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Effect of the HSV-TK gene with adenovirus mediation on osteoarthritic synoviocytes

DING Yuan-jing, ZHANG Wei, LI Wei, ZHANG Lei, SUN Shui, WANG Jian, WANG Xian-quan   

  1. Department of Orthopaedic Surgery, Shandong Provincial Hospital of Shandong University, Jinan 250021, Shandong, China
  • Received:2006-11-15 Revised:1900-01-01 Online:2007-06-24 Published:2007-06-24
  • Contact: LI Wei

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Abstract: Objective: To investigate the effect of the HSVTK gene on adenovirusmediation on osteoarthritic synoviocytes. Methods: Cultured osteoarthritic synoviocytes were transduced with the adenovirus containing the HSV-TK gene. The expression of the adenovirus was determined. Cells without treatment served as the controls and the infected cells were treated with GCV. Then the inhibition ratio was determined. The preliminary morphological changes were observed by light microscopy and the nucleus subjected to fluorescent staining was observed by fluorescent microscopy. The proportion of necrosis and apoptosis was quantitatively analyzed. Results: The adenovirus had the ability to infect the cultured osteoarthritic synoviocytes. The proliferation of the treatment group was significantly inhibited (P<0.05). Necrosis and apoptosis were both found in transduced cells. Conclusion: The HSV-TK/GCV system significantly inhibits the proliferation of osteoarthritic synovial cells.

Key words: Simplexvirus, Thymidine kinase, Osteoarthritis, Gene therapy, Genes, structural, viral

CLC Number: 

  • R684.3
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