JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

• Articles • Previous Articles     Next Articles

Role of the SPK/S1P signal in the expression of VEGF in human hepatoma cells

JI Da-lei1，GAO Yan-jing1，FU Jin-dong1，SONG Jian-hua2，XU Jjian-min1   

  1. 1. Department of Gartroenterology, Qilu Hospital of Shandong University；2. Department of Radiation, Affiliated Hospital of Weifang Medical College
  • Received:2006-11-28 Revised:1900-01-01 Online:2007-03-24 Published:2007-03-24
  • Contact: GAO Yan-jing

PDF (PC)

1297

Abstract: Objective：To investigate the role of the SPK/S1P（sphingosine kinase/sphingosine-1-phospate） signal in regulating the expression of the vascular endothelial growth factor(VEGF) in human hepatoma cells. Methods: The human hepatoma cell line HepG2 was cultured in vitro. After treatment with a concentration of 5-20μmol/L DMS (dimethyl sphingosine) in culture medium，the VEGF protein levels at 12h were measured by an enzyme-linked immunosorbent assay(ELISA). Results: The expression of VEGF was obviously inhibited by DMS in a dose-dependent manner. Conclusion: DMS makes the VEGF expression decrease by inhibiting the SPK/S1P signal, suggesting that the SPK/S1P signal regulates the expression of VEGF in hepatoma cells.

Key words: Signal transduction, Carcinoma, hepatocellular, Endothelium, vascular, Growth substances

CLC Number: 

  • R735.7
[1] WANG Shao-Qing, LIU Yi-Hui, FU Ting-Ting, CHENG Jin-Yong, LIU Qiang. Classification of 31P MRS data for hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 42-46.
[2] LV Yan-Feng, DING Ke, WANG Jian-Xin, YU Hua-Long, BEI Shao-Sheng. Canceration of chronic anal fistula [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 55-57.
[3] HUANG Fang, KANG Rui, TUN Chun-Lin. Expressions of VEGF-C，nuclear factor-kappa B and  Survivin in nasopharyngeal carcinoma and their clinical implications [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 83-.
[4] YAN Peng, WANG Rong, DU Yi-Feng, SHEN Lun-Qian. AD7c-NTP level in urine of patients with Alzheimer′s disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 106-.
[5] YU De-Xin, CAEI Ti-Gong, MA Xiang-Xing, ZHANG Xiao-Ming, LI Chuan-Fu. Angiogenesis and maturation of hepatocellular carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 51-54.
[6] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[7] LI Yinglin, SONG Daoqing, XU Zhonghua. Identification of FKBP11 expression in clear cell renal cell carcinoma using bioinformatics analysis [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 45-51.
[8] MENG Zhaotun, LI Qin, SUN Hui, WU Xinfang, SUN Wenkai, GAO Wei. Correlation between interleukin-18 single nucleotide polymorphism and susceptibility of laryngeal squamous cell carcinoma: a report of 73 cases [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 58-63.
[9] LUO Xin, HE Bing, NIE Qingsheng, HOU Zhenbo, DONG Jun, LI Yuhua, ZENG Xiangqin, LIU Wei, KONG Demin, CAO Jinfeng. Comparison of the value of mono-exponential mode and diffusion kurtosis imaging mode in grading clear cell renal cell carcinoma using magnetic resonance diffusion-weighted imaging [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 89-95.
[10] DENG Qingwen, LIU Wenbin. Multilevel analysis of health related quality of life of patients with cardiovascular disease and its determinants [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 115-121.
[11] ZHAO Zuohui, LI Cuiling, WANG Daoguang, WANG Fengqin, QU Hongyi, DING Sentai, GONG Jing, LÜ Jiaju, YANG Jinghua. Effect of MnSOD acetylation on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 31-35.
[12] ZHU Lin, LI Weiwei, LIU Zhikai. Human stromal vascular fraction combined with adipose stem cells promotes the healing of radiation skin damage in nude mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 66-72.
[13] LI Xingkai, LIU Zhanye, JIANG Yunfeng, LI Jun. Clinicopathological factors and prognosis between primary central and peripheral lung squamous cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 73-78.
[14] FENG Zhiyu, YANG Yanliang, GUO Rui, XU Huihui, ZHANG Jian, GUO Kai, WANG Guangzhen, WANG Hui. Diagnosis and surgical treatment of pulmonary artery sling complicated with tracheal stenosis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 48-51.
[15] WANG Ningning, QU Linli, CAO Fangli, LIANG Lei. Application of computed tomography sagittal reconstruction for microwave ablation of hepatic carcinoma under the diaphragm [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(8): 71-75.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd