Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (12): 7-12.doi: 10.6040/j.issn.1671-7554.0.2022.0736

• 基础医学 • Previous Articles    

Mechanism of hydrogen sulfide in improving atrial fibrillation induced by obstructive sleep apnea based on oxidative stress

ZHAO Yaqing1,2, XU Jingwen2, WANG Xiao2, HOU Yinglong2, GAO Mei1,2   

  1. 1. The Second Clinical College of Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong, China;
    2. Department of Cardiology, The First Affiliated Hospital of Shandong First Medical University &
    Shandong Provincial Qianfoshan Hospital, Shandong Medicine and Health Key Laboratory of Cardiac Electrophysiology and Arrhythmia, Jinan 250014, Shandong, China
  • Published:2022-12-01

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Abstract: Objective To investigate the mechanism of exogenous donor of hydrogen sulfide, NaHS, in the treatment of atrial fibrillation(AF)in rats with obstructive sleep apnea(OSA)induced oxidative stress. Methods A total of 24 male SD rats were randomly divided into control, model and NaHS groups, with 8 rats in each group. All rats were intubated under anesthesia. The model and NaHS groups simulated OSA process by circulating air holding method. The electrophysiological process of the heart was recorded throughout the experiment. Blood and left atrial tissue were collected after the experiment. The content of malondialdehyde was determined with thiobarbituric acid method. The activity of superoxide dismutase was detected with xanthine oxidase method. The expressions of oxidative stress indexes NADPH oxidase 4(Nox4)and connexin 43(Cx43)were detected with Western blotting. Results Compared with the model group, the NaHS group had reduced induction rate and duration of AF(P<0.05). Compared with the control group, the model group had increased expression of Nox4 and malondialdehyde, decreased superoxide dismutase activity, and decreased expression of Cx43. After NaHS intervention, Nox4 expression was inhibited, malondialdehyde content was decreased, superoxide dismutase activity was increased, and Cx43 expression was increased(P<0.05). Conclusion Hydrogen sulfide can reduce the oxidative stress injury of AF induced by OSA, thus reducing the occurrence and duration of AF. The mechanism is closely related to the inhibiting of Nox4 activation, scavenging of oxygen free radicals, inhibiting of lipid peroxidation, and up-regulating of Cx43 expression.

Key words: Atrial fibrillation, Obstructive sleep apnea, Hydrogen sulfide, NADPH oxidase 4

CLC Number: 

  • R541.7
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