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In vivo vaccine effect and tumorigenicity of human breast carcinoma cells transfered by interleukin-18 gene

HAN Ming-yong1，LIU Qi1，TANG Bu-jian2，DENG Yan2，CAO Ming-feng1   

  1. 1. Department of Tumor Treatment Center, Shandong Provincial Hospital, Jinan 250021,Shandong, China; 2 Guangxi Medical University, Nanning 530021, Guangxi, China
  • Received:2006-03-29 Revised:1900-01-01 Online:2007-07-24 Published:2007-07-24
  • Contact: LIU Qi

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Abstract: Objective: To study the effect of the interleukin-18 gene transfection on tumorigenesis of the human breast carcinoma cell line Bcap37. Methods: Bcap37 cells were transfected with a mammalin expression vector containing human IL-18 complementary DNA. The biological expression of IL-18 was determined by the RT-PCR and ELISA methods. Nude mice were injected with Bcap37 cells with or without the IL-18 gene. Results: The IL-18 cDNA was successfully integrated into the Bcap37 cells. There were (113.8±4.7)pg IL-18 secreted by one million transduced cells in 24 hours. In vivo tumor growth curves, based on measured tumor size, Bcap37, Bcap37-pcDNA3.1 and Bcap37-IL-18 resulted in tumor formation from 8 to 11 days after injection. The growth rate of Bcap37-IL-18 in vivo was significantly slower than that of Bcap37 and Bcap37-pcDNA3.1 cells. Tumor formation by parental Bcap37 cells was significantly delayed in mice that had been immunized with Bcap37-IL18 irradiated cells. Conclusion: Human breast carcinoma cells were successfully modified by IL-18 cytokine genes and reduced the tumorigenicity. Breast cells transduced with IL-18 genes might be used as human colon cancer vaccines.

Key words: Interleukin-18, Breast neoplasms, Transfection, Tumor cells, cultured, Vaccine

CLC Number: 

  • R739.9
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