JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (4): 37-42.doi: 10.6040/j.issn.1671-7554.0.2013.709

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Neuroprotection of sodium pyruvate on repetitive and profound neonatal hypoglycemic brain injury

ZHOU Dong1, CHANG Hong2, SUN Ruopeng1   

  1. 1. Department of Pediatrics, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. The Affiliated Hospital of Qingdao University, Qingdao 266001, Shandong, China
  • Received:2013-11-25 Revised:2015-03-10 Online:2015-04-10 Published:2015-04-10

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Abstract: Objective To investigate the neuroprotective effect of sodium pyruvate on newborn Wistar rats suffered from repetitive and profound insulin-induced hypoglycemic brain injury. Methods A total of 36 newborn Wistar rats were randomly divided into three groups: insulin-treated P group (INS-P group, n=12), insulin and sodium pyruvate-treated group (INS-PP group, n=12), and control group (n=12). On postnatal day 2, 4 and 6, insulin were injected hypodermicly into rats to induce hypoglycemia. After hypoglycemia lasting for 2.5 hours, dextrose was injected hypodermicly into rats in INS-P group, while dextrose was injected hypodermicly and sodium pyruvate was injected peritoneally into rats in INS-PP group. On 1 day after the third hypoglycemic insult, the neuronal degeneration in the rats' brains was examined by FJB staining. When the rats were 6 weeks old, their spatial learning and memory ability were detected with Morris' water maze experiment. Results Compared with INS-P group, the number of degenerated neurons in INS-PP group reduced and spatial learning and memory ability decreased. For the rats in INS-PP group, the spending time to find the platform was less, swimming distance was shorter, lingering time in the quadrant of platform was longer, and times to cross the platform were increased compared with those in INS-P group. Conclusion Sodium pyruvate can provide a neuroprotective effect on rats suffered from repetitive and profound neonatal hypoglycemia. Sodium pyruvate administration may be an effective intervention for patients with severe neonatal hypoglycemia to prevent brain injury.

Key words: Rat, Wistar, Neonate, Hypoglycemia, Neurodegeneration, Cognitive function

CLC Number: 

  • R722
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