Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (10): 57-61.doi: 10.6040/j.issn.1671-7554.0.2022.0876

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Ultrasound-guided fine needle aspiration biopsy and BRAFV600E molecular detection in the diagnosis of thyroid cancer

LIU Yan, CAO Guanglei, CHEN Li   

  1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-30

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Abstract: Objective To investigate the value of ultrasound-guided fine needle aspiration biopsy(FNAB)and molecular detection of v-raf murine sarcoma viral oncogenic homolog B1(BRAF)V600E(BRAFV600E)in the diagnosis of thyroid carcinoma. Methods This study retrospectively analyzed 304 patients with thyroid nodules during Jan. 2019 and Dec. 2021. FNAB cytopathology and BRAFV600E molecular testing were performed. With postoperative pathological diagnosis as the gold standard, the patients were divided into FNAB group(n=138)and FNAB combined with BRAFV600E group(n=166). The receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to evaluate the diagnostic efficacy of the two groups. The ultrasound characteristics of Bethseda I and III-V nodules were also analyzed. Results Among the 304 patients, 278 were diagnosed with thyroid carcinoma, including 277 papillary thyroid cancer, 1 medullary thyroid cancer, and 26 benign lesions. There were 53 nodules with uncertain cell diagnosis, and the characteristics of ultrasonography were calcification(28.3%)and nodular size < 1 cm(45.3%)was dominant. According to the postoperative pathological results, efficacy evaluation of FNAB group was as follows: the sensitivity, specificity, and AUC were 93.9%, 57.1% and 0.824, respectively. Efficacy evaluation of FNAB combined with BRAFV600E group was as follows: the sensitivity, specificity, and AUC were 98.0%, 84.2% and 0.911, respectively. And AUC combined analysis> AUCFNAB. Conclusion The diagnostic efficiency of FNAB combined with BRAFV600E is higher than that of FNAB alone.

Key words: Fine needle aspiration biopsy, V-raf murine sarcoma viral oncogene homolog B1, Thyroid carcinoma, Cytopathology, Thyroid nodule

CLC Number: 

  • R581
[1] Grani G, Sponziello M, Pecce V, et al. Contemporary thyroid nodule evaluation and management [J]. J Clin Endocrinol Metab, 2020, 105(9): 2869-2952.
[2] Sajisevi M, Caulley L, Eskander A, et al. Evaluating the rising incidence of thyroid cancer and thyroid nodule detection modes: a multinational, multi-institutional analysis [J]. JAMA Otolaryngol Head Neck Surg, 2022, 4: e221743. doi: 10.1001/jamaoto.
[3] Ahmadi S, Pappa T, Kang AS, et al. Point of care measurement of body mass index and thyroid nodule malignancy risk assessment [J]. Frontiers in endocrinology, 2022, 13: 824226. doi: 10.3389/fendo.2022.824226.
[4] Chen B, Xie Z, Duan X. Thyroid cancer incidence trend and association with obesity, physical activity in the United States [J]. BMC Public Health, 2022, 22(1): 1333-1345.
[5] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015 [J]. CA Cancer J Clin, 2016, 66(2): 115-132.
[6] Kim J, Gosnell E, Roman SA. Geographic influences in the global rise of thyroid cancer [J]. J Clin Endocrinol Metab, 2020, 16(1): 17-29.
[7] Kitahara CM, Sosa JA. The changing incidence of thyroid cancer [J]. Nat Rev Endocrinol, 2016, 12(11): 646-653.
[8] Livhits M, Zhu C, Kuo E, et al. Effectiveness of molecular testing techniques for diagnosis of indeterminate thyroid nodules: a randomized clinical trial [J]. JAMA Oncol, 2021, 7(1): 70-77.
[9] 张旭东, 陈瑞雪, 王洁. BRAF基因突变与肿瘤[J]. 中国细胞生物学学报, 2017, 39(5): 668-674. ZHANG Xudong, CHEN Ruixue, WANG Jie. BRAF gene mutation in tumor [J]. Chinese Journal of Cell Biology, 2017, 39(5): 668-674.
[10] Abdullah M, Junit S, Ng K, et al. Papillary thyroid cancer: genetic alterations and molecular biomarker investigations [J]. Int J Med Sci, 2019, 16(3): 450-460.
[11] Cibas E, Ali SZ. The Bethesda system for reporting thyroid cytopathology [J]. Thyroid, 2009, 19(11): 1159-1165.
[12] Miranda-Filho A, Lortet-Tieulent J, Bray F, Cao B, et al. Thyroid cancer incidence trends by histology in 25 countries: a population-based study [J]. Lancet Diabetes Endocrinol, 2021, 9(4): 225-234.
[13] Sciuto R, Romano L, Rea S, et al. Natural history and clinical outcome of differentiated thyroid carcinoma: a retrospective analysis of 1503 patients treated at a single institution [J]. Ann Oncol, 2009, 20(10): 1728-1735.
[14] Collet JF, Lacave R, Hugonin S, et al. BRAF V600E detection in cytological thyroid samples: a key component of the decision tree for surgical treatment of papillary thyroid carcinoma [J]. Head Neck, 2016, 38(7): 1017-1021.
[15] Hay ID, McConahey WM, Goellner JR. Managing patients with papillary thyroid carcinoma: insights gained from the Mayo Clinics experience of treating 2,512 consecutive patients during 1940 through 2000[J]. Trans Am Clin Climatol Assoc, 2002, 113: 241-260.
[16] 杨粒芝, 孙霄, 商蒙蒙, 等. 基于中国版甲状腺影像报告与数据系统的甲状腺结节恶性风险预测模型[J]. 山东大学学报(医学版), 2022, 60(6): 64-69. YANG Lizhi, SUN Xiao, SHANG Mengmeng, et al. C-TIRADS-based nomogram for malignant risk prediction of thyroid nodules [J]. Journal of Shandong University(Health Sciences), 2022, 60(6): 64-69.
[17] Haugen BR, Alexander EK, Bible KC, et al. 2015 American thyroid association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American thyroid association guidelines task force on thyroid nodules and differentiated thyroid cancer [J]. Thyroid, 2016, 26(1): 1-133.
[18] Alexander EK, Doherty GM, Barletta JA. Management of thyroid nodules [J]. Lancet Diabetes Endocrinol, 2022, 10(7): 540-548.
[19] Alexander EK, Doherty GM, Barletta JA. Diagnosis of thyroid nodules [J]. Lancet Diabetes Endocrinol, 2022, 10(7): 533-539.
[20] Xing M, Tufano RP, Tufaro AP, et al. Detection of BRAF mutation on fine needle aspiration biopsy specimens: a new diagnostic tool for papillary thyroid cancer [J]. J Clin Endocrinol Metab, 2004, 89(6): 2867-2872.
[21] Xing M, Alzahrani AS, Carson KA, et al. Association between BRAF V600E mutation and mortality in patients with papillary thyroid cancer [J]. JAMA, 2013, 309(14): 1493-1501.
[22] Poulikakos PI, Sullivan RJ, Yaeger R. Molecular pathways and mechanisms of BRAF in cancer therapy [J]. Clin Cancer Res, 2022, 29: clincanres.2138.2021. doi: 10.1158/1078-0432.CCR-21-2138.
[23] Nikiforov YE, Nikiforova MN. Molecular genetics and diagnosis of thyroid cancer [J]. Nat Rev Endocrinol, 2011, 7(10): 569-580.
[24] Grogan RH, Mitmaker EJ, Clark OH. The evolution of biomarkers in thyroid cancer-from mass screening to a personalized biosignature [J]. Cancers(Basel), 2010, 2(2): 885-912.
[25] Li C, Lee KC, Schneider EB, et al. BRAF V600E mutation and its association with clinicopathological features of papillary thyroid cancer: a meta-analysis [J]. J Clin Endocrinol Metab, 2012, 97(12): 4559-4570.
[26] Zatelli MC, Trasforini G, Leoni S, et al. BRAF V600E mutation analysis increases diagnostic accuracy for papillary thyroid carcinoma in fine-needle aspiration biopsies [J]. Eur J Endocrinol, 2009, 161(3): 467-473.
[27] Kim SW, Lee JI, Kim JW, et al. BRAFV600E mutation analysis in fine-needle aspiration cytology specimens for evaluation of thyroid nodule: a large series in a BRAFV600E-prevalent population [J]. J Clin Endocrinol Metab, 2010, 95(8): 3693-3700.
[1] YANG Lizhi, SUN Xiao, SHANG Mengmeng, GUO Lu, SHI Dandan, LI Jie. C-TIRADS-based nomogram for malignant risk prediction of thyroid nodules [J]. Journal of Shandong University (Health Sciences), 2022, 60(6): 64-69.
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