Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (3): 63-68.doi: 10.6040/j.issn.1671-7554.0.2018.924

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Expression and clinical significance of tripartite motif protein 26 in glioma tissues

ZHAO Xueying1, FAN Mingde2, DONG Wenyan3, QI Yingzhi1, LU Xin1   

  1. 1. Depatment of Blood Transfusion;
    2. Department of Neurosurgery;
    3. Department of Cadre Health, The Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To detect the expression of tripartite motif protein 26(TRIM26)in glioma tissues, to analyze the relationship of its expression and multiple pathological parameters, and to explore the effect on its overexpression in U87 and U251 for the proliferation of glioma cells. Methods A total of 32 cases of glioma tissues and 8 cases of normal brain tissues were collected. Real-time quantitative PCR and immunohistochemistry were used to detect the expression of TRIM26 in levels of mRNA and protein. The relationship between TRIM26 mRNA expression and clinical pathological parameters was analyzed. Effect on TRIM26 overexpression in U87 and U251 for proliferation of glioma cells was detected by MTT assay. Results Immunochemistry results showed that the percentage of TRIM26 positive cells was higher in glioma tissues than control tissues. Compared with the control group, the expression of TRIM26 in glioma tissues was significantly increased ［3.51±0.92 vs 1.99±0.45, P<0.01］. The expression of TRIM26 in patients of Ⅲ-Ⅳ grade was markedly higher than in patients of Ⅰ-Ⅱ grade ［3.75±0.84 vs 2.65±0.62, P<0.01］. TRIM26 expression was observably associated with stage(P=0.01), but not gender(P=0.72), age(P=0.72), and IDH1 mutation(P>0.999). Moreover, Kaplan-Meier analysis demonstrated that increasing of TRIM26 expression contributed to shorter overall survival ［(16.29±1.82)vs(28.73±1.41)months, P<0.01］ and recurrence-free survival time ［(8.33±1.78)vs(15.95±1.83)months, P<0.01］. Overexpression of TRIM26 in U87 and U251 cell lines promoted the proliferation of glioma cells. Conclusion TRIM26 is highly expressed in glioma, and the overexpression of TRIM26 is associated with pathological grade and prognosis.

Key words: Tripartite motif protein 26, Glioma, Pathological parameters, Prognosis, Proliferation

CLC Number: 

  • R739.41
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