Abstract: Objective To investigate whether the serum S100B protein and neuron-specific enolase(NSE)levels were associated with neonatal hypoglycemic brain damage(HBD). Methods A total of 48 hypoglycemic neonates admitted during Oct. 2014 and Sept. 2015 were selected as the experimental group, who were divided into 2 subgroups according to clinical manifestations and EEG examination: symptomatic hypoglycemic group(n=22)and asymptomatic hypoglycemic group(n=26). Another 40 healthy subjects served as the control group. Blood samples were collected from all neonates 24 and 72 hours after birth. The levels of serum S100B protein and NSE protein were measured with Human S100B Elisa kit and Cobas 411 electro-chemiluminescence immunoassay analyzer, respectively. EEG examination was performed after admission. Results The level of blood glucose in the symptomatic group was lower than that of asymptomatic group and control group(P<0.01), and that of the asymptomatic group was lower than the control 山 东 大 学 学 报 (医 学 版)54卷4期 -臧丽娇,等.血清S100B蛋白、神经元特异性烯醇化酶与新生儿低血糖脑损伤的相关性 \=-group(P<0.05). The duration of hypoglycemia was longer in the symptomatic group than in the asymptomatic group(P<0.01). The symptomatic group had significantly increased levels of serum 24 h-NSE and 24 h-S100B than the other 2 groups(P<0.01), and the asymptomatic group had higher levels than the control group(P<0.05). There were no statistical differences in the levels of serum 72 h-NSE and 72 h-S100B among the 3 groups(P>0.05). The level of serum 24 h-NSE was negatively correlated with 24 h-S100B, positively correlated with the duration of hypoglycemia(P<0.05), and the serum NSE was positively correlated with S100B protein(P<0.05). Conclusion The levels of serum S100B protein and NSE elevate in neonatal HBD, which are closely associated with the severity and duration of hypoglycemia, suggesting that they may serve as early sensitive markers for the diagnosis of neonatal HBD.

Key words: Neonate, Hypoglycemic brain damage, S100B protein, Neuron-specific enolase