEP was used to assess neurologic function. The MEP was recorded at 5min, 15min, 30min, 60min, 6h, 24h, 3d, 7d post-injury and before spinal cord injury in injury groups. Tarlov behaviour score was performed before and after surgery at 1, 3, 7 days. Light and electron microscope were used to observe the pathological changes. Results Spinal cord injury resulted in a gradual decrement in the peak latency and significant increment in the peak amplitude. Simultaneously, histological studies showed that the drop causes a ischemia lesion, which was found in human spinal cord injury. ConclusionTMSMEP has a significant association with spinal cord pathological changes and will be a noninvasive examination for spinal cord function.
    Protective effects of N-acetylcysteine on oxygen-glucose deprivation injured cortical astrocytes in rats
    QIN Wen,ZHANG Xiao-xia,LIU Ping,WANG Zi-ying
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  859-862. 
    Abstract ( 1032 )   PDF (282KB) ( 345 )   Save
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    To investigate whether N-acetylcysteine has protective effects on oxygen-glucose deprivation injured cortical astrocytes in rats. Methods The fourth passage of the cortical astrocytes of primary cultured neonate rats was used. Na2S2O4 and sugar free Earle′s solution were used to establish the chemical oxygen glucose deprivation (OGD) model, which followed by changing the culture into complete mediums to mimic ischemia/reperfusion injury. The 3-(4,5-dimethylthialzol-2-yl)-2-5 -diphenyl-tetrazolium bromide (MTT) method and the lactate dehydrogenase (LDH) leakage method were used for evaluations of cell viability and cell lysis, and the Colorimetric method was used to determine changes of cerebral energy metabolism. Results Compared with the sham-group, the cultured neonate rat cortical astrocytes were subjected to one hour of OGD followed by 24 hours of reperfusion, then the cell viability was decreased and the cell lysis was significantly increased, and the cellular energy metabolism was disordered. Pre-treatment with N-acetylcysteine can dose-dependently reverse these injuries. Conclusion N-acetylcysteine exhibits protective effects on OGD/reoxygenation injuries in rat cortical astrocytes.
    Effects of ozone on rat astrocytes in vitro
    ZHOU Nai-bao,FU Zhi-jian,SUN Tao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  863-865. 
    Abstract ( 944 )   PDF (279KB) ( 290 )   Save
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    To explore the effects of ozone on astrocytes in vitro and to investigate its mechanism. MethodsAstrocytes were cultured in vitro in accordance with McCarthy′s method and were identified by immunohistochemistry staining. Then cells were seeded into 24well plates and divided into 3 groups(n=7): the oxygen group(O2 group), the ozone 40(O340) group and the ozone 80(O380) group to which was respectively added 400μL complete medium(CM) acted by oxygen for 20min, 400μL CM acted by 40μg/mL ozone for 20min and 400μL CM acted by 80μg/mL ozone for 20min. After incubation for 2h or 4h, the lactate dehydrogenase (LDH) leaking ratio was determined by the simple method of Qingtao Hong and dead cell percentage was determined by trypan blue staining. ResultsThe LDH leaking ratio was decreased (P＜0.05) in the O340 group and was increased (P＜0.01) in the O380 group compared with the O2 group, and also it was increased (P＜0.05) in the 4haction O380 group compared with the 2haction O3 80 group. Dead cell percentage in the O380 group was increased (P＜0.01) compared with the O2 group and also it was increased (P＜0.05) in the 4haction group compared with the 2haction group. Conclusion 80mg/L ozone acting in the short term (2 or 4 hours) has a damage role on astrocytes, while 40mg/L ozone does not.
    Correlation between state-trait anxiety, personality, coping style and the cortisol level in medical freshmen
    JIANG Hong,ZHANG Qian,SUN Yu,PAN Fang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  866-868. 
    Abstract ( 1029 )   PDF (260KB) ( 494 )   Save
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    To explore the correlation between state-trait(SA) anxiety and personality(P), coping style and to explore whether trait anxiety influences the salivary cortisol level. MethodThe SA anxiety, personality and coping style were measured by using questionnaires, and the cortisol level was analyzed by radioimmunoassay. Results①SA was significantly correlated with E, L, PC, N and NC, also TA was significantly correlated with E, L, PC, N, P and NC. ② N, PC and the NC had a good predictive efficiency on TA. ③NC was significantly higher in females than in males. Compared with an single-child, a non-single-child had significantly higher scores in SA and TA. ④ Saliva cortisol was not significantly different among different levels of TA. Conclusions① Anxiety levels, including SA/TA are significantly correlated with personality and coping style, and the N level has a more important impact on TA than others. ② Female students have more negative coping style and an single-child has more positive emotions. ③TA has no significant influences on the cortisol level.
    Improvement of the scoring system on human oocytes during maturation in vitro
    YANG Yong-jie,SUN Shu-yu,MA Shui-ying,JIANG Jing-jing,LI Mei,HU Jing-mei,LI Yuan
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  869-872. 
    Abstract ( 1165 )   PDF (443KB) ( 535 )   Save
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    To study the feasibility of the improved scoring system by evaluating oocytes cultured in vitro, and to discuss the relationship between developmental potential of human immature oocytes and their granulasa cells. Methods Cumulus enclosed oocyte complexes (COCs) were cultured, evaluated and fertilized. The IVM oocytes were morphologically classified into two groups based on the improved scoring standard. One group was of high quality and the other group was of poor quality. Granulosa cell apoptosis was detected by agarose gel electrophoresis. Results The immature oocyte scoring system was improved. The maturation rate，cleavage rate and high quality embryos of high quality oocytes were better than those of poor quality oocytes. DNA of granulosa cells distilled from poor quality oocytes was discovered as DNA ladder，while that from high quality ones was not. ConclusionThe improved scoring standard is feasible in clinical practice.
    Diagnosis value of DTI in cervical spondylosis in early stages
    MENG Xiang-shui,CHAI Xiang-jun,LI Du-min,MA Xiang-xing,LI Chuan-fu,HOU Jin-wen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  873-876. 
    Abstract ( 1111 )   PDF (286KB) ( 277 )   Save
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    To evaluate the diagnosis value of diffusion tensor imaging (DTI) in cervical spondylosis without high signal intensity on the compression sites. MethodsTwenty-one healthy volunteers and 42 patients with cervical spondylosis without high signal intensity on the T2WI were subjected to DTI by using a 3.0T MR scanner. Forty-two patients were divided into 2 groups based on having or not having cervical spondylosis symptoms. Patients in group A had symptoms of cervical spondylosis while patients in group B did not. ADC, FA, λ1, λ2 and λ3 values were analyzed with a software package of SPSS 11.0. Fiber tracking was performed on examinees with Functool software of the GE Company. ResultsHigh quality ADC maps, FA maps and fiber tracking of cervical spinal cords were obtained. For healthy subjects, average ADC, FA, λ1, λ2 and λ3 values were (0.78±0.08)×10-3mm2/s, 0.72±0.03, (1.51±0.15)×10-3mm2/s, (0.42±0.09)×10-3mm2/s and (0.41±0.10)×10-3mm2/s respectively. The FA value of group A was higher than that of group B（P＜0.01）, while values of ADC, FA, λ1, λ2 and λ3 of group B were higher than those of group A（P＜0.05）. The ADC, FA and λ1 values were not statistically significant between group A and the control group（P＞0.05）, while values of λ2 and λ3 of group A were higher than those of the control group（P＜0.05）. The FA value of the control group was higher than that of group B（P＜0.01）, and the ADC, λ2 and λ3 values of group B were higher than those of the control group （P＜0.05）, while there were no statistically significant differences between group B and the control group（P＞0.05）. ConclusionThe ADC, FA, λ1, λ2 and λ3 values are sensitive indices for evaluation of the subtle structure of cervical spinal cords which can be quantitatively analyzed by DTI in the early stage of cervical spondylosis.
    Association of the Y402H polymorphism in the complement factor H gene and neovascular age-related macular degeneration
    JI Xiang,QU Yi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  877-879. 
    Abstract ( 1078 )   PDF (391KB) ( 438 )   Save
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    To investigate the association between Y402H, in the complement factor H gene (CFH) and neovascular agerelated macular degeneration (AMD). Methods100 patients with neovascular AMD and 100 age-matched healthy controls in the clinic of Qilu Hospital were enrolled in this study. Genomic DNA from whole blood was extracted. The Y402H polymorphism in CFH was determined by polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP). The Y402H polymorphism in CFH showed a substitution of T to C at nucleotide position 1277 in exon 9. The association between genetic polymorphism and disease was examined by χ2 test and logistic regression. ResultsThe frequency of the risk allele 1277C was 14.0% in AMD patients compared with 5.0% in the controls (χ2=9.42，P<0.05)， mutational homozygote 1277CC in AMD cases was higher than that in the controls（χ2=9.08，P＜0.05）, and the difference between the two groups was statistically significant. ConclusionThe Y402H polymorphism in CFH is significantly associated with neovascular AMD and has an ethnic variation.
    Correlations of Syk with pathology and angiogenesis of pancreatic adenocarcinoma
    ZHANG Hong-yu,FEI Li-ming,WANG Chang-liang,ZHAO Wen-hua,ZHANG Bo,ZHOU Wu-yuan,ZHONG Wei-xia,LI Sheng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  880-883. 
    Abstract ( 961 )   PDF (540KB) ( 372 )   Save
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    To study the expression of Syk and its correlations with clinical pathology and angiogenesis in pancreatic adenocarcinoma (PAC). Methods Forty PAC, 9 chronic pancreatitis and 8 normal pancreatic tissues were collected. The expressions of Syk protein in these tissues were assayed by immunohistochemical staining, and also expressions of Syk mRNA in PAC were investigated by realtime PCR. MVD marked by CD34 was detected by morphometric analysis, and the relationship between MVD and clinical pathology of PAC was analyzed. ResultsThe positivity rate of Syk protein in PAC, chronic pancreatitis and normal pancreatic tissues was 27.5%, 77.8% and 100%, respectively. There were 29 samples with negative Syk protein, and among them the Syk levels had no relations to the cancer position, but had relations to the TNM classification, tumor differentiation and lymphotic metastasis（P＜0.05）. The MVD was higher in positive Syk proteins than in negative ones (P=0.000). ConclusionsThe expression of Syk in pancreatic cancer is significantly lower than that in chronic pancreatitis and normal pancreatic tissues and is related to the development of pancreatic cancer. MVD has significant relationship with differentiation and histology types of PAC.
    Serum estradiol and threatened abortion in pregnancy of 4 to 8 weeks
    GAO Qing,WANG Mei-hong,TANG Rong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  884-886. 
    Abstract ( 1814 )   PDF (245KB) ( 647 )   Save
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    To explore the relationship between serum estradiol and threatened abortion in women with pregnancy of 4 to 8 weeks. Methods259 pregnant women, who had conceived from 4 to 8 weeks were previously divided into 2 groups: 171 with normal pregnancy and 88 with threatened abortion, and then each group was divided into 5 subgroups based upon gestational age. Chemiluminescence reaction and dissociation enhanced lauthanide fluoroimmuno-assay were used to determine the serum esrtadiol levels. ResultsWith an increase of age, the level of serum E2 in women with normal pregnancy significantly increased (r=0.94, P<0.05), while, the serum E2 level in women with threatened abortion was significantly lower than that in normal pregnancy at the same gestational age. ConclusionThe serum level E2 is significantly decreased in women with threatened abortion, suggesting that serum E2 should be an important factor supporting early pregnancy.
    Bispectral index closedloop feed-back controlled system of volatile anesthetics
    WU Qi,WANG Huan-liang,LIU Rui-fang,ZHAO Lian-ying,LIU Xiao-ping,FAN Li-xia,WANG Cui-ping,LEI Wei-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  887-890. 
    Abstract ( 1020 )   PDF (762KB) ( 425 )   Save
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    To develop a closedloop feedback controlled volatile anesthesia system with index (BIS) as the control variable and to clinically validate it. MethodsIn the first stage, a closedloop feedback controlled volatile anesthesia system was set up. A volatile anesthetic physiological pharmacokinetics model was built in the system software, whose controlled algorithm was based on the proportional-integral-derivative principle. System software, anesthesia deep monitor, syringe pump and anesthesia machine made up the whole administrate system. In the second stage, enflurane, isoflurane or sevoflurane was used in clinical operations to verify the performance of the administrate system for patients with general surgeries, single side lung venting or obesity. ResultsThe close-loop feedback administrate system can maintain BIS around the target value and retained hemodynamics stabilization. This system reduced the inducement and analepsia time. All patients administrated by this system had not encountered awareness during general anesthesia. ConclusionThe system is able to provide clinically adequate anesthesia using the commonly used volatile anesthetics even when patients′ pharmacokinetics parameters have evident changes.
    The treadmill exercise test in diagnosis of cardiac neurosis
    LEI Lei,LIAN Xiu-hua,LI Wen-bin
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  891-893. 
    Abstract ( 1109 )   PDF (224KB) ( 417 )   Save
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    Objective To investigate the clinical significance of the chronctropic response index(CRI) during the treadmill exercise test in patients with cardiac neurosis. Methods40 patients who had been diagnosed cardiac neurosis received the treadmill test. Based on the test results, decrease of the ST segment, changes of the T wave and CRI were compared between the test group, the coronary heart disease (CHD) group and the control group. ResultsDuring the Treadmill test, the average CRI of 40 patients with cardiac neurosis was 1.41±0.05, which was obviously higher than that of the CHD and control groups（P<0.001）. In the test group, the ST segment was decreased by 0.12±0.04mV, which had no statistical differences from the CHD group but had a significant difference from the control group. Conclusion CRI in the cardiac neurosis group is significantly increased, and CRI＞1.3 conld be of significant value in diagnosis of cardiac neurosis.
    Force system intruding of two different procession attachment dentures
    QU Feng-jie,ZUO Shu-yu, SHI Yan-li,DAI Yan-ping,LIU Hong-shuang, MU Yue-zhao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  894-897. 
    Abstract ( 1137 )   PDF (1346KB) ( 529 )   Save
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    To explore the force system of different precision attachments in mandible distalextension edentulous by a finiteelement model. MethodsThe threedimensional finite element stress analysis method was used. Results The effects of attachments of ASC-52 were reduced in abutment when the molar was forced by 200N. When the molar was forced by 200N, the effection of the attachment ASC-52 was much more reduced than the attachment of the revax system. When the premolar was forced by 200N, the effection on attachments was reduced either liable or lingual. When the double molars were forced by 200?N, the stress intruding was added in mandibles and abutments. ConclusionsThe labial effection of abutments is smaller than lingual in the attachment of the revax system or ASC-52. The effection of the ASC-52 attachment is reduced in the abutment when the molars are forced.
    Concurrent chemoradiotherapy with docetaxel/cisplatin for local advanced non-small cell lung cancer
    JIA Zheng-fei,FENG Yong, ZHONG Qin,XIA Xiao-tian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  898-900. 
    Abstract ( 1060 )   PDF (250KB) ( 424 )   Save
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    To evaluate the efficacy and side effects of concurrent radiotherapy and chemotherapy with docetaxel/cisplatin for local advanced non-small cell lung cancer (NSCLC). Methods A total of 76 patients with stage Ⅲ NSCLC were randomly divided into two groups: the docetaxel/cisplatin chemotherapy concurrent radiotherapy group(concurrent group) and the docetaxel/cisplatin chemotherapy sequential radiotherapy group(sequential group). In the two groups, the radiation method was the same, five times a week at 2 Gy per day up to a total dose of 60-70Gy. Chemotherapy consisted of docetaxel 40mg/m2 d1，8 and cisplatin 20mg/d, on day 1 and 5, lasting 21 days. The concurrent group received radiotherapy on day 1 of chemotherapy, while the sequential group received radiotherapy after 2 chemotherapy cycles. ResultsThe response rate in the concurrent group was 65.7% and 41.5% in sequential group (P<0.05). The complete response rate was 17.1% in the concurrent group and 7.3% in sequential group (P>0.05). The acute toxic effects such as radiation esophagitis were more severe in the concurrent group than in the sequential group (P<0.05). ConclusionConcurrent radiotherapy and chemotherapy with docetaxel/cisplatin is an effective regimen for advanced nonsmall cell lung cancer with increased but tolerable toxic effects.
    Association between micro-albuminuria and endothelial dysfunction in aged patients with hypertension
    LI Chao,JIANG Bao-fa
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  901-903. 
    Abstract ( 1052 )   PDF (252KB) ( 405 )   Save
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    To explore the incidence of extrarenal vascular complications of hypertension in aged patients with micro-albuminuria (MA) and to investigate the relationship between the urinary albumin excretion rate and the endothelial dysfunction. MethodsAged patients with hypertension were divided into two groups: the control group(n=177)(UAER＜30?μg/min) and the MA group(UAER 30-200μg/min) （n=179）. Incidence of retinopathy, peripheral neuropathy, and lower extremity arterial sclerosis occlusion were determined, also the levels of plasma vonWillebrand factor (vWF) and endothelin-1 (ET-1) were determined. ResultThe incidences of retinopathy, peripheral neuropathy and lower extremity arterial sclerosis occlusion were significantly higher in the MA group than those in the control group (P＜0.05). The plasma vWF level was markedly increased in the MA group (202.07±73.30)% as compared with that in the control group (118.95±46.72)% (P＜0.01), also the plasma ET-1 level in the MA group (79.13±14.04) μg·L-1 was significantly higher than that in the control group (50.60±12.39) μg·L-1. The plasma vWF level was significantly correlated with UAER (r=0.72，P＜0.01) and the plasma level of ET-1 was closely associated with vWF (r=0.69，P＜0.01). ConclusionAged patients with hypertension with MA have more remarkable endothelial dysfunction than those without MA. The increase of UAER is correlated with the degree of endothelial dysfunction.
    Awake craniotomy in eloquent cortex surgery: a comparison of etomidate and propofol
    WU Jian-bo,XIAO Jian-min, WANG Ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  904-906. 
    Abstract ( 1185 )   PDF (277KB) ( 354 )   Save
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    To explore the effects of remifentanil combined with propofol or etomidate in providing conscious sedation for awake craniotomy in eloquent cortex surgery. MethodsThirty patients undergoning glioma removal in the cerebral cortical functional area were randomly divided into two groups: the etomidate group and the propofol group (n=15). The status of eloquent areas mapping, respiratory variables and hemodynamic stability were assessed, also the recall time and complications were documented. Results There were no differences in status of eloquent areas mapping or hemodynamic and respiratory variables between the two groups. Respiratory complications occurred in 1 patient in the propofol group, but there were significant differences in recall time between the two groups (etomidate, 321?s; propofol, 410?s). ConclusionEtomidate and propofol are good in the status of eloquent areas mapping, but etomidate is superior to propofol in recall time.
    Yinxingdamo combined with Lovastatin for acute cerebral infarction
    ZHAO Bin,MA Yong-chun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  907-909. 
    Abstract ( 1072 )   PDF (240KB) ( 586 )   Save
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    To explore the therapeutic effects of Yinxingdamo combined with Lovastatin in treatment of acute cerebral infarction. Methods 127 patients with acute cerebral infarction were randomly divided into three groups. The Chuanxiongqin group was subjected to Chuanxiongqin injection 200mg+Ns250mL, ivdrip, Qd, for 14 days; the Yinxingdamo group was subjected to Yinxingdamo injection 20mL+NS250mL, ivdrip, Qd, for 14 days; the combination group was subjected to the Yinxingdamo injection 20mL+NS250mL, ivdrip, Qd, and Lovastatin 40?mg, PO, QN. 14 days later, Yinxingdamo was disused and Lovastatin was continued. The therapeutic effects were evaluated after 30 days. ResultsThe response rate of the combination group was higher than that of the Yinxingdamo group（P＜0.05） and the Chuanxiongqin group（P＜0.01）. The degree of NFD and the levels of serum lipids and whole blood viscosity, and the plasma viscosity were significantly decreased（P＜0.01）. However, the HDLC level was significantly increased（P＜0.01）. ConclusionLovastatin combined with Yinxingdamo has obvious therapeutic effects on acute cerebral infarction, and it can improve the neurological function and decrease the levels of serum lipids and blood viscosity.
    Interventional therapy for benign strictures after esophagectomy
    JING Xue-hong,SUN Zeng-tao,CHEN Jie,GAO Li
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  910-912. 
    Abstract ( 953 )   PDF (333KB) ( 361 )   Save
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    To explore the safety and clinical curative effect of interventional therapy for postoperative benign esophageal strictures. MethodsInterventional therapies were performed on 32 patients with post-operative benign strictures, of them, balloon tympanoplasty was used in 26 cases 59 times(1 to 4 times per case ),and stent implantation in 6 cases with 8 retrievable metallic-covered stents. ResultsIn 26 cases of balloon dilatation, 4 cases were cured in 1 treatment, 13 cases in 2 treatments, 7 cases in 3 treatments and 2 cases in 4 treatments, and the constrictive manifestations were all improved. In 6 cases of stent implantation, stenosis above the stent was found in 4 cases. ConclusionBalloon dilatation combined with stent implantation is a safe and effective therapeutic method for postoperative benign esophageal strictures.
    Application of auto-fluorescence excited by endoscopic laser in the diagnosis of gastric cancer and its precancerosis
    WEI Hua-gang,ZHANG Ming-bao,JING Ming,XIE Li,GUO Jian-qiang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  913-914. 
    Abstract ( 1152 )   PDF (220KB) ( 420 )   Save
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    To study the value of laserinduced autofluorescence on the diagnosis of gastric cancer and its precancerosis. MethodsEndoscopic laserinduced fluorescence diagnosis spectra were taken in 198 patients with upper gastrointestinal symptom. Results198 patients were diagnosed by endoscopy and biopsy,including 20 gastric cancer, 22 gastric precancerosis,156 gastrelcoma and chronic superficial gastritis. Characteristic peaksof autofluorescence in cancerous tissue were observed in 16 out of 20 patients with gastric cancer and 19 out of 22patients with gastric precancerosis. The coincidence was 80.0% and 86.4% respectively. While 141 had no such peaks out of 156 patients with gastrelcoma and chronic superficial gastritis. ConclusionLaserinduced autofluorescence spectra have higher sensitivity and specificity in the diagnosis of gastric cancer and its precancerosis,and can be used as an effective means of supporting inspections.
    Prevalence rate of nosocomial infection in Qilu Hospital of Shandong University in 2007
    WU Shou-cai,WANG Shu-hui,WANG Shao-yan,LIU Yu,SUN Yu-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  915-918. 
    Abstract ( 1296 )   PDF (260KB) ( 596 )   Save
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    To investigate the present situation of nosocomial infection(NI) and the use of antibiotics and to provide scientific data for prevention and control of hospital infection. MethodsForms were filled with case data obtained from clinical investigations and references to inpatient medical records of all patients admitted to Qilu Hospital on 22th August 2007. ResultsAnalysis revealed that the prevalence rate of NI was 5.68% and the case prevalence rate was 5.93%, and the highest NI rate was found in the general ICU (36.36%). The respiratory tract was the main infected site (58.95%). The antibiotic adoption rate was 51.37%, and 59.30% of the antibiotics were singly used. Nine among 31 strains were identified as Escherichia Coli, 8 Pseudomonas aeruginosa, 2 Klebsiella pneumonia and 2 fungi. ConclusionsThe present NI rate survey indicates that targeted surveillance should be carried out in high risk departments, such as ICU, Department of Burns, Hemodia1ysis and Hematology, and it also helps doctors to properly use antibiotics to reduce the NI rate in the hospital.
    Correlation between subjective wellbeing and health status of rural residents
    WANG Jian,MENG Qing-yue,WINNIE Yip,JIA Li-ying,WANG Li-na
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  919-921. 
    Abstract ( 1102 )   PDF (318KB) ( 369 )   Save
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    To analyze the correlation between subjective wellbeing (SWB) and health status of rural residents. MethodSWB was measured using the Day Reconstruction Method (DRM) and health status was measured through the EQ5D scales. Results27% of investigated residents experienced an unhappy mood and felt unhappy in an average of 47% of their life. 31.9% and 17.9% residents felt pain or discomfort and anxiety or depression. Conclusion Subjective wellbeing is positively related to health status.
    Intake of energy nutrients and the nutritional status of patients with Alzheimer′s disease
    ZHAO Chang-feng,ZHANG Jun-li,WANG Shu-e,YU Hong-xia,YANG Xiao-dong,CHAO Hong,ZOU Ling-yan,ZHAO Yu-ping
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(9):  922-924. 
    Abstract ( 1039 )   PDF (250KB) ( 338 )   Save
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    To explore the intake of energy nutrients and the nutritional status of patients with Alzheimer′s disease and to explore their roles on Alzheimer′s disease. MethodsEighty-five patients with Alzheimer′s disease were selected as the case group and eightytwo health aged adults served as the control group. Basic information and intake of food in the two groups were investigated, data of body weight, body mass index (BMI) and body composition were detected by body composition analyzer (Biospace Inbody3.0), also triceps skinfold thickness (TST), upper arm circumference and lean weight were collected. ResultsIn the case group, average of BMI was 23.58±5.88, triceps skin-fold thickness (TST) was (17.24±3.65) cm, weight of the body fat was (16.41±2.41)kg and percentage of fat was (25.94%±3.79%), which were all significantly lower than those of the control group(P<0.05). Also intake of calories and protein per body weight kilogram per day were significantly higher in the case group than those in the control group (P<0.05), but there were no significant differences in intake of calories, protein, fat and carbohydrate per day in the two groups (P>0.05). ConclusionIntake of energy nutrients and nutritional status are influencing factors for Alzheimer′s disease.
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