Journal of Shandong University (Health Sciences) ›› 2024, Vol. 62 ›› Issue (12): 96-101.doi: 10.6040/j.issn.1671-7554.0.2024.1010

• Clinical Medicine • Previous Articles    

Role of PAR-2 during Aspergillus fumigatus infection in human corneal epithelial cells

NIU Yawen, LI Fengjiao   

  1. Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, Shandong China
  • Published:2024-12-09

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Abstract: Objective To investigate the expression and function of protease activated receptor-2(PAR-2)in human corneal epithelial cells(HCECs)stimulated with Aspergillus fumigatus(A. fumigatus). Methods 75% ethanol-killed A. fumigatus stimulated HCECs, and PAR-2 mRNA and protein expressions were tested by RT-qPCR and Western blotting. HCECs were stimulated with 75% ethanol-killed A. fumigatus with or without pretreatment of FSLLRY-NH2, PCR, Western blotting, and ELISA tested the expressions of IL-1β, TNF-α and p-p38. Results In HCECs, PAR-2 mRNA and protein expressions were significantly increased by A. fumigatus. A. fumigatus upregulated IL-1β, TNF-α and p-p38 expressions levels. FSLLRY-NH2 significantly inhibited IL-1β, TNF-α and p-p38 expressions compared with infected control. Conclusion A. fumigates stimulation increases PAR-2 expression and upregulates cytokine expression levels though the PAR-2/p-p38 pathway.

Key words: Protease activated receptor-2, Corneal epithelial cells, Aspergillus fumigatus, Cytokines, Signal pathway

CLC Number: 

  • R772.2
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