JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (11): 27-32.doi: 10.6040/j.issn.1671-7554.0.2014.561

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Fertility restore of allotransplantation of vitrified-warmed junvenile ovaries treated with a modified vitrification solution

WANG Hongyan1,2, WANG Yanrong1, XU Xian1,3, ZHAO Junli1,3, HA Lingxia1,3, JIA Shaotong1,3, LIU Chunlian1,3   

  1. 1. Key Laboratory of Fertility Preservation and Maintenance, Ministry of Education, Ningxia Medical University, Yinchuan 750004 Ningxia, China;
    2. Human Sperm Bank of The Total Hospital, Ningxia Medical University, Yinchuan 750004 Ningxia, China;
    3. Center of Reproductive Medicine of Total Hospital, Ningxia Medical University, Yinchuan 750004 Ningxia, China
  • Received:2014-08-26 Published:2014-11-10

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Abstract: Objective To explore the development and fertility potential of oocytes retrieved from surviving prepubertal mice ovarian grafts treated with orthotopic transplantation after a modified solution cryopreservation by vitrification. Methods Prepubertal mice ovaries were cryopreserved by vitrification using ED20 and modified EG5.5/30 solutions. Part of the fresh and vitrified-warmed ovaries were treated with enzyme digestion to test the survival rate of eggs with fluorescence dynamic method. The fluorescence intensity quantitative analysis of microfilament and microtubules of GV oocytes were evaluated with laser confocal microscopy. Part of fresh and vitrified-warmed ovaries were allotransplanted under the ovarian capsule of ovariectomized adult female mice recipients. All juvenile mice were randomly divided into six groups: fresh group and fresh orthotopic transplantation group; ED20 solution vitrified-warmed group and ED20 solution vitrified-warmed orthotopic transplantation group; modified EG5.5/30 solution vitrified-warmed group and modified EG5.5/30 solution vitrified-warmed orthotopic transplantation group. To evaluate the fertility and developmental potential of the fresh and vitrified-warmed surviving ovarian grafts, 15 days after orthotopic transplantation, female micerecipients that restored estrous cycle were mated with male mice and serum levels of estradiol (E2) in female mice recipients was measured. Results Vitrified-warmed prepubertal mice ovaries showed a high survival rate of oocytes although a remarkable reduction from 92.6% (fresh group) to 87.0% (ED20 solution vitrified-warmed group) and 84.6% (EG5.5/30 solution vitrified-warmed group) was observed respectively (P<0.05). Results of quantitative analysis of microfilament and microtubules of GV oocytes decreased significantly in the two cryopreserved groups when compared with those of fresh group (P<0.05). Female mice recipients that received vitrified-warmed juvenile ovaries could successfully give birth after mating. The first litter time, litter size and total litter size of the two vitrified-warmed orthotopic transplantation groups showed marked differences compared with fresh orthotopic transplantation group (P<0.05). Conclusion Juvenile mice ovaries treated with cryopreservation by vitrification in the modified EG5.5/30 solution and allotransplantation do not affect the development of eggs and reproductive potential, similar in ED20 solution. Female mice recipients can grive normal offspring, but reproductive reserve and fertility significantly decreased.

Key words: Orthotopic transplantation, Juvenile mouse ovary, Vitrification, In vitro fertilization, Reproductive potential

CLC Number: 

  • R737.3
[1] Gamzatova Z, Komlichenko E, Kostareva A, et al. Autotransplantation of cryopreserved ovarian tissue-effective method of fertility preservation in cancer patients[J]. Gynecol Endocrinol, 2014, 30( Suppl 1):43-50.
[2] Donnez J, Dolmans M M. Fertility preservation in women[J]. Nat Rev Endocrinol, 2013, 9(12):735-749.
[3] Gosden R. Cryopreservation: a cold look at technology for fertility preservation[J]. Fertil Steril, 2011, 96(2):264-272.
[4] Meirow D, Ra'anani H, Biderman H. Ovarian tissue cryopreservation and transplantation: a realistic, effective technology for fertility preservation[J]. Methods Mol Biol, 2014,1154:455-463. doi: 10.1007/978-1-4939-0659-8_21.
[5] Herraiz S, Novella-Maestre E, Rodríguez B, et al. Improving ovarian tissue cryopreservation for oncologic patients: slow freezing versus vitrification, effect of different procedures and devices[J]. Fertil Steril, 2014, 101(3):775-784.
[6] Xiao Z, Wang Y, Li L L, Li S W. In vitro culture thawed human ovarian tissue: NIV versus slow freezing method[J]. Cryo Letters, 2013, 34(5):520-526.
[7] Nikiforaki D, Vanden Meerschaut F, Qian C, et al. Oocyte cryopreservation and in vitro culture affect calcium signalling during human fertilization[J]. Hum Reprod, 2014, 29(1):29-40.
[8] Nayot D, Chung J T, Son W Y, et al. Live birth following serial vitrification of embryos and PGD for fragile X syndrome in a patient with the premutation and decreased ovarian reserve[J]. J Assist Reprod Genet, 2013, 30(11):1439-1444.
[9] 王红燕,秦璟,朱万平,等. 3种玻璃化液对新生鼠卵巢的渗透反应及冻存效果的比较[J].生殖与避孕,2009,29(8):508-512. WANG Hongyan, QIN Jing, ZHU Wanping, et al. Comparison of Osmotic Respond and Cryoprotective effect of three vitrification solutions on newbornrat ovaries[J]. Reproduction and Contraception, 2009, 29(8):508-512.
[10] 王红燕,秦璟,王燕蓉,陈子江.三种玻璃化液对新生大鼠卵巢玻璃化冷冻效果的比较[J].山东大学学报:医学版,2011,49(12):34-38. WANG Hongyan, QIN Jing, WANG Yanrong, et al. Comparison of the cryoprotective effect of three vitrification solutions on new born rat ovaries[J]. Journal of Shandong University: Health Sciences, 2011, 49(12):34-38.
[11] 王艳盛,谢莎莎,王燕蓉,等.促卵泡激素干预改善绵羊卵巢组织玻璃化冻存后异种移植的效果研究[J].中国组织工程研究与临床康复, 2010,14(18):3301-3304. WANG Yansheng, XIE Shasha, WANG Yanrong, et al. Effect of follicle stimulating hormone on cryopreserved sheep ovarian tissue xenotransplantation[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2010, 14(18):3301-3304.
[12] 吴昊, 蔡玉芳,王燕蓉,等.FSH干预对小鼠玻璃化冻融移植卵巢血流重建的影响[J].宁夏医科大学学报,2014,36(5):478-482.
WU Hao, CAI Yufang, WANG Yanrong, et al. The effects of FSH intervention in vitrification freezing on the revascularization after mouse ovarian transplantation[J]. Journal of NingxiaMedical University, 2014, 36(5):478-482.
[13] Wang H Y, Li Y H, Sun L, et al. Allotransplantation of cryopreserved prepubertal mouse ovaries restored puberty and fertility without affecting methylation profile of Snrpn-DMR[J]. Ferti Steril, 2013,99(1):241-247.
[14] Sugimoto M, Maeda S, Manabe N, Miyamoto H. Development of infantile rat ovaries autotransplanted after cryopreservation by vitri cation[J]. Theriogenology, 2000, 53(5):1093-1193.
[15] Youm H W, Lee J R, Lee J, et al. Optimal vitrification protocol for mouse ovarian tissue cryopreservation: effect of cryoprotective agents and in vitro culture on vitrified-warmed ovarian tissue survival[J]. Hum Reprod, 2014, 29(4):720-730.
[16] Newton H, Fisher J, Arnold J R, et al. Permeation of human ovarian tissue with cryoprotective agents in preparation for cryopreservation[J]. Hum Reprod, 1998, 13(2):376-380.
[17] Fathi R, Valojerdi M R, Salehnia M. Effects of different cryoprotectant combinations on primordial follicle survivability and apoptosis incidence after vitrification of whole rat ovary[J]. Cryo Letters, 2013, 34(3):228-238.
[18] Courbiere B, Odageseu V, Baudot A, et al. Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols[J]. Fertil Steril, 2006, 86(4 Suppl):1243- 1251.
[19] Chen C K, Wang C W, Tsai W J, et al. Evaluation of meiotic spindles in thawed oocytes after vitrification using polarized light microscopy[J]. Ferti Steril, 2004, 82(3):666-672.
[20] Sun Q Y, Lai L X, Park K W, et al. Dynamic events are differently mediated by microfilaments, microtubules, and mitogen-activated protein kinase during porcine oocyte maturation and fertilizationin vitro[J]. Biol Reprod, 2001, 64 (3):879-889.
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