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H3K27 dimethylation changes during BMDLSCs differentiation into hepatocytes from rats in vitro

LI Guanhong1, WANG Wei1, LI Jiye1, A Sileng2, PAN Runhua1, LIAO Caixian1   

  1. 1. Department of Hepatobiliary Surgery, Nanfang Hospital, Nanfang University, Guangzhou 510515, Guangdong, China;
    2. Department of General Surgery, Affiliated Hospital of Inner Mongolia Medical University,
    Hohhot 010050, Inner Mongolia, China
  • Received:2014-01-23 Online:2014-06-10 Published:2014-06-10

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Abstract: Objective  To explore the changes of H3K27 dimethylation during the differentiation of bone marrow-derived liver stem cells (BMDLSC) into hepatocytes from rats in vitro and to investigate the relationship between them. Methods  BMDLSCs collected were isolated from rat bone marrow using a magnetic activated cell sorting (MACS). Purified BMDLSCs were induced into hepatocytes in vitro. The expressions of albumin (ALB) and alpha fetoprotein (AFP) were detected dynamically by RT-PCR. The changes of histone H3K27 dimethylation were measured by Western blotting and immunofluorescence staining on day 0, 7, and 14. Results  The expression of ALB gradually increased when BMDLSCs were induced in vitro, while AFP expression firstly rose and later fell. H3K27 from fresh BMDLSCs showed no significant methylation at first, but on day 7 after differentiation, the level of H3K27 dimethylation significantly increased and further rose on day 14.  Conclusion  The level of dimethylation shows a gradual increase during the differentiation of BMDLSCs. H3K27 dimethylation may regulate the differentiation of BMDLSC into hepatocytes.

Key words: Bone marrow-derived liver stem cell, Differentiation, Hepatocyte, H3K27me2

CLC Number: 

  • R34
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