烟曲霉菌提取物对人支气管上皮细胞修复功能的影响

doi:10.6040/j.issn.1671-7554.0.2014.506

Abstract

Abstract: Objective To investigate the effects of aspergillus fumigatus extract (AFE) on the human bronchial epithelial cells repair ability and its possible mechanism. Methods The cells were divided into medium control group, different concentrations (10, 20 and 40 mg/L) of AFE groups and heat-treated AFE group. Human bronchial epithelial cells 16HBE were cultured in vitro, which were exposed to different concentrations of AFE for different time. Wound closure assay, cell migration, quantitative cell attachment assay and spreading of bronchial epithelial cells were observed. AFE and heat-treated AFE were used to investigate the effects of AFE on the human bronchial epithelial cells repair ability. Results Compared with medium control group, the repair capacity, migration ability and adhesion ability of 16HBE cells were inhibited significantly in 20 and 40 mg/L groups (P<0.05) with a time- and concentration-dependent manner; however, there was no obvious change in 10 mg/L AFE group (P>0.05). The spreading ability of 16HBE cells in 10, 20 and 40 mg/L AFE groups were inhibited with a concentration-dependent manner (P<0.05). Heat-treated AFE, whose protease activities were lost, showed no effects on the indicators mentioned above (P<0.05). Conclusion AFE, depending on its protease activity, can inhibit the repair ability of human bronchial epithelial cells, which is positively related to the exposure time and concentration of AFE.

Key words: Repair ability, Aspergillus fumigatus, Bronchial epithelial cell, Protease

CLC Number:

R363

LONG Fei, SU Lili, LIANG Bin, JIN Xianqiao. Effects of Aspergillus fumigatus extract on human bronchial epithelial cells repair ability[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(11): 21-26.

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Effects of Aspergillus fumigatus extract on human bronchial epithelial cells repair ability

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