Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (7): 102-108.doi: 10.6040/j.issn.1671-7554.0.2024.1011

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Epidemiological and genotyping characteristics of human rhinovirus among influenza-like illness in children in Jinan from March 2019 to February 2024

DING Xiaoman1,2, LYU Yan1,2, SUN Jiawen1,2, ZHAO Baotian1,2, QI Na1,2, JIAO Haitao1,2   

  1. 1. Institute of Microbiology, Jinan Municipal Center for Disease Control and Prevention, Jinan 250021, Shandong, China;
    2. Institute of Microbiology, Jinan Municipal Center for Disease Control and Prevention Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Published:2025-07-08

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Abstract: Objective To investigate the prevalence characters and genotypes of human rhinovirus(HRV)among influenza-like illness(ILI)in children in Jinan from March 2019 to February 2024, and to provide prevention and control strategies for HRV infection in children. Methods The nasopharyngeal swab samples from ILI in children aged 0 to 14 years were collected weekly in a childrens hospital in Jinan. Multi-pathogen nucleic acid detection was conducted by real-time fluorescence quantitative polymerase chain reaction(RT-PCR). Some nucleic acid positive samples of HRV were used in amplification and sequencing of the VP4/VP2 gene segments. A phylogenetic tree was constructed and homology analysis was performed. Results Respiratory pathogens were positive in 645 of 1,200 specimens, accounting for 53.75%. HRV had the 2nd highest detection rate after influenza virus at 7.67%(92/1,200), of which the positive rates of male and female cases were 8.02%(57/711)and 7.16%(35/489), respectively, with no statistically significant difference(χ2=0.001,P>0.05). The age distribution of the preschool(3 to <6 years old)age group was the highest(8.90%), and the difference in the positive rates of various age groups was not statistically significant(χ2=1.92,P>0.05). The positive rates of HRV in different monitoring years and seasons were statistically significant(χ2=18.67,P=0.001; χ2=35.88,P<0.001). The VP4/VP2 region sequences of the 28 HRV strains were compared with the reference strain and analyzed, and two genotypes(including 21 serotypes)were involved, with HRV A group as the main group, followed by HRV C group, and HRV B group genotypes were not detected. Conclusion From March 2019 to February 2024, the HRV positivity rate among children with ILI in Jinan City was similar in age distribution and different in seasonal distribution. The prevalence of HRV was only briefly suppressed and then rapidly rebounded. In addition, HRV A was predominant, followed by HRV C and multiple serotypes were involved in Jinan during this period.

Key words: Human rhinovirus, Influenza-like illness, Epidemiological characteristics, Phylogenetic tree, Homology analysis

CLC Number: 

  • R183.3
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