Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (2): 10-20.doi: 10.6040/j.issn.1671-7554.0.2024.0517

• Clinical Medicine • Previous Articles     Next Articles

Correlation analysis of combining ACE, KLK1 and PTGIS genotypes among 250 NSTE-ACS patients

HOU Xiaohui, AREZOU Bikdeli, MA Chao, LI Daqing   

  1. State Key Laboratory for Innovation and Transformation of Luobing Theory;
    Key Laboratory of Cardiovascular Remodeling and Function Research, Ministry of Education, National Health Commission, Chinese Academy of Medical Sciences and Shandong Province;
    Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Online:2025-03-10 Published:2025-03-07

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Abstract: Objective To investigate the association of ACE insertion/deletion, kallilrein gene(KLK)1(rs5517), prostacyclin synthase gene(PTGIS)(rs5629)locus polymorphisms with susceptibility and degree of coronary artery disease for 250 patients with non ST segment elevation acute coronary syndrome(NSTE-ACS). Methods Clinical data of 200 patients with coronary artery disease and 50 patients with normal coronary arteries were collected and genotyped by PCR and Sanger sequencing, respectively. Susceptibility of the three genotypes and mutual-combination genotypes associated with NSTE-ACS were analyzed by binary Logistic regression using case-control groupings. Gensini score and SYNTAX score were used to express the degree of coronary artery stenosis, and multiple linear regression was used to analyze the association between the mutual-combination genotypes and the severity of coronary artery disease. Results In binary Logistic regression analysis, after adjusting for confounding factors such as age, LDL, and homocysteine, the genotypes associated with the risk of NSTE-ACS were ACE DD(OR=4.335, 95%CI: 1.105-17.016, P=0.036), KLK1 CC(OR=3.152, 95%CI: 1.077-9.230, P=0.036), and KLK1 TT& PTGIS TT(OR=0.065, 95%CI: 0.006-0.752, P=0.029). In multiple linear regression analysis, the combined genotype associated with Gensini score was ACE DD&KLK1 CC(β= 51.847, P=0.001), and the combined genotype associated with SYNTAX score was ACE DD&KLK1 CC(β=10.031, P=0.001). Conclusion ACE I/D genotype and KLK1(rs5517)genotype are associated with NSTE-ACS, and ACE DD genotype and KLK1 CC genotypes increase the risk of NSTE-ACS; KLK1 TT& PTGIS TT subtype may reduce the risk of NSTE-ACS patients of Han nationality in Shandong Province. ACE DD & KLK1 CC subtype is positively associated with the severity of coronary artery disease.

Key words: ACE insertion/deletion, Kallilrein gene 1, Prostacyclin synthase gene, Non ST segment elevation acute coronary syndrome, Single nucleotide polymorphism, Coronary artery stenosis

CLC Number: 

  • R541.4
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