Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (10): 57-61.doi: 10.6040/j.issn.1671-7554.0.2022.0876

Previous Articles     Next Articles

Ultrasound-guided fine needle aspiration biopsy and BRAFV600E molecular detection in the diagnosis of thyroid cancer

LIU Yan, CAO Guanglei, CHEN Li   

  1. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-30

PDF (PC)

43

Abstract: Objective To investigate the value of ultrasound-guided fine needle aspiration biopsy(FNAB)and molecular detection of v-raf murine sarcoma viral oncogenic homolog B1(BRAF)V600E(BRAFV600E)in the diagnosis of thyroid carcinoma. Methods This study retrospectively analyzed 304 patients with thyroid nodules during Jan. 2019 and Dec. 2021. FNAB cytopathology and BRAFV600E molecular testing were performed. With postoperative pathological diagnosis as the gold standard, the patients were divided into FNAB group(n=138)and FNAB combined with BRAFV600E group(n=166). The receiver operating characteristic(ROC)curve and area under the curve(AUC)were used to evaluate the diagnostic efficacy of the two groups. The ultrasound characteristics of Bethseda I and III-V nodules were also analyzed. Results Among the 304 patients, 278 were diagnosed with thyroid carcinoma, including 277 papillary thyroid cancer, 1 medullary thyroid cancer, and 26 benign lesions. There were 53 nodules with uncertain cell diagnosis, and the characteristics of ultrasonography were calcification(28.3%)and nodular size < 1 cm(45.3%)was dominant. According to the postoperative pathological results, efficacy evaluation of FNAB group was as follows: the sensitivity, specificity, and AUC were 93.9%, 57.1% and 0.824, respectively. Efficacy evaluation of FNAB combined with BRAFV600E group was as follows: the sensitivity, specificity, and AUC were 98.0%, 84.2% and 0.911, respectively. And AUC combined analysis> AUCFNAB. Conclusion The diagnostic efficiency of FNAB combined with BRAFV600E is higher than that of FNAB alone.

Key words: Fine needle aspiration biopsy, V-raf murine sarcoma viral oncogene homolog B1, Thyroid carcinoma, Cytopathology, Thyroid nodule

CLC Number: 

  • R581
[1] Grani G, Sponziello M, Pecce V, et al. Contemporary thyroid nodule evaluation and management [J]. J Clin Endocrinol Metab, 2020, 105(9): 2869-2952.
[2] Sajisevi M, Caulley L, Eskander A, et al. Evaluating the rising incidence of thyroid cancer and thyroid nodule detection modes: a multinational, multi-institutional analysis [J]. JAMA Otolaryngol Head Neck Surg, 2022, 4: e221743. doi: 10.1001/jamaoto.
[3] Ahmadi S, Pappa T, Kang AS, et al. Point of care measurement of body mass index and thyroid nodule malignancy risk assessment [J]. Frontiers in endocrinology, 2022, 13: 824226. doi: 10.3389/fendo.2022.824226.
[4] Chen B, Xie Z, Duan X. Thyroid cancer incidence trend and association with obesity, physical activity in the United States [J]. BMC Public Health, 2022, 22(1): 1333-1345.
[5] Chen W, Zheng R, Baade PD, et al. Cancer statistics in China, 2015 [J]. CA Cancer J Clin, 2016, 66(2): 115-132.
[6] Kim J, Gosnell E, Roman SA. Geographic influences in the global rise of thyroid cancer [J]. J Clin Endocrinol Metab, 2020, 16(1): 17-29.
[7] Kitahara CM, Sosa JA. The changing incidence of thyroid cancer [J]. Nat Rev Endocrinol, 2016, 12(11): 646-653.
[8] Livhits M, Zhu C, Kuo E, et al. Effectiveness of molecular testing techniques for diagnosis of indeterminate thyroid nodules: a randomized clinical trial [J]. JAMA Oncol, 2021, 7(1): 70-77.
[9] 张旭东, 陈瑞雪, 王洁. BRAF基因突变与肿瘤[J]. 中国细胞生物学学报, 2017, 39(5): 668-674. ZHANG Xudong, CHEN Ruixue, WANG Jie. BRAF gene mutation in tumor [J]. Chinese Journal of Cell Biology, 2017, 39(5): 668-674.
[10] Abdullah M, Junit S, Ng K, et al. Papillary thyroid cancer: genetic alterations and molecular biomarker investigations [J]. Int J Med Sci, 2019, 16(3): 450-460.
[11] Cibas E, Ali SZ. The Bethesda system for reporting thyroid cytopathology [J]. Thyroid, 2009, 19(11): 1159-1165.
[12] Miranda-Filho A, Lortet-Tieulent J, Bray F, Cao B, et al. Thyroid cancer incidence trends by histology in 25 countries: a population-based study [J]. Lancet Diabetes Endocrinol, 2021, 9(4): 225-234.
[13] Sciuto R, Romano L, Rea S, et al. Natural history and clinical outcome of differentiated thyroid carcinoma: a retrospective analysis of 1503 patients treated at a single institution [J]. Ann Oncol, 2009, 20(10): 1728-1735.
[14] Collet JF, Lacave R, Hugonin S, et al. BRAF V600E detection in cytological thyroid samples: a key component of the decision tree for surgical treatment of papillary thyroid carcinoma [J]. Head Neck, 2016, 38(7): 1017-1021.
[15] Hay ID, McConahey WM, Goellner JR. Managing patients with papillary thyroid carcinoma: insights gained from the Mayo Clinics experience of treating 2,512 consecutive patients during 1940 through 2000[J]. Trans Am Clin Climatol Assoc, 2002, 113: 241-260.
[16] 杨粒芝, 孙霄, 商蒙蒙, 等. 基于中国版甲状腺影像报告与数据系统的甲状腺结节恶性风险预测模型[J]. 山东大学学报(医学版), 2022, 60(6): 64-69. YANG Lizhi, SUN Xiao, SHANG Mengmeng, et al. C-TIRADS-based nomogram for malignant risk prediction of thyroid nodules [J]. Journal of Shandong University(Health Sciences), 2022, 60(6): 64-69.
[17] Haugen BR, Alexander EK, Bible KC, et al. 2015 American thyroid association management guidelines for adult patients with thyroid nodules and differentiated thyroid cancer: the American thyroid association guidelines task force on thyroid nodules and differentiated thyroid cancer [J]. Thyroid, 2016, 26(1): 1-133.
[18] Alexander EK, Doherty GM, Barletta JA. Management of thyroid nodules [J]. Lancet Diabetes Endocrinol, 2022, 10(7): 540-548.
[19] Alexander EK, Doherty GM, Barletta JA. Diagnosis of thyroid nodules [J]. Lancet Diabetes Endocrinol, 2022, 10(7): 533-539.
[20] Xing M, Tufano RP, Tufaro AP, et al. Detection of BRAF mutation on fine needle aspiration biopsy specimens: a new diagnostic tool for papillary thyroid cancer [J]. J Clin Endocrinol Metab, 2004, 89(6): 2867-2872.
[21] Xing M, Alzahrani AS, Carson KA, et al. Association between BRAF V600E mutation and mortality in patients with papillary thyroid cancer [J]. JAMA, 2013, 309(14): 1493-1501.
[22] Poulikakos PI, Sullivan RJ, Yaeger R. Molecular pathways and mechanisms of BRAF in cancer therapy [J]. Clin Cancer Res, 2022, 29: clincanres.2138.2021. doi: 10.1158/1078-0432.CCR-21-2138.
[23] Nikiforov YE, Nikiforova MN. Molecular genetics and diagnosis of thyroid cancer [J]. Nat Rev Endocrinol, 2011, 7(10): 569-580.
[24] Grogan RH, Mitmaker EJ, Clark OH. The evolution of biomarkers in thyroid cancer-from mass screening to a personalized biosignature [J]. Cancers(Basel), 2010, 2(2): 885-912.
[25] Li C, Lee KC, Schneider EB, et al. BRAF V600E mutation and its association with clinicopathological features of papillary thyroid cancer: a meta-analysis [J]. J Clin Endocrinol Metab, 2012, 97(12): 4559-4570.
[26] Zatelli MC, Trasforini G, Leoni S, et al. BRAF V600E mutation analysis increases diagnostic accuracy for papillary thyroid carcinoma in fine-needle aspiration biopsies [J]. Eur J Endocrinol, 2009, 161(3): 467-473.
[27] Kim SW, Lee JI, Kim JW, et al. BRAFV600E mutation analysis in fine-needle aspiration cytology specimens for evaluation of thyroid nodule: a large series in a BRAFV600E-prevalent population [J]. J Clin Endocrinol Metab, 2010, 95(8): 3693-3700.
[1] YANG Lizhi, SUN Xiao, SHANG Mengmeng, GUO Lu, SHI Dandan, LI Jie. C-TIRADS-based nomogram for malignant risk prediction of thyroid nodules [J]. Journal of Shandong University (Health Sciences), 2022, 60(6): 64-69.
[2] SHI Fei, PAN Xiang, YANG Jianming. Analysis of life quality in 106 papillary thyroid carcinoma patients undergoing total thyroidectomy [J]. Journal of Shandong University (Health Sciences), 2020, 58(5): 87-92.
[3] LI Jinye, SONG Gesheng, SONG Jiqing, WANG Dawei, JIN Xianwen, ZHANG Chengqi. Comparative study of gemstone spectral CT and conventional ultrasound spectroscopy for the diagnosis of benign and malignant thyroid nodules [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 81-86.
[4] LIU Yanxun, LIU Jia, ZHANG Tao, WANG Lu, XUE Fuzhong, WANG Ping. Type 2 diabetes mellitus associated with thyroid nodules: a longitudinal health check-up cohort study [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(8): 83-86.
[5] JIANG Lili, MA Zhe, MA Chuyun, HE Yuanliu, TAO Guowei, LIU Cun, GENG Qun, TANG Tingting, WANG Yin. Value of acoustic radiation force impulse imaging technology for differentiating benign from malignant thyroid nodules [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(10): 72-76.
[6] LI Wan-hu, CUI Yong-chun, WANG Xiao-yue, LIANG Wen-juan. Application of ultrasonography to the diagnosis of Hashimoto′s thyroiditis with papillary thyroid carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(9): 140-143.
[7] LI Jian-zhou, LIU Xin, ZHANG Ling-yun, JIN Yong-jun. Relationship between preoperative serum TSH and  differentiated thyroid carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(1): 10-13.
[8] QIAO Ling-yan1, SONG Xin-hong2, LIN Hai-yan2, GAO Ling3, ZHAO Jia-jun1. Prevalence of thyroid nodules in healthy population [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(8): 5-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1 -6 .
[2] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32 -37 .
[3] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7 -14 .
[4] FU Jieqi, ZHANG Man, ZHANG Xiaolu, LI Hui, CHEN Hong. Molecular mechanism of Toll-like receptor 4 in the aggravation of blood lipid accumulation by inhibiting the peroxisome proliferator-activate receptor γ[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 24 -31 .
[5] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15 -23 .
[6] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38 -46 .
[7] XU Yuxiang, LIU Yudong, ZHANG Peng, DUAN Ruisheng. A retrospective analysis of risk factors of cerebral microbleeds in 101 patients with cerebral small vessel disease[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 67 -71 .
[8] DING Xiangyun, YU Qingmei, ZHANG Wenfang, ZHUANG Yuan, HAO Jing. Correlation of the expression of insulin-like growth factor II in granulosa cells and ovulation induction outcomes of 84 patients with polycystic ovary syndrome[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 60 -66 .
[9] XIAO Juan, XIAO Qiang, CONG Wei, LI Ting, DING Shouluan, ZHANG Yuan, SHAO Chunchun, WU Mei, LIU Jianing, JIA Hongying. Comparison of diagnostic efficacy of two kinds of thyroid imagine reporting and data systems[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 53 -59 .
[10] SHI Shuang, LI Juan, MI Qi, WANG Yunshan, DU Lutao, WANG Chuanxin. Construction and application of a miRNAs prognostic risk assessment model of gastric cancer[J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 47 -52 .
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd