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Changes of expression of mitochondrial cytochrome oxidase subunits Ⅲ and Ⅳ in the rat hippocampus of temporal lobe epilepsy

GAO Jing1，CHEN Wen2，ZHANG Tong-xia3，WANG Xiao-hua1，DAI Ting-jun1，YAO Hong1，ZHAO Xiu-he1，CHI Zhao-fu1，SHAN Pei-yan1   

  1. 1. Department of Neurology, Qilu Hospital of Shandong University；2. Department of Neurology, Jinan Central Hospital；3. School of Medicine, Shandong University
  • Received:2006-11-26 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24
  • Contact: CHI Zhao-fu

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Abstract: Objective: To investigate the effect of epilepsy on expressions of cytochrome oxidase(COX) subnuits Ⅲ(COX Ⅲ) and Ⅳ (COX Ⅳ) encoded by mtDNA and nDNA in the rat hippocampus. Methods: Forty male Wistar rats were randomly divided into the saline control group, the acute period group (3h), the silent period group（7d）, the chronic period group(45d) after status epilepticus(SE) and the group which received pilocarpine (PILO) but did not develop SE(n=8 per group). Expressions of COX Ⅲ and COX Ⅳ mRNA and protein in the rat hippocampus were respectively determined by real time quantitative PCR(RQ-PCR) and Western blot. Results: COX Ⅲ mRNA and protein were significantly increased in the group at 3h after SE(P<0.001, P<0.01) compared with the control group. Conclusion: Dysfunction of COX in the hippocampus is related to prolonged seizure during experimental temporal lobe epilepsy and the mitochondria are vulnerable to epilepsy.

Key words: Cytochrome oxidase, Epilepsy, Hippocampus, Mitochondria, Rats, Wistar

CLC Number: 

  • R742.1
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