JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 27-32.doi: 10.6040/j.issn.1671-7554.0.2014.561

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Fertility restore of allotransplantation of vitrified-warmed junvenile ovaries treated with a modified vitrification solution

WANG Hongyan1,2, WANG Yanrong1, XU Xian1,3, ZHAO Junli1,3, HA Lingxia1,3, JIA Shaotong1,3, LIU Chunlian1,3   

  1. 1. Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004 Ningxia, China;
    2. Human Sperm Bank of The Total Hospital, Ningxia Medical University, Yinchuan 750004 Ningxia, China;
    3. Center of Reproductive Medicine of Total Hospital, Ningxia Medical University, Yinchuan 750004 Ningxia, China
  • Received:2014-08-26 Published:2014-11-10

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Abstract: Objective To explore the development and fertility potential of oocytes retrieved from surviving prepubertal mice ovarian grafts treated with orthotopic transplantation after a modified solution cryopreservation by vitrification. Methods Prepubertal mice ovaries were cryopreserved by vitrification using ED20 and modified EG5.5/30 solutions. Part of the fresh and vitrified-warmed ovaries were treated with enzyme digestion to test the survival rate of eggs with fluorescence dynamic method. The fluorescence intensity quantitative analysis of microfilament and microtubules of GV oocytes were evaluated with laser confocal microscopy. Part of fresh and vitrified-warmed ovaries were allotransplanted under the ovarian capsule of ovariectomized adult female mice recipients. All juvenile mice were randomly divided into six groups: fresh group and fresh orthotopic transplantation group; ED20 solution vitrified-warmed group and ED20 solution vitrified-warmed orthotopic transplantation group; modified EG5.5/30 solution vitrified-warmed group and modified EG5.5/30 solution vitrified-warmed orthotopic transplantation group. To evaluate the fertility and developmental potential of the fresh and vitrified-warmed surviving ovarian grafts, 15 days after orthotopic transplantation, female micerecipients that restored estrous cycle were mated with male mice and serum levels of estradiol (E2) in female mice recipients was measured. Results Vitrified-warmed prepubertal mice ovaries showed a high survival rate of oocytes although a remarkable reduction from 92.6% (fresh group) to 87.0% (ED20 solution vitrified-warmed group) and 84.6% (EG5.5/30 solution vitrified-warmed group) was observed respectively (P<0.05). Results of quantitative analysis of microfilament and microtubules of GV oocytes decreased significantly in the two cryopreserved groups when compared with those of fresh group (P<0.05). Female mice recipients that received vitrified-warmed juvenile ovaries could successfully give birth after mating. The first litter time, litter size and total litter size of the two vitrified-warmed orthotopic transplantation groups showed marked differences compared with fresh orthotopic transplantation group (P<0.05). Conclusion Juvenile mice ovaries treated with cryopreservation by vitrification in the modified EG5.5/30 solution and allotransplantation do not affect the development of eggs and reproductive potential, similar in ED20 solution. Female mice recipients can grive normal offspring, but reproductive reserve and fertility significantly decreased.

Key words: Orthotopic transplantation, Juvenile mouse ovary, Vitrification, In vitro fertilization, Reproductive potential

CLC Number: 

  • R737.3
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