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Table of Content

      
    16 October 2008
    Volume 46 Issue 10
    Articles
    Articles
    Changes of CD4+ CD25+ Foxp3+ and CD4+ CD25- Foxp3+ regulatory T cells in aging of mice
    GAO Qi,MA Tian,ZHANG Xia,JIANG Yang,ZHANG Li-ning
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  927-930. 
    Abstract ( 1038 )   PDF (1111KB) ( 430 )   Save
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    To explore the relationship between T cells and aging. Methods15 mice were divided into three groups: the 2-month (young), the 6 -month (middleaged) and the 15-month (aged) groups. Splenocyte suspensions were prepared in sterile conditions. Surface marks were stained with the PECy5-conjugated CD4 antibody and the FITC-conjugated CD25 antibody. After being washed with PBS, cells were treated with stiletto fluid and fixation fluid and stained with the PE-conjugated Foxp3 antibody. The proportion of CD4+ regulatory T cells was analyzed by flow cytometry. ResultsThe proportion of CD4+ T cells and CD4+ CD25+ /CD4+ T cells in splenocytes had no significant differences among the three groups. But the proportion of CD4+ Foxp3+ T cells was significantly elevated with an increase of age. The proportion of CD4+ Foxp3+ T cells was(16.83±0.44)% in aged mice, which was obviously higher than young and middle-aged mice（P＜0.01）, and the proportion of CD25+ Foxp3+ regulatory T cells gradually decreased. The proportion of CD25+ Foxp3+ T cells in the aged group or in middle-aged mice was lower than that in young mice（P＜0.05）.　However, the proportion of CD25- Foxp3+ regulatory T cells continuously elevated with an increase of age. Its proportion in aged mice was significantly higher than that in middle-aged mice(P＜0.01), and that in middle-aged mice was higher than that in young mice(P＜0.01). In addition, the ratio of CD4+ CD25+ Foxp3+ T to CD4+ CD25- Foxp3+ T cells showed a decreasing tendency with an increase of age. ConclusionThe proportion of CD4+ CD25+ Foxp3+ T cells shows an decreasing tendency with an increase of age, however, the proportion of CD4+ CD25- Foxp3+ T cells shows an increasing tendency. These suggest that the two regulatory subsets play different roles in aging.
    Effects of electric stimulation on recovery of motor function and expressions of Nogo-A and Nogo receptor after acute cerebral infarction in rats
    YANG Bing,ZHANG Xiu-qing,TANG Ji-you,SONG Yun,SUN Qin-jian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  931-935. 
    Abstract ( 961 )   PDF (583KB) ( 399 )   Save
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    To investigate the mechanisms of electric stimulation in improving neuro-rehabilitation by observing the effects of impaired limbs and bilateral electric stimulation on motor function and expressions of Nogo-A and Nogo receptor (NgR) following acute cerebral infarction in experimental rats. MethodsAdult male SpragueDawley (SD) rats were randomly divided into the normal (8 rats), sham operation, control, impaired limb and bilateral electric stimulation groups (each group had 32 rats). The acute cerebral infarction model was mimicked by using the middle cerebral artery occlusion (MCAO) technique. After cerebral infarction for 24h, the rats were treated with or without electric stimulation everyday according to different groups, followed by evaluation of the motor function and expression of Nogo-A and NgR in the border zone of cerebral infarction for 3, 7, 14 and 21 days by using the beam walking test (BWT) and immunohistochemistory. ResultsMotor function was significantly improved in the treatment groups with electric stimulation compared with the control group(P＜0.05). Also, the effect of the bilateral electric stimulation on recovery of motor function was better than that of the impaired limb treatment. Further studies demonstrated that the expressions of NogoA and NgR were remarkably increased in the control and treatment groups after cerebral infarction for 3 days (P＜0.05) in contrast to the sham operation group, and were decreased after 7 days and returned to the basal level following 21 days, whereas no significant point. When compared with the control group, the expressions of NogoA and NgR in the treatment groups were significantly reduced after cerebral infarction for 3 day, however, no significant differences were found after 21 days, in contrast to the impaired limb electric stimulation group, they were lower in the bilateral treatment group 7 days after cerebral infarction (P＜0.05) and returned to the same level of the impaired limb treatment group after 14 days. ConclusionsTreatment of acute cerebral infarction with electric stimulation, in a particular bilateral stimulation, can improve the movement of the paralyzed extremities and down-regulate the expressions of Nogo-A and NgR. Therefore, improvement of motor function by electric stimulation involves the inhibition of NogoA and NgR expressions after acute cerebral infarction.
    Apoptotic effects of ODC and AdoMetDC bi-antisense virus on esophageal cancer cell Eca109
    XU Jie,TIAN Hui,LIU Xian-xi,ZHANG Bing,LI Wen-jun,SONG Xu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  936-940. 
    Abstract ( 1053 )   PDF (954KB) ( 307 )   Save
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    To explore the inhibitory effects of Ad-ODC-AdoMetDCas on polyamine biosynthesis and esophageal cancer cell apoptosis. Methods Adenovirus-mediated gene transduction efficiency was assessed by counting the GFP-positive cells by using MTT. The malignant phenotype of the Eca109 cells was assessed by a growth curve. Western Blot and HPLC were used to determine the ODC and AdoMetDC expressions and polyamine content in Eca109 cells. TUNEL was used to analyze cell apoptosis. Morphology of the apoptotic cells was observed by an electron microscope. ResultApproximately 70% of the Eca109 cells were infected with Ad-ODC-AdoMetDCas when MOI reached 50. The expression of ODC was inhibited in the infected tumor cells. Ad-ODC-AdoMetDCas could inhibit the Eca109 cell growth and invasive ability. TUNEL proved that Ad-ODC-AdoMetDCas could lead to cell apoptosis. Under an electron microscope, chromatin condensation, nuclear disintegration and formation of apoptotic bodies were found. Conclusion Ad-ODC-AdoMetDCas has significant inhibitory effects on esophageal cancer cell proliferation, leads to cell apoptosis and has therapeutic potential for the treatment of esophageal cancer.
    Effect of octreotide combined with transpupillary thermotherapy on experimental choroidal neovascularization
    QU Yi,ZHANG Ying,ZHANG Xiao,ZHOU Fang,XU Xiao-yi
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  941-944. 
    Abstract ( 1153 )   PDF (577KB) ( 301 )   Save
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    To explore the possibility of only octreotide or octreotide combined with transpupillary thermotherapy (TTT) to inhibit choroidal neo-vascularization (CNV) in rat models. MethodsCNV was induced in adult rats with laser photoagulation. On postoperative day 7 PBS was retrobulbarly administered to 6 rats, octreotide 50μg/kg only to 6 rats, and octreotide 50?μg/kg combined with TTT to 6 rats. An additional 3 rats were not treated with laser or retrobulbar injection. On day 14 after photoagulation, fluorescein angiography was used to observe the fluorescein leakage, quantitative analysis on choroidal flatmounts was performed to evaluate the changes of CNV lesions, and quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) examinations were used to observe the changes of vascular endothelial growth factor (VEGF) and insulin-like growth factor 1(IGF-1) mRNA in RPE-choroidal complex. ResultsThe results demonstrated octreotide only or the combined therapy significantly inhibited fluorescein leakage on FA and the extent of neovascularization on choroidal flatmount of rat CNV models, and the VEGF and IGF1 mRNA levels were obviously decreased following both treatments (P<0.05). Combined administration was more efficient (P<0.05). ConclusionOctreotide combined with TTT may provide a potential new therapeutic strategy for CNV.
    Characteristics of microcosmic changes following tendon allograft transplantation preserved by two methods under light microscopy and transmission electron microscopy
    TENG Xue-ren,ZHAO Yong-sheng,HU Guang-liang,ZHOU Lun,LI Jian-min
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  945-950. 
    Abstract ( 987 )   PDF (1562KB) ( 309 )   Save
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    To observe the characteristics of microcosmic changes under light microscopy and transmission electron microscopy following tendon allografts transplantation. Methods Bone-Patella tendonBones (BPTB)of rabbits were kept with -80℃ deep-frozen or liquid nitrogen deepfrozen after programme freezing for 2 weeks. Microscopic changes with a light microscope and transmission electron microscope were observed 2 weeks after treatment of the BPTB grafts, and then, the cruciate ligament was reconstructed by an allograft. The microscopically anatomic changes of the grafts were observed with light microscopy and transmission electron microscopy at 3 weeks and 8 weeks. Results① The microscopic characteristics showed that the injury after program freezing and liquid nitrogen deepfrozen was slighter than that after the -80℃ deep-frozen method. ② The healing process and histological behavior of the tendon transplantation group were similar with those of the autografting group, however, which was better by liquid nitrogen after program freezing than by -80℃ deepfreezing. Conclusions Both methods can be used to preserve the allografts, and the liquid nitrogen deepfrozen after programme freezing method is better than the -80℃ deepfrozen method.
    Location of mesenchymal stem cells in human placentas: an experimental study
    MIAO Zong-ning,XU Qiu-lan,PAN Yu-hong,WANG Ling,ZHU Jian-zhong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  951-953. 
    Abstract ( 1154 )   PDF (388KB) ( 369 )   Save
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    To investigate the distribution pattern of mesenchymal stem cells (MSCs) in human placentas by immunofluorescence staining and immunohistochemical staining. MethodsFresh normal term placental tissues were taken from cesarean section without medical indication, and based on the central zone(A), the intermediate zone(B), the marginal zone(C), the chorionic plate layer(a), the middle layer(b) and the basal layer(c), they were divided into 9 groups: the Aa, Ab, Ac, Ba, Bb, Bc, Ca, Cb and Cc groups. Then, serial section, immunofluorescence staining and immunohistochemical staining were used to study the expressions of CD166, D44 and CD29. The HPIAS1000 colored image analysis system was used to analyze distribution of positive expression cells. ResultsCD166, CD44 and CD29 positive cells were mainly concentrated in the area under vascular endothelial cells and in the stroma surrounding the vessel. There were many strongly positive cells in the intermediate zone and the middle layer of the placenta (Ab) and few positive cells in the marginal zone(C). ConclusionCD166, CD44 and CD29 positive cells could be found in every position in human placentas, but were mainly distributed in the intermediate zone and the middle layer of the placenta (Ab).
    Effects of Pioglitazone on cognition function and AGEs-RAGE system in insulin resistance in rats
    LIU Xue-ping,ZHENG Min,HAO Yue-wei,HOU Liang,ZHANG Su-ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  954-958. 
    Abstract ( 1120 )   PDF (515KB) ( 415 )   Save
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    To explore the effects of Pioglitazone on cognition function and AGEs-RAGE system in insulin resistant rats. Methods 6-8week Wistar rats were fed with fructose to develop insulin resistant(IR) models for 4 weeks, then IR rat models were randomly divided into the IR group (n=13) and the Pioglitazone (PIO) group (n=13). Rats in the PIO group were given Pioglitazone 10?mg/(kg·d) by gavage for 12 weeks. The cognition ability of rats was assayed with the Morris water maze test. The expressions of advanced glycation end products were determined by immunofluorescence. Western blotting was used to detect the expression of PPARγ, Phospho -NF-κB and RAGE protein. Results① The Morris water maze test showed that escape latency was longer in the IR group and the PIO group than that in the normal control (NC) group (P＜0.01, P＜0.05), and it was shorter in the PIO group compared with the IR group(P＜0.01). ②　The expression of AGEs protein in the IR group and the PIO group was higher than that in the NC group, also it was significantly decreased in the PIO group compared with the IR group. ③　The expressions of RAGE and Phospho-NF-κB protein in the IR and PIO groups were higher than those in the NC group .Compared with the IR group, the expressions of RAGE and Phospho-NF-κB in the PIO group were significantly decreased. The expression of PPARγ protein in the IR and PIO groups was lower than in the NC group（P＜0.05）, however compared with the IR group, the expression of PPARγ in the PIO group was significantly increased（P＜0.05）. ConclusionThe strengthening of the AGEsRAGE system activation is a possible mechanism of brain damage. Pioglitazone therapy can ameliorate cognition function of IR rats which is probably related to its effect on decreasing the AGEs -RAGE system activation.
    Expression of TGFβR/Smads in endometrioid adenocarcinoma cancer cells and stromal cells
    WANG Fu-ling,HAN Xue-chuan,ZHAO Xing-bo,LI Ming-jiang,LI Ji-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  959-962. 
    Abstract ( 1104 )   PDF (445KB) ( 491 )   Save
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    To investigate the relationship between the status of the TGFβR/Smads signal pathway in cancer cells and in its stromal cells, and if this may affect the development of endometrioid adenocarcinoma. MethodsTGFβRⅠ, TGFβRⅡ, Smad2, Smad3, Smad4 and Smad7 were determined in hysteromyoma (12 cases) and endometrioid adenocarcinoma (18 cases) by the SP immunohistochemical method. ResultsTGFβRⅠ, TGFβRⅡ, Smad2, Smad3, Smad4 and Smad7 were found in hysteromyoma. TGFβR expression which was in positive correlation with Smads expression in normal endometrium cells(r=0.86, r=0.78). TGFβR/Smads expression disagreed in endometrial cancer cells and stromal cells and it was not found in stromal cells of endometrial adenocarcinoma per se. ConclusionsTGFβR/Smads signals exist in cells of normal endometrial proliferative /secretory phase but its pathway is defected in tumor stroma. To get the information about TGFβR/Smads signal way in stroma probably provides a neotarget for gene therapy of endometrioid carcinoma.
    Radial versus femoral approach for percutaneous coronary interventional procedures in the aged
    FENG Min,LI Ji-fu,ZHANG Mei,KONG Guang-ming,ZHANG Cheng,LI Gui-shuang,CHEN Yu-guo,LI Da-qing,YOU Bei-an,LI Chuan-bao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  963-965. 
    Abstract ( 1207 )   PDF (259KB) ( 354 )   Save
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    To explore the security and feasibility of percutaneous transradial artery approach for percutaneous coronary interventions in the aged. Methods196 patients over 70 years old with coronary artery diseases were subjected to coronary intervention treatment through radial(n=94) and femoral arteries(n=102) between September,　2005 and February, 2007. The success rate of operation, incidence rate of entry site complications, rate of discomfort occurrence and X-ray exposure time were compared between the two groups. ResultsThere were no differences in the success rate of operation and X-ray exposure time(P＞0.05), while the incidence rate of entry site complications and rate of discomfort occurrence in the radial artery group were lower than those in the femoral artery group（P＜0.05). ConclusionPercutaneous transradial artery approach is secure and feasible for coronary intervention treatment in the aged and has few vascular complications.
    Relationship between the plasma level of matrix metalloproteinase-9 and structural characteristics of plaques and percutaneous coronary intervention
    JIANG Hong-ju,LI Run-zhi, WANG Ying,XU Dong-mei,ZHANG Mei,ZHANG Yun,LI Ji-fu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  966-970. 
    Abstract ( 1089 )   PDF (283KB) ( 485 )   Save
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    To explore the plasma levels of MMP-9, M-SCF and CRP of patients with ACS and SA before and after percutaneous coronary intervention, so as to quantify the relation between structural characteristics of plaque and plasma levels of MMP-9, M-SCF and CRP. Methods35 patients with acute coronary syndrome and 27 patients with stable angina were enrolled in this study. Serum MMP-9, M-SCF and CRP levels were determined by the speed diffused light turbidimetric method and by enzyme association immunity adsorption law before and after percutaneous coronary intervention. Based on coronary angiography plaque morphology, the patch was typed as Ⅰ, Ⅱ and Ⅲ and based on ultrasound for the carotid artery, plaque was typed as stable and unstable plaques. Results ① Serum MMP-9, M-SCF and CRP levels were significantly higher in the ACS group than those in the SA group（P＜0.05). ② MMP-9, M-SCF and CRP levels were significantly different in the ACS and SA patients before and after intervention treatments（P＜0.05). ③ The majority of the coronary plaque of the ACS group was type Ⅱ and of the carotid artery plaque was vulnerable, which were significantly different from those of the SA group（P＜0.05). Conclusion Levels of MMP-9, M-SCF and CRP are related to plaque structural characteristics and plaque stability.
    Changes of heart rate variability and its relationship to cardiac size and function in children with myocarditis
    JIN You-peng,DENG Xin,WANG Yu-lin,HAN Bo,WANG Yi,HAN Xiu-zhen,MA Pei-ran
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  971-973. 
    Abstract ( 1204 )   PDF (251KB) ( 251 )   Save
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    To investigate the changes of heart rate variability (HRV) and its relationship to cardiac size and function in children with myocarditis. Methods100 children with myocarditis served as the experimental group and 50 normal children as the control group. All received holter ECG and color Doppler echocardiography examination, and the levels of serum creatine phosphokinase MB and troponin I were quantitatively determined. The 24hour HRV parameters included SDNN, SDANN, rMSSD, PNN50, LF, HF and LF/HF. Echocardiography parameters included end-diastolic diameter of left atrial (LAED), enddiastolic diameter of left ventricle (LVED), left ventricular ejection fraction (LVEF) and fractional shorting (LVFS). Linear regression was used to analyze the relationship between HRV and Echocardiography parameters. ResultsIn the experimental group, LF/HF, CKMB, cTnI and LAED were significantly higher(P<0.05) while SDNN, rMSSD, PNN50 and HF were significantly lower (P<0.05) compared with the control group. SDNN was moderately correlated with LAED(r=－0.52, P<0.05) and LVEF(r=0.49, P<0.05). ConclusionsIn children with myocarditis, both the vagal and sympathetic nerve are impaired. Some HRV parameters are correlated with abnormality of cardiac structure and function, while others are independent. Further study is needed to investigate the prognostic value of HRV in children with myocarditis.
    Effect of immunologic intervention for bronchiolitis in infancy
    刘向红,张岩
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  974-976. 
    Abstract ( 1025 )   PDF (253KB) ( 303 )   Save
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    To explore the effects of immunologic intervention on the Th1/Th2 immunologic derangement and relapse gasp in acute bronchiolitis. Methods50 infants less than 6 months with acute bronchiolitis were randomly divided into 2 groups: the experimental group and the control group. Meanwhile 15 normal infants served as the blank control. The control group was treated with routine therapies and the experimental group was treated with polysaccharide nucleic acid fraction of BCG for one year besides routine therapy. The levels of serum IL-10 and IL-12 were determined by using ELISA before and after the treatment. Also, times and duration of gasping were followed up for 2 years. ResultsThe IL-10 level was significantly higher in the experimental group than that in the blank control group, however the IL-12 level was significantly lower in the experimental group than that in the blank control group（P＜0.001). After polysaccharide nucleic acid fraction of BCG treatment, the IL10 level was significantly decreased and the IL-12 level was significantly increased（P＜0.01, also, the times of gasping in 2 years decreased（P＜0.001) and the duration of gasping was obviously shortened. Conclusion Immunologic intervention can improve the immunologic derangement of Th1/Th2 in bronchiolitis and prevents the development of asthma.
    Risk factors associated with the presence of spontaneous bacterial peritonitis in hepatitis B virus related liver cirrhosis
    LI Qiang,LIU Yue-jin, LI Wen-wen, YU Jin-hong, LIU Li, YANG Xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  977-979. 
    Abstract ( 946 )   PDF (268KB) ( 305 )   Save
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    To investigate the factors associated with the presence of spontaneous bacterial peritonitis (SBP) in patients with hepatitis B virus(HBV)related liver cirrhosis. Methods568 patients with HBV-related liver cirrhosis hospitalized in Jinan Infectious Disease Hospital were investigated. Demographic data and biochemical parameters were analyzed by using SPSS software. ResultsThe prevalence rate of SBP was 40.1% in the cohort studied.　Univariate analysis showed that the TBIL, PT and TG levels, and the thickness of the spleen were higher, however, the ALT, ALB, GGT, CH and PLT levels were lower in the SBP group than those in the nonSBP group. There were no statistical differences in gender, alcohol abuse, HBeAg, presence of diabetes, age, AST, AFP, AKP and diameter of portal vein levels between the two groups. After adjustment of gender, age, alcohol abuse, AKP abnormal, PLT abnormal, HBeAg, ALT, AST and diameter of the portal vein, multiple regression analysis showed that TBIL＞50μmol/L, ALB＜35g/L, CH＜2.6mmol/L and splenomegaly were independent risk factors of the presence of SBP. ConclusionsIncreased bilirubin, decreased albumin and cholesterol and splenomegaly are independent risk factors of the presence of SBP. Impaired liver function and splenomegaly, but not enlargement of portal vein diameter, are associated with the presence of SBP in HBV-related cirrhosis.
    Changes of circulation endothelial cells and HSP70 in hemorrhagic fever with renal syndrome
    ZHAO Xiu-hua,ZHANG Jing-yao,JING Yan,BIAN Peng-fei,WANG Chun-xia,CHEN Shi-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  980-983. 
    Abstract ( 1030 )   PDF (262KB) ( 339 )   Save
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    To explore the significance of changes of circulation endothelial cells（CECs）and of the expression of heat-shock protein70（HSP70） in patients with hemorrhagic fever with renal syndrome(HFRS). MethodFiftyfour HFRS patients were assigned to the mild group(20 cases), the moderate group(20 cases) and the severe group(14 cases), and 20 healthy volunteers served as the controls. After CECs were separated by using the isopyknic centrifugation method, the CEC number and HSP70 level were determined with flow cytometry. ResultsThe number of CEC and the expression of HSP70 of HFRS patients began to increase in the fever stage and maintained a high level in the hypotension and oliguria stages, then gradually decreased in the diuretic phase and the recovery phase. In patients with different clinical types of HFRS, the CEC number and the HSP70 level accordingly increased and reached the peak in severe and dangerous HFRS. In patients with different stages and types of HFRS, the number of CEC and expression of HSP70 were both significantly higher than those of the controls. ConclusionThe CEC number and expression of HSP70 both increase in HFRS patients and this is related to the stage and clinical type of HFRS.
    Effect of the semi-quantitative exercise test on the hemogram of healthy adults and its clinical implication
    HUANG Shan-shan,DONG Laidong,XU Cong-gao
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  984-987. 
    Abstract ( 1206 )   PDF (261KB) ( 513 )   Save
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    To explore the hemogram of healthy adults after the semiquantitative exercise test and to explore its potential significance. MethodsVenous blood samples were collected from healthy adults before （0min）and 5，10，15，20，25，60，120 and 180min after the exercise test and before（0min）and 5, 10, 15, 20 and 25?min after the epinephrine test on the second day. Complete blood was counted by an automated blood counter. Differences before and after tests were statistically analyzed. Results Five min after exercises, white blood cells （WBC）， neutrophils (NEUT)， lymphocytes (LYM) and platelets (PLT) elevated to their peak values in comparison with those before exercises（P<0.0001）. Ten min after exercise, red blood cell(RBC), hemoglobin(HGB) and hematocrit (HCT) elevated to their peak values in comparison with those before exercises（P=0.0164, P=0.0078，and P=0.0002). Ten min after the epinephrine test, WBC, NEUT， LYM and PLT were increased to their summit counts compared with the values before epinephrine（P<0.0001, P<0.001, P=0.01，and P=0.002）. However, RBC and other parameters had few changes. Conclusion After semiquantitative exercise tests and epinephrine tests, WBC, LYM and NEUT have similar timecount curves, so the semi0quantitative exercise test may replace the epinephrine test.
    Intravenous anesthesia with Remifentanil and Propofol in infants undergoing surgical repair of cleft lip
    ZHOU Chang-qing,ZHANG Li,WANG Xue-qin,DU Hong-mei
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  988-990. 
    Abstract ( 1081 )   PDF (254KB) ( 295 )   Save
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    To evaluate the efficacy and safety of intravenous anesthesia with remifentanil and propofol in infants undergoing surgical repair of cleft lip. MethodsIn total, 60 infants 3 to 9 monthold were enrolled and randomly received either a combination of remifentanil and propofol (R-P group, n=30)or a combination of sodium hydroxybutyrate and ketamine(γ-K group, n=30). Anesthesia was induced with remifentanil 1μg/kg， propofol 2mg/kg and succinylcholine chloride 1mg/kg or sodium hydroxybutyrate 80mg/kg，ketamine 1-2mg/kg and succinylcholine chloride 1mg/kg. Tracheal intubations were given after anesthesia induction and spontaneous respirations were retained. Anesthesia was maintained with remifentanil 0.05-0.1μg·kg-1·min-1 and propofol 4-6mg·kg-1·h-1 or ketamine 1-2mg/kg and sodium hydroxybutyrate. Respiratory rate, mean arterial blood pressure and heart rate changes were recorded at fixed intervals, also recovery characteristics and postoperative complications of the two groups were determined. ResultsRemifentanil in combination with propofol resulted in a lower mean arterial blood pressure, heart rate and recovery time and less postoperative side effects than sodium hydroxybutyrate in combination with ketamine. ConclusionThe combination of remifentanil and propofol is safe and effective with stable hemodynamics, rapid recovery and absence of side effects in infants undergoing surgical repair of cleft lip.
    Effects of Liposomal Prostaglandin E1 on the blood system in patients with cardiopulmonary bypass
    ZHANG Chun-yan,PAN Xiao-jun
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  991-993. 
    Abstract ( 1074 )   PDF (290KB) ( 255 )   Save
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    To explore the effects of Liposomal Prostaglandin E1(Lipo-PGE1) on the blood system in patients with cardiopulmonary bypass(CPB). Methods20 patients received cardiac valve replacement undergoing CPB were randomly divided into two groups (10 patients in each group): the experimental group and the control group. In the experimental group, patients were administered 3ng／(kg·min) Lipo-PGE1and Lipo-PGE1 which was added into the priming solution (5ng／mL) of the extracorporeal circulation machine. In the control group, patients were administered the same volume of normal saline. The levels of thromboxane B2(TXB2), 6-keto-Prostaglandin F1α(6-Keto-PGF1α) and free hemoglobin (F-HB) in the plasma were determined five times around CPB. ResultsIn the two groups, levels of TXB2, 6-Keto-PGF1α and F-HB were all obviously increased in CPB and they reached the highest when CPB stopped. The level of 6-Keto-PGF1α in the experimental group was significantly higher than that in the control group, while levels of TXB2, TXB2／6Keto-PGF1αand F-HB were significantly lower than those in the control group (P<0。05). Conclusion LipoPGE1 can reclaim the imbalance of TXA2／PGI2 in CPB and partly improves coagulation disorder and lessens hemolysis caused by CPB, so it can obviously reduce kidney injury.
    Effects of glycyrrhizae waterextraction on chronic renal failure and its mechanism in rats
    ZHANG Rui-bin,ZHU Bin,LI Jin,LI Su,WANG Xiao-ping,LIU Zi-dong
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  994-997. 
    Abstract ( 952 )   PDF (276KB) ( 276 )   Save
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    To investigate the effects of glycyrrhizae water-extraction on rats with chronic renal failure(CRF) and its mechanism. Methods30 Wistar rats were randomly divided into 3 groups: the sham operation group, the simple operation group and the therapy group, 10 rats in every group. On the 9th and 13th weeks, the 24h urine protein, urine creatinine and MDA levels were determined, the activities of Scr and SeGSHPx and the expression of AOPPs were determined, also the Ccr was calculated. Nephridial tissue homogenate was prepared and the activity of SeGSHPx, MDA and AOPPs were determined. Results24?h urine protein and Scr were obviously lower in the therapy group than those in the simple operation group(P＜0.05). Ccr and the activity of SeGSHPx of blood serum and nephridial tissue homogenate of the therapy group were obviously higher than those of the simple operation group (P＜0.05). The MDA levels of nephridial tissue homogenate and urine in the therapy group were obviously lower than those of the simple operation group(P＜0.05). Also, the AOPPs activities of blood serum and nephridial tissue homogenate in the therapy group were obviously lower than those of the simple operation group (P＜0.05). All indexes were significantly different among the three groups. ConclusionGlycyrrhizae waterextraction provides a protective effect on renal function in rats with CRF, which is related to its antioxidation reaction.
    Clinical features and central nerve imaging analysis in mitochondrial myopathy, encephalopathy, lactic acidosis and stroke-like episodes
    LI Hong-hao,LIU Shu-ping,YAN Chuan-zhu,LI Wei,ZHAO Yu-ying,WU Jin-ling,LI Da-nian
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  998-1001. 
    Abstract ( 1105 )   PDF (540KB) ( 314 )   Save
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    To explore the clinical features and neuro-imaging features in patients with mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke-like episodes. MethodsThe clinical manifestation and neuroimaging features of CT, MRI, MRA and MRS in 28 patients who were confirmed with MELAS syndrome by muscle biopsy were analyzed. ResultsThe clinical manifestations were migraine-like headache, seizures audiovisual deficit, strokes, cognitive regression and muscle weakness. Basal ganglia calcifications and low density lesions in the strokelike area were found by CT. Unilateral or bilateral multifocal lesions which had T1 and T2 prolongation with widening ventricles and cerebral atrophy were found by MRI, and they had no definite vascular territories but tended to fluctuate. The temporal and occipital lobes were most often involved and parietal and frontal lobes were secondary. Other parts such as thalami cerebellum and hippocampus can also be involved in which the cerebellum was most often. By MRS, a significant increase of Lac and a decrease of NAA were found, which was more significant in the centre area of the lesion than in the surrounding area. ConclusionMELAS syndrome can be diagnosed and differentially diagnosed based on features of clinical manifestations and neuroimaging, also it can be certified by muscle biopsies.
    Effects of photosensitizers on the tumor treated by laser
    Men Jin-e, LI Xiu-mei,JIN Hui,REN Hai-xia
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  1002-1003. 
    Abstract ( 1006 )   PDF (217KB) ( 305 )   Save
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    To explore the effect of photosensitizers on increasing the photosensitivity of tumors treated by laser. Methods Cell dynamics was used to determine the different effects of the four kinds of photosensitizers on tumor cells. Results All four photosensitizers were effective in increasing the photosensitivity and the effects of Chlorophyll, 019, Phthalocyanine Ester and PHD were in an ascending order. Conclusion Photosensitizers are useful in enhancing the use of lasers in killing and inhibiting tumor cells and may serve as references for treatment of tumors by lasers.
    Color Doppler ultrasound in the diagnosis of fetal portal vein malformations
    WANG Yan-qing,YU Guo-fang,MENG Jin-lai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  1004-1006. 
    Abstract ( 1177 )   PDF (419KB) ( 445 )   Save
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    To explore the role of color Doppler ultrasound in the diagnosis of fetal portal vein malformations. MethodsThe sonographic traits by colored Doppler ultrasound in 12 cases of portal vein malformations confirmed by autopsy or indirective portal vein angiography postnatal were reviewed and analyzed. Results4 cases had portal vein stenosis, 3 had absence of the portal vein, 2 had situs inversus of leftright portal vein, 2 had portal veininferior vena cava fistula, and 1 had hepatic arteryportal vein fistula. ConclusionColor Doppler ultrasound is valuable in the diagnosis of fetal portal vein malformations.
    A trend of mortality of pancreatic cancer during 1985-2004 in rural area of Zhaoyuan County
    DONG Ning-ning,WANG Nan-da,ZHANG Fa-xue,LI Wen-hai,DIAO Yu-tao,LI Hui-qing,WANG Ming-yu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  1007-1011. 
    Abstract ( 1171 )   PDF (527KB) ( 406 )   Save
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    To explore the trend of pancreatic cancer mortality based on the investigation from 1985 to 2004 in Zhaoyuan County. MethodMortality rate, constituent ratio, standardized mortality of the world population and the correlation coefficient were analyzed. ResultsFrom 1985 to 2004 the mortality rate of pancreatic cancer was 2.22/100,000, in which it was 2.86/100,000 for males and 1.58/100,000 for females. In the four periods of 1985-1989, 1990-1994, 1995-1999 and 2000-2004, mortality rates of pancreatic cancer dramatically increased, and increased by 100.59% in 2000-2004 compared with that in 1985-1989, and the standardized mortality rate was increased by 45.56%. The mortality rate of pancreatic cancer was strongly related to the mortality rate of liver cancer, but not to that of esophagus, stomach, intestine, breast and cervical cancer. ConclusionsIncidence rate of pancreatic cancer quickly increases in the rural area. Besides population aging, some unknown factors may be in function. Hepatitis virus may be associated with the incidence of pancreatic cancer.
    Mental health of rural left-behind children and their family cohesion and adaptability
    ZHAO Jie,LIN Yan-yan,CAO Guang-hai
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  1012-1015. 
    Abstract ( 1308 )   PDF (261KB) ( 540 )   Save
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    To study the situation of left-behind children′s mental health, family intimacy and adaptability. Methods500 left-behind children in the Jining area and the Linyi area were enrolled and MHT and FACESⅡ—CV questionnaires were used. ResultsThe statistics showed that left-behind children′s mental health varied based on gender. Children whose parents both worked outside behaved quite differently compared with those whose father or mother worked outside. Also, different guardians would exert different influences on the children. The key factors affecting leftbehind children′s family function were the state of parents′ going out for work, living conditions, gender and grade. Conclusion Left-behind children′s mental health is serious. The state of leftbehind children′s family cohesion and adaptability is not optimistic. The mental health of leftbehind children is closely related to family intimacy and adaptability.
    Determination of urinary tribendimidine metabolite-terephthalic acid by HPLC
    XU Jie,YUAN Gui-yan,WEI Chun-min,WANG Ben-jie,GUO Rui-chen
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2008, 46(10):  1016-1019. 
    Abstract ( 1091 )   PDF (494KB) ( 458 )   Save
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    To develop a HPLC method for determination of tribendimidine metabolite terephthalic acid (TPA) concentration in human urine. MethodsDiluted urine was separated on the Ultimate XB-C18 column (4.6mm×200mm, 5μm), eluted with methanol and 1% glacial acetic acid (35∶65, V/V) at 0.8mL/min and 30℃, and detected at 240nm with ferulic acid as an internal standard (IS). ResultsA good linearity was obtained from 1 to 400μg/mL (r=0.9954). The lower limit of quantitation (LLOQ) was 1μg/mL. The relative recoveries were (106.0±4.4)%, (107.4±4.2)% and (108.5±2.4)% at 3, 25 and 200μg/mL, and the intra- and inter-batch relative standard deviations (RSD) were less than 8% for all quality control samples. ConclusionThe developed HPLC method is proved to meet the requirements of biological sample analyses, and is appropriate for determination of urinary tribenmidine metaboliteTPA in human urine.
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