JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 73-76.doi: 10.6040/j.issn.1671-7554.0.2014.600

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Clinical significance of human telomerase reverse transcriptase gene methylation status in urine sediments of renal cell carcinoma

REN Hongbo, LIU Hainan, LIU Cheng, YAN Keqiang, XIA Chuanyou, FAN Yidong   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-09-11 Revised:2014-11-25 Published:2015-01-10

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Abstract: Objective To elucidate the methylation status of human telomerase reverse transcriptase (hTERT) promoter in urine sediments obtained from preoperative patients with renal cell carcinoma (RCC). Methods Genomic DNA was extracted from urine sediments of 76 RCC patients (RCC group) and 38 non-renal cell carcinoma patients (control group). Specimen DNA was modified with sodium bisulfite, methylation specific PCR (MSP) was carried out, and agar gel electrophoresis was performed to analyze the methylation status of hTERT gene. Results The rate of hTERT hypermethylation in RCC group was obviously higher than that in control group (P< 0.05). But no statistical correlation was observed between hTERT promoter methylation and gender, age, tumor size or clinical stage (P>0.05). Conclusion hTERT promoter in urine sediment of renal cell carcinoma patients is highly methylated, and as a noninvasive investigation, it might be useful in the clinical diagnosis of renal cell carcinoma.

Key words: Renal neoplasms, Methylation, Human telomerase reverse transcriptase, Urine sediments

CLC Number: 

  • R737.11
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