JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Improvement of the scoring system on human oocytes during maturation in vitro

YANG Yong-jie1, SUN Shu-yu2, MA Shui-ying1, JIANG Jing-jing1, LI Mei1, HU Jing-mei1, LI Yuan1   

  1. Shandong University 1. Center for Human Reproduction of Shandong Provincial Hospital, Jinan 250021, China； 2. School Hospital, Jinan 250100, China

  • Received:2008-05-06 Revised:1900-01-01 Online:2008-09-16 Published:2008-09-16
  • Contact: LI Yuan

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Abstract: To study the feasibility of the improved scoring system by evaluating oocytes cultured in vitro, and to discuss the relationship between developmental potential of human immature oocytes and their granulasa cells. Methods Cumulus enclosed oocyte complexes (COCs) were cultured, evaluated and fertilized. The IVM oocytes were morphologically classified into two groups based on the improved scoring standard. One group was of high quality and the other group was of poor quality. Granulosa cell apoptosis was detected by agarose gel electrophoresis. Results The immature oocyte scoring system was improved. The maturation rate，cleavage rate and high quality embryos of high quality oocytes were better than those of poor quality oocytes. DNA of granulosa cells distilled from poor quality oocytes was discovered as DNA ladder，while that from high quality ones was not. ConclusionThe improved scoring standard is feasible in clinical practice.

Key words: Oocytes, In vitro maturation, Scoring system, Apoptosis

CLC Number: 

  • R713.7
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