Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (10): 101-106.doi: 10.6040/j.issn.1671-7554.0.2019.455

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Diagnosis value of serum exosome miR-205-5p/miR-152-5p on early non-small cell lung cancer

ZHENG Qingyue1, ZHAO Qiuhong1, QU Xiangyun2, DONG Zhaonan2, MA Xueqing2, JIA Yunli2   

  1. 1. Respiratory Medicine Department, Beijing Aerospace General Hospital, Beijing 100076, China;
    2. Jiangsu Engineering Research Center for Tumor Molecular Diagnosis, Suzhou 215123, Jiangsu, China
  • Published:2022-09-27

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Abstract: Objective To study the comprehensive expression and diagnosis value of serum exosomal miR-205-5p and miR-152-5p for early non-small cell lung cancer(NSCLC). Methods The objects were divided into the NSCLC group (n=83), which were further divided into the adenocarcinoma group (n=55) and squamous cell carcinoma group (n=28), and the control group (n=50). The TNM stage of NSCLC patients was 0-II. Serum samples were collected, the exosomal miRNA were extracted, then the comprehensive expressions of miR-205-5p and miR-152-5p were detected. The comprehensive expression between the NSCLC group and control group, and among adenocarcinoma group, squamous cell carcinoma group and control group were compared. Multiple linear regression was used to analyze the correlation between expression level and gender, age, smoking history, tumor size and lymph node metastasis. The diagnostic 山 东 大 学 学 报 (医 学 版)57卷10期 -郑清月,等.血清外泌体miR-205-5p/miR-152-5p对早期非小细胞肺癌的诊断价值 \=-value of the comprehensive expression of miR-205-5p and miR-152-5p was evaluated with receiver operating characteristic(ROC)curve. Results The comprehensive expressions of control group, NSCLC group, adenocarcinoma group and squamous carcinoma group were 17.76±4.27, 20.52±5.51, 21.79±5.12, and 18.02±5.48, respectively. The expression was significantly higher in the NSCLC group than that in the control group (t=3.027,P=0.003). There were statistically significant differences among adenocarcinoma group, squamous cell carcinoma group and control group (F=10.412,P<0.001). The comprehensive expression of miR-205-5p/miR-152-5p was significantly higher in the adenocarcinoma group than that in the squamous cell carcinoma group (P=0.001) and the control group (P<0.001). The expression in the squamous cell carcinoma group and control group had no difference (P=0.823). Multiple linear regression analysis showed correlation between lymph node metastasis and expression level (P=0.037). For evaluation of diagnostic value of NSCLC, the area under the ROC curve(AUC)was 0.671(95%CI: 0.579-0.764),the cutoff value was 18.493, and the sensitivity and specificity were respective 73.3% and 64.0%. For evaluation of diagnostic value of lung adenocarcinoma, the AUC was 0.738(95%CI:0.642-0.883), the cutoff value was 18.495, the sensitivity and specificity were respective 78.2% and 64.0%. There was no diagnostic value for lung squamous cell carcinoma, with the AUC being 0.541(95%CI:0.399-0.684). Conclusion The comprehensive relative expressions of serum exosomal miR-205-5p and miR-152-5p are increased in early NSCLC patients, mainly in early lung adenocarcinoma patients. It has auxiliary value for early diagnosis of NSCLC.

Key words: miRNA-205-5p, miRNA-152-5p, Lung adenocarcinoma, Lung squamous cell carcinoma, Non-small cell lung cancer

CLC Number: 

  • R734.2
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