Journal of Shandong University (Health Sciences) ›› 2020, Vol. 1 ›› Issue (7): 38-46.doi: 10.6040/j.issn.1671-7554.0.2020.0557

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Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion

LI Ning1,2, LI Juan1,2, XIE Yan1,2, LI Peilong1,2, WANG Yunshan1,2, DU Lutao1,2, WANG Chuanxin1,2   

  1. 1. Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250033, Shandong, China;
    2. Tumor Marker Detection Engineering Laboratory of Shandong Province, Jinan 250033, Shandong, China
  • Online:2020-07-20 Published:2020-07-10

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Abstract: Objective To investigate the expression of long non-codingRNAs(LncRNAs)AL109955.1 in colorectal cancer(CRC)tissues and its effect on cell proliferation, migration and invasion. Methods The cancer genome atlas(TCGA)and genotype-tissue expression(GTEx)were analyzed to screen LncRNAs which were decreased in CRC tissues and related to the poor prognosis of patients, and then the expression in 80 pairs of CRC tissues and adjacent normal tissues was verified by quantitative real-time PCR(RT-qPCR). After that, the cell proliferation, migration and invasion were detected with cell counting kit-8(CCK8)test, wound healing test and Transwell assay, respectively. Finally, the miRNA binding sites and target mRNAs were screened with TargetScan and Tarbase, and a LncRNA-miRNAs-mRNA network was constructed with Cytoscape software(3.7.2). Results A novel LncRNA, AL109955.1, was screened and identified, and its expression was significantly lower in CRC tissues than that in adjacent normal tissues ［(1.18±2.46)vs(1.8±1.65), t=2.14, P=0.008］. AL109955.1 expressed differently in CRC tissues with different degrees of differentiation ［poorly differentiated: 0.18(0.10~0.55); moderately differentiated: 0.49(0.22~1.81); well differentiated: 0.87(0.15~3.33)］. In addition, AL109955.1 expression decreased with the increase of tumor size ［≥5 cm: 0.26(0.11~0.62); <5 cm: 0.49(0.181~1.99), U=570.5, P=0.020)］. CCK8 test showed AL109955.1 could significantly inhibit the proliferation of CRC cells. Wound healing test and Transwell assay showed overexpression of AL109955.1 significantly decreased the migration and invasion of CRC cells. Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analysis indicated that AL109955.1 played its biological role by affecting classical tumor signaling pathways such as Wnt, p53, Notch and Jak-STAT. Conclusion LncRNA AL109955.1 expression is lower in CRC tissues than that in adjacent normal tissues. LncRNA AL109955.1 can inhibit the proliferation, migration and invasion abilities of CRC cells.

Key words: Colorectal cancer, Non-coding RNA, Proliferation, Migration, Invasion

CLC Number: 

  • R737.9
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