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Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 after diffuse brain injury and their significance in rats

SONG Lin-jie1, ZHANG Xiu-ping2, WANG Chong1, DONG Ya-nan1, JIANG Yong1, ZHAO Hong-yang3, ZHU Xian-li3   

  1. 1. Deparment of Neurosurgery, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
    2. Central Laboratory, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
    3. Deparment of Neurosurgery, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China
  • Received:2008-07-09 Revised:1900-01-01 Online:2008-11-16 Published:2008-11-16
  • Contact: SONG Lin-jie

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Abstract: To explore whether expressions of matrix metalloproteinase-9 and tissuce inhibitor of metalloproteinase-1 in early diffuse brain injured tissues have a correlation with second brain injury in rats. Methods Real-Time quantitative PCR was used to determine expressions of MMP-9mRNA and TIMP-1mRNA, the dry-wet method to determine the containing water measure in the brain, and histological techniques and electron microscopy to determine the ultrastructural blood brain barrier at different time-points after diffuse brain injury in rats. Results Compared with the sham-operated group, expression of MMP-9mRNA started to increase at the first hour, peaked at the 12th hour(P<0.01) and persisted up to one week. Expression of TIMP-1mRNA also peaked at the 24th hour and persisted up to one week (P<0.01). Ultrastructures of the neurons were injured, edema and necrosis were diffuse and distinct at the 72 th hour under light microscopy and electron microscopy. ConclusionsExpressions of MMP-9mRNA and TIMP-1mRNA are increased after diffuse brain injury in rats, indicating that MMP-9 and TIMP-1 are closely related to brain edema and neuronal damage in early second brain injury in rats.

Key words: Brain injury, Gelatinasc B, Tissue inhibitor of metalloproteinase1, Blood brain barrier, Rats, sprague-dawley

CLC Number: 

  • R651.1
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